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Triala for rapid identification of phytopathogenic bacteria

Research Project

Project/Area Number 06454064
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionKyushu University

Principal Investigator

MATSUYAMA Nobuaki  Kyushu University, Agronomy, Professor, 農学部, 教授 (40108676)

Co-Investigator(Kenkyū-buntansha) FURUYA Naruto  Kyushu University, Agronomy, Assist. Professor, 農学部, 助手 (10211533)
TAKANAMI Yoichi  Kyushu University, Agronomy, Assoc. Professor, 農学部, 助教授 (50243944)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsSimplified Identification / Rapid Identification / Phytopathogenic Bacteria / Chromatography / HPLC / GLC / TLC
Research Abstract

The direct colony thin layr chromatography was firstly applied for rapid identification of phytopathogenic bacteria. The defferentiation of the phytopathogenic bacteria at generic level was available amongClavibacter, Agrobacterium and others. Further, in genus Erwinia, the profiles of E.chrysanthemi and E.carotovora subsp. carotovora were clearly different. These differences at apecies level were also observed in genus Pseudomonas. The results indicated practical usefulness of this easy method for rapid identification of phytopathogenic bacteria. During these experiments, the existence of several groups which had similarities for chromatographic profiles in pseudomonads was found. These groups were Cepacia-type, Syringae-type and Solanacearum-type.
Furthemore, the similarities and diversities of chromatographic profiles among the species of pseudomonads which were proposed to be transferred to a new genus Burkholderia were investigated.
The chromatograms of Pseudomonas cepacia, P.caryophylli, P.glumae, P.gladioli, P.plantarii P.andropogonis, P.solanacearum and P.rubrisubalbicans resembled roughly each other. However, the profiles of three spots which were desibnated as S1, S2 and S3 around Rf0.6 were distinct at species level. The chromatograms of P.andropogonis, P.solaceacesrum and P.rubrisubalbicans were characteristic. Especially, the profile of P.rubrisubalbicans was quite unique in these pseudomonads. So far as the chromatograms for amino-lipids is concerned, some similarities were observed between P.andoropogonis and P.solanacearm. However, P.andropogonis was distinguishable with the existence of single spot which is detectable at Rf0.83 by spraying of the chromium-containing 55% sulfuric acid and heating at 110゚C.The usefulness of the direct colony TLC method for rapid identification of phytopathogenic bacteria at species level was repeatedly verified.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Matsuyama, N.: "Trials for rapid identification of phytopathogenic bacteria by HPLC and the direct colony TLC" J. Fac. Agr. Kyushi Univ.40. 87-91 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuyama, N.: "Application of the direct colony TLC for identification of phytopathogenic bacteria (III). Distinction of pseudomonads of the rRNA homology group II (Burkholderia spp.)" J. Fac. Agr. Kyushu Univ.40. 189-196 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 塩崎尚美・井上美由紀・松元 賢・飯山和弘・古屋成人・松山宣明: "イネ褐条病細菌(Pseudomonas avenae)の分類学的検討" 九病虫研報. 41. 1-6 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 井上美由紀・松元 賢・古屋成人・松山宣明: "Pseudomons syringaeにおける全菌体及び菌体膜脂肪酸組成の比較" 九病虫研報. 41. 55-57 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuyama, M.: "Trials for rapid identification of phytopathogenic bacteria by HPLC and the direct colony TLC" J.Fac.Agr.Kyushu Univ.40(1,2). 87-91 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuyama, M.: "Application of the ditect colony TLC for identification of phytopathogenic bacteria (III).Distinction of the pseudomonads in the rRNA-homology group II (Burkholderia spp.)" J.Fac.Agr.Kyushu Univ.40(1,2). 189-196 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiozaki, M., Inoue, M., Matsumoto, M., Iiyama, K., Furuya, N.and Matsuyama, N.: "Studies on the assignment of taxonomic status of Pseudomonas avenae" Proc.Assoc., Pl.Prot.Kyushu. 41. 1-6 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Inoue, M., Matsumoto, M., Furuya, N.and Matsuyama, N.: "Comparative studies on fatty acid composition of the membrane and whole cells of Pseudomonas syringae" Proc.Assoc., Pl.Prot.Kyushu. 41. 55-57 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuyama, N.: "Trials for rapid identification of phytopathogenic bacteria by HPLC and the direct colony TLC" J. Fac. Agr. Kyushu Univ.40. 87-91 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsuyama, N.: "Application of the direct colony TLC for identification of phytopathogenic bacteria(III)." J. Fac. Agr. Kyushu Univ.40. 189-196 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 井上美由紀: "Pseudomonas syringaeにおける全菌体及び菌体膜脂肪酸組成の比較" 九病虫研報. 41. 55-57 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 塩崎尚美: "イネ褐条病細菌(Pseudomonas avenae)の分類学的検討" 九病虫研報. 41. 1-6 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 井上美由紀,他: "Pseudomonas syringaeにおける全菌体および菌体膜脂肪酸組成の比較" 九州病害虫研究会報. 40. (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 塩崎尚美,他: "イネ褐条病細菌(Pseudomonas avenae)の分類学的検討" 九州病害虫研究会報. 40. (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小野嘉子,他: "糸状菌の簡易同定法に関する研究(1)全菌体脂肪酸分析" 九州病害虫研究会報. 40. (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 松山宣明・古屋成人: "HPLCによる植物病原細菌迅速同定の試み(1)逆相系カラムの利用" 日本植物病理学会報. 61(3). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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