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Molecular Mechanism of Cytoplasmic Male Sterility in Sugarbeet (Beta vulgaris L.)

Research Project

Project/Area Number 06454039
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

MIKAMI Tetsuo  Hokkaido Univ., Fac.of Agr., Professor, 農学部, 教授 (50133715)

Co-Investigator(Kenkyū-buntansha) INUKAI Tuyoshi  Hokkaido Univ., Fac.of Agr., Instructor, 農学部, 助手 (90223239)
KUBO Tomohiko  Hokkaido Univ., Fac.of Agr., Instructor, 農学部, 助手 (40261333)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywordssugarbeet / cytoplasmic male sterility / mitochondria / coxI / Rf / transcription / flower bud RNA / Northern blot analysis / 構造変異 / rps13 / rps3 / orf171 / orfB
Research Abstract

To study the molecular basis of cytoplasmic male sterility (CMS) in sugarbeet, we have conducted a detailed comparison of the mitochondrial genomes of two sugarbeet lines, one containing a fertile (N) cytoplasm and the other a male sterile one. The two genomes have approximately 330kb of common sequences but there is considerable variation in sequence organization which can be accounted for by genome rearrangements.
Transcripts for known mitochondrial genes and regions of the two sugarbeet genomes known to be rearranged relative to one another were compared among male-fertile, CMS and nuclear-restored sugarbeet genotypes. In most instances, identical transcript patterns were observed for all three, mtRNAs (prepared from leaves or flower buds), indicating that rearrangement events did not occur within transcriptional units. Altered transcript patterns were, however, identified for six genes, coxI,coxII,atpA,rps3, orf324, and orf1.
Interestingly, the coxI transcripts were found to be significantly affected by nuclear background, whereas unclear background has no effect on the transcripts of other five genes.
The results suggest that nuclear-restorer gene (s) have an effect on coxI transcript patterns and implicate coxI in sugarbeet male sterility.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kubo, T.: "The chloroplast trnP-trnW-petG gene cluster in the mitochondrial genomes of Beta vulgaris,B,trigyna and B.webbiana:evolutionary aspects" Current Genetics. 27. 285-289 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kubo, T.: "Physical and gene organization of mitochondrial DNA from the fertile cytoplasm of sugarbeet(Beta vulgaris L.)" Current Genetics. 28. 235-241 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Senda, M.: "Mitochondrial gene variation and phylogenetic relationships in the genus Beta" Theoretical and Applied Genetics. 90. 914-919 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, S.: "Mitochondrial DNA analysis reveals cytoplasmic variation within a single cultivar of perennial ryegrass(Lolium perenne L.)" Euphytica. 83. 205-208 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato, S.: "The apple mitochondrial atp9 gene:RNA editing and co-transcription with exons a and b of the nad5 gene" Physiologia Plantarum. 93. 572-575 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato, S.: "Nucleotide sequence and transcription of the gene coding for ATPase subunit 9 in apple plant(Malus x domestica Borkh.)" Acta Horticulturae. 392. 235-241 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kubo, T., Yanai, Y., Kinoshita, T., Mikami, T.: "Chloroplast trnP-trnW-petG gene cluster in the mitochondrial genomes of Beta vulgaris, B.trigyna and B.webbiana : evolutionary aspects." Curr.Genet.27. 285-289 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kubo, T., Satoh, Y., Muro, T., Kinoshita, T., Mikami, T.: "Physical and gene organization of mitochondrial DNA from the fertile cytoplasm of sugarbeet (Beta vulgalis L.) ." Current Genetics. 28. 235-241 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Senda, M., Onodera, Y., Kinoshita, T., Mikami, T.: "Mitochondrial gene variation and phylogenetic relationships in the genus Beta." Theor.Appl.Genet.90. 914-919 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, M., Yamashita, M., Kato, S., Mikami, T., Shimamoto, Y.: "Mitochondrial DNA analysis reveals cytoplasmic vatiability within a single cultivar of perennial ryegrass (Lolium perenne L.) ." Euphytica. 83. 205-208 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato, S., Shimamoto, Y., mikami, T.: "The apple mitochondrial gene : RNA editing and co-transcription with exons a and b of the nad5 gene." Physiol.Plant.93. 572-575 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato, S., Mikami, T., Shimamoto, Y.: "Nucleotide sequence and transcription of the gene for ATPase subunit 9 in apple plant (Malus *domestica Borkh.) . Acta" Horticulturae. 392. 235-241 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kubo,T.: "The chloroplast trnP-trnW-petG gene cluster in the mitochondrial genomes of Beta vulgaris,B.trigyna and B.webbiana:evolutionary aspects" Current Genetics. 27. 285-289 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kubo,T.: "Physical and gene organization of mitochondrial DNA from the fertile cytoplasm of sugarbeet(Beta vulgaris L.)" Current Genetics. 28. 235-241 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Senda,M.: "Mitochondrial gene variation and phylogenetic relationships in the genus Beta" Theoretical and Applied Genetics. 90. 914-919 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato,S.: "Mitochondrial DNA analysis reveals cytoplasmic variation within a single cultivar of perennial ryegrass(Lolium perenne L.)" Euphytica. 83. 205-208 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kato,S.: "The apple mitochondrial atp9 gene:RNA editing and co-transcription with exons a and b of the nad5 gene" Physiologia Plantarum. 93. 572-575 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kato,S.: "Nucleotide sequence and transcription of the gene coding for ATPase subunit 9 in apple plant(Malus × domestica Borkh.)" Acta Horticulturae. 392. 235-241 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kubo,T.: "The chloroplast trnP-trnW-petG gene cluster in the mitochondrial genoms of Beta vulgaris,B.trigyna and B.webbiana.evolutionary aspects." Current Genetics. 27. 285-289 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Senda,M.: "Mitochondrial gene variation and phylogenetic relationship in the genus Beta." Theoretical and Applied Genetics. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kato,S.: "Nucleotide sequence and transcription of the gene coding for ATPase subunit 9 in apple plant(Malus x domestica Borkh)." Acta Horticulturae. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kato,S.: "The apple mitochondrial atpy gene:RNA editing and co-transcription with exons a and b of the nad5 gene." Physiologia Plantarum. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kishima,Y.: "Chloroplast genomes in buckwheat and its related species;construction of the physical map and phylogenetic relationships." Plant Science. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kishima,Y.: "Physical mapping of differences between the chloroplast DNAs of Beta vulgaris and B.webbiana." Plant Breeding. (in press).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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