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Study of the defense response and gene regulation for pathogen attack

Research Project

Project/Area Number 06454063
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

YAMADA Tetsuji  OKAYAMA UNIVERSITY Faculty of Agriculture PROFESSOR, 農学部, 教授 (00191320)

Co-Investigator(Kenkyū-buntansha) ICHINOSE Yuki  OKAYAMA UNIVERSITY Faculty of Agriculture Assistant Professor, 農学部, 助教授 (50213004)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1995: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1994: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywordsphenylalanine ammonia-lyase / PAL / elicitor / pathogen ingress / GUS / PSPAL1 / PSPAL2 / フェニールアラニン・アンモニア・リアーゼ / UV / 菌感染 / カルコン合成酵素 / CHS / サプレッサー / エンドウ褐紋病菌 / シス・トランス作動因子
Research Abstract

The object of this research is to elucidate the mechanisms of signal transduction and control of the genes involved in plant active defense responses conferring host-pathogen specificity. We intensively characterized the molecular basis involved in the activation and deactivation of plant active defense genes encoding PAL and CHS in pea, particularly focused on the study of the effects of elicitor and suppressor produced by a pea pathogen, Mycosphaerella pinodes. Details of the current status of the studies are,
1.Artificial reconstruction of a members of PAL gene (PSPAL1) and in a small multigene family of pea demonstrated that the conserved sequence motifs of Box 2 and Box 4 were very important in the activation mediated by the treatment with fungal elicitor by so called Loss of Function and Gain of Function Experiments. However, Box 1 functions in a concerted manner with Box 2 and Box 4, probably forming a transcriptional activation complex.
2.Generation of transgenic tobacco carrying the chimeric PSPAL1 promoter-GUS-Nos terminator construct (same types of the chimeric gene as described in 1 except these construct are connected to GUS reporter gene instead of CAT gene) demonstrated that Boxes1,2, and 4, are prerequisite cis-elements in response to the pathogen attack. These elements are also important in response to the activation mediated by the treatment with fungal elicitor. -1,394 to +140 or to +115 of PSPAL1 (translational fusion type) promoter contributed the tissue-specific expression, particularly in the merismatic tissues of roots, lower part of stems and very high expression in the tissues of flower organs where pink pigment is deposited and lignins are vigorously for med. Chimeric fusion of -1,394 to +5 or to -27 exhibited very little GUS expression, indicating that the exonic sequences are important in the expression if transgenic tobacco.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (63 results)

All Other

All Publications (63 results)

  • [Publications] Kato, H. et al.: "Characterization of nuclear factors for elicitor-mediated activation of the promoter of the pea phenylalanine ammonia-lyase gene 1." Plant Physiology. 108. 129-139 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wada, M., et al.: "Supprescion from a phytopathogenic fungus deactivates transcription of a plant defense gene encoding phenylalanine ammonia-lyase." J. Mol. Biol.249. 513-519 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tan, S. H. et al.: "Establishment of a system for the transient expression of GUS gene in electroporated muskmelon (Cucumis melo L.) protoplasts." Asia Pacific Journal of Molecular Biology and Biotechnology. 3. 156-159 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Saleh, N. H. et al.: "Expression of heterologous pa' gene in Muskmelon (Cucumis melo L. Birdie)." Asia Pacific Journal of Molecular Biology and Biotechnology. 3. 206-214 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiramoto, T. et al.: "The relationship between systemic resistance induced by pruning and accumulation of antifungal substances in barley seedling." J. Phytopath.143. 43-46 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiramoto, T. et al.: "Signal molecules inducing systemic resistance and susceptibility in barley seedling" J. Phytopath.143. 47-51 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyoda, K. et al.: "Plant lectins induce the production of a phytoalexin in Pisum sativum." Plant Cell Physiol.36. 799-807 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nasu, K. et al.: "Suppression of the activation of chitinase and β -1, 3-glucanase in peaepicotyls by endogenous suppressor from Pisum sativum." Ann. Phytopath. Soc. Japan. 61. 13-17 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiba, A. et al.: "Specific inhibition of cell wall-bound ATPase by fungal suppressor from Mycosphaerella pinodes." Plant. Cell Physiol.36. 809-817 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Amano, M. et al.: "H^+ -translocating activity in proteoliposomes reconstituted with pea plasma membrane ATPase and its inhibition by fungal suppressor from Mycosphaerella pinodes." Ann. Phytopathol. Soc. Japan. 61. 134-136 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiraishi, T, et al.: "Phenylalanine ammonia-lyase in barley : Activity enhancementin response to Erisiphe graminis f. sp. hordei (race 1) a pathogen, and Erisiphe pisi, a nonpathogen." Physiol. Mol. Plant Pathol.46. 153-162 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiraishi, T, et al.: "Regulation of plant defense responses by signals from fungal pathogens." 日本農芸化学会誌. 69. 1-177 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiraishi, T, et al.: "Suppressor produced by a pea pathogen, M. Pinodes." Grain Legumes. 4. 22-23 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiraishi, T, et al.: "Suppressors : Deteminants of specificity produced by plan pathogens." Plant Cell Phisiol.35. 1107-1119 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, T. et al.: "Suppression of pisatin and phenylalanine ammonia lyase-mRNA in a compatible interaction between Pisum sativum L. ev. Midoriusui and Pseudomonas syringae pv. pisi." Ann. Phytopath. Soc. Japan. 60. 66-73 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, T. et al.: "Functional analysis of the promoters of phenylalanine ammonia-lyase genes in pea." Plant Cell Physiol.35. 917-926 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 白石友紀・山田哲治: "植物感染を制御する病原菌シグナル" バイオサイエンスとインダストリー. 53. 16-21 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 山田哲治・白石友紀: "バイオテクノロジーによる植物の病害抵抗性機構の解明とアグリメデイカル的有用植物の創造" New Food Industry. 37. 65-71 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, H. et al.: "Analysis of cis-regulatory elements involved in the activation of a member of chalcone synthase gene family (PsCHS1) in pea." Plant Mol. Biol.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiraishi, T.et al.: "Suppressor produced by a pea pathogen, M.pinodes." Grain Legumes. 4. 22-23 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiraishi, T.et al.: "Suppressors : Determinants of specificity produced by plant pathogens." Plant Cell Phisiol.35-8. 1107-1119 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, T.et al.: "Suppression of pisatin and phenylalanine ammonia lyase-mRNA in a compatible interaction between Pisum sativum L.cv.Midoriusui and Pseudomonas syringae pv.pisi." Ann.Phytopath.Soc.Japan. 60. 66-73 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, T.et al.: "Functional analysis of the promoters of phenylalanine ammonia-lyase genes in pea." Plant Cell Physiol.35-6. 917-926 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato, H.et al.: "Characterization of nuclear factors for elicitor-mediated activation of the promoter of the pea phenylalanine ammonia-lyase gene 1." Plant Physiology. 108. 129-139 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wada, M., et al.: "Supprescin from a phytopathogenic fungus deactivates transcription of a plant defense gene encoding phenylalanine ammonia-lyase." J.Mol.Biol.249. 513-519 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tan, S.H.et al.: "Establishment of a system for the transient expression of GUS gene in electroporated muskmelon (Cucumis melo L.) protoplasts." Asia Pacific Journal of Molecular Biology and Biotechnology. 3-2. 156-159 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Saleh, N.H.et al.: "Expression of heterologous pal gene in Muskmelon (Cucumis melo L.Birdie)." Asia Pacific Journal of Molecular Biology and Biotechnology. 3-3. 206-214 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyoda, K.et al.: "Plant lectins induce the production of a phytoalexin in Pisum sativum." Plant Cell Physiol.36. 799-807 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiramoto, T.et.al.: "The relationship between systemic resistance induced by pruning and accumulation of antifungal substances in barley seedling." J.Phytopath.143. 43-46 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiramoto, T.et al.: "Signal molecules inducing systemic resistance and susceptibility in barley seeding." J.Phytopath.143. 47-51 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nasu, K.et al.: "Supprescin of the activation of chitinase and beta-1,3-glucanase in pea epicotyls by endogenous suppressor from Pisum sativum." Ann.Phytopath.Soc.Japan. 61-1. 13-17 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiba, A.et al.: "Specific inhibition of cell wall-bound ATPase by fungal suppressor from Mycosphaerella pinodes." Plant Cell Phisiol.36. 809-817 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Amano, M.et al.: "H^+ -translocating activity in proteoliposomes reconstituted with pea plasma membrane ATPase and its inhibition by fungal suppressor from Mycosphaerella pinodes." Ann.Phytopathol.Soc.Japan. 61-4. 134-136 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiraishi, T.et al.: "Phenylalanine ammonia-lyase in barley : Activity enhancement in response to Erisiphe graminis f.sp.hordei (race 1) a pathogen, and Erisiphe pisi, a non pathogen." Physiol.Mol.Plant Pathol.46. 153-162 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiraishi, T.et al.: "Regulation of plant defense responses by signals from fungal pathogens." Nippon Nogeikagaku Kaishi. 69-2. 174-177 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, H.et al.: "Analysis of cis-regulatory elements involved in the activation of a member of chalcone synthase gene family (PsCHS1) in pea." Plant Mol.Biol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato,H.et al.: "Characterization of nuclear factors for elicitor-mediated activation of the promoter of the pea phenylalanine ammonia-lyase gene 1." Plant Physiology. 108. 129-139 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wada,M.,et al.: "Supprescin from a phytopathogenic fungus deacti vates trans cription of a plant defense gene encoding phenylalanine ammonia-lysse." J.MoL Biol.249. 513-519 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toyoda,K.et al.: "Plant lectins induce the production of a phytoalexin in Pisum sativum." Plant Cell PhysioL. 36. 799-807 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiramoto,T.et al.: "The relationship between systemic resistance induced by pruning and accumulation of antifungal substances in barley seedling." J.Phytopath.143. 43-46 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiramoto,T.et al.: "Signal molecules inducing systemic resistance and susceptibility in barley seedling." J.Phytopath.143. 47-51 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nasu,K.et al.: "Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by endogenous suppressor from Pisum sativum." Ann,Phytopath.Soc.Japan. 61. 13-17 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kiba,A.et al: "Specific inhibition of cell wall-bound ATPase by fungal suppressor from Mycosphaerella pinodes." Plant Cell PhysioL. 36. 809-817 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Amano,M.et al.: "H^+-translocating activity in proteoliposomes reconstituted with pea plasma membrane ATPase and its inhibition by fungal suppressor from Mycosphaerella pinodes." Ann,Phytopathol.Soc.Japan. 61. 134-136 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shiraishi,T.et al.: "Phenylalanine ammonia-lyase in barley : Activity enhancementin response to Erisiphe graminis f.sp.hordei (rece 1) a pathogen,and Erisiphe pisi,a nonpathogen." Physiol.Mol.Plant Pathol.46. 153-162 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shiraishi,T.et al.: "Regulation of plant defense responses by signals from fungal pathogens." 日本農芸化学会誌. 69. 174-177 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 白石友紀・山田哲治: "植物感染を制御する病原菌シグナル" バイオサイエンスとインダストリー. 53. 16-21 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 山田哲治・白石友紀: "バイオテクノロジーによる植物の病害抵抗性機構の解明とアグリメデイカル的有用植物の創造" New Food Industry. 37. 65-71 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Saleh,N.H.et al.: "Expression of heterologous pal gene in Muskmelon (Cucumis meloL.Birdie)." Asia Pacific Journal of Molecular Biology and Biotechnology. 3. 206-214 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tan,S.H.et al.: "Establishment of a system for the transient expression of GUS gene in electroporated muskmelon (Cucumis melo L.) protoplasts." Asia Pacific Journal of Molecular Biology and Biotechnology. 3. 156-159 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Seki,H.et al.: "Analysis of cis-regulatory elements involved in the activation of a member of chalcone synthase gene family (PsCHS1) in pea." Plant Mol.Biol.in press.(1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 山田哲治他: "植物感染機構の進化を考える" 日本植物病理学会, 15 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 山田哲治: "植物病理学事典" 養賢堂, 7 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Shiraishi,et al.: "Suppressors:Determinants of specificity produced by plant pathogens." Plant Cell Phisiol.35. 1107-1119 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Kato,et al.: "Characterization of nuclear factors for elicitor-mediated activation of the promoter of the pea phenylalanine ammonia-lyase gene 1." Plant Physiol.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Hiramoto,et al.: "Signal molecules inducing systemic resistance and susceptibility in barley seedling." J.Phytopath.143(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Hiramoto,et al.: "The relationship between systemic resistance induced by pruning and accumulation of antifungal substances in barley seedling." J.Phytopath.143(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Nasu,et al.: "Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by endogenous suppressor from Pisum sativum." Ann.Phytopath.Soc.Japan. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Wada,et al.: "Supprescin from a phytopathogenic fungus deactivates transcription of a plant defense gene encoding phenylalanine ammonia-lyase." J.Mol.Biol.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 白石友紀: "病原菌シグナルと植物の防御応答" 日本農芸化学会誌. 69(印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Yamada,et al.: "Functional analysis of the promoters of phenylalanine ammonia-lyase genes in pea." Plant Cell Physiol.35. 917-926 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Shiraishi,et al.: "Phenylalanine ammonia-lyase in barley:Activity enhancement in response to Erysiphe graminis and Erysiphe pisi." Physiol.Mol.Plant Pathol.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 山田哲治: "植物病理学事典,「がん腫病」の項" 養賢堂(印刷中), (5) (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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