| Project/Area Number |
06454044
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Breeding science
|
|---|
| Research Institution | KYOTO UNIVERSITY |
|---|
Principal Investigator |
OKUMOTO Yutaka Kyoto Univ., Fac.of Agric., Lecturer, 農学部, 講師 (90152438)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
INOUE Hiromo Kyoto Univ., Fac.of Agric., Instructor, 農学部・附属農場, 助手 (40260616)
NAKAZAKI Tetsuya Kyoto Univ., Fac.of Agric., Instructor, 農学部, 助手 (60217693)
TANISAKA Takatoshi Kyoto Univ., Fac.of Agric., Associate Professor, 農学部, 助教授 (80026591)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1994: ¥4,000,000 (Direct Cost: ¥4,000,000)
|
|---|
| Keywords | mutator / RLGS / YAC / mapping / rice / YAC / RFLP / RAPD |
|---|
| Research Abstract |
The construction of a fine molecular map for slg locus that activates the mutator in rice was carried out. First, YAC clones containing slg locus was surveyed using the YAC library provided form the Rice Genome Team of the Ministry of Agricultural Forestry and Fishery. Second, DNA markers closely linked to slg locus were surveyed through a RFLP analysis and a Restriction Landmark Genomic Scanning (RLGS). Third, the existence of retro transposon-like elements in slg locus was surveyed through a Southern-blot analysis using retro transposons as DNA probes. The survey of YAC clones showed that a YAC clone Y4270 contained RFLP locus G33 linked with slg locus (recombination value 3%). Both ends of the Y4270 were amplified through inverse PCR,then a Southern-blot analysis was carried out using these amplified DNAs as the probes The experimental results showed that both ends of the Y4270 linked to slg locus. From this result, it could be said that one of the two YAC ends should be located much closer than G33. With the use of the RLGS,the comparison was made between the original variety of Gimbozu and the mutant line (homogeneous for slg). As a result of the RLGS analysis, three DNA spots were found specific to either Gimbozu or the mutant line. However, no relation was found between the segregation of the polymorphism and the segregation of slg locus. DNA polymorphism related with slg locus was found when Tos17, one of the retro transposon, was used as the probe. Consequently, the accurate recombination values between above DNA segments and slg locus should be clarified to construct the fine molecular map for slg locus with the use of much larger mapping population.
|
