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Basic studies on the development of a low-cost artificial diet for the silkworms and the establishment of a year-round sericulture system

Research Project

Project/Area Number 06454067
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 蚕糸・昆虫利用学
Research InstitutionKyoto Institute of Technology

Principal Investigator

MATSUBARA Fujiyoshi  Kyoto Institute of Technology, Textile Science, Professor, 繊維学部, 教授 (50027877)

Co-Investigator(Kenkyū-buntansha) MORI Hajime  Kyoto Institute of Technology, Textile Science, Assistant Professor, 繊維学部, 助手 (80201812)
SUMIDA Motoyuki  Kyoto Institute of Technology, Textile Science, Associate Professor, 繊維学部, 助教授 (50127164)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsAn artificial diet / Aseptic rearing of silkworms / Three times feeding method throughout the larval instars / A year-round sericulture / Sericulture plant / Cocoon and raw silk characteristics / Production cost / Management / 低コスト / オカラ含有飼料 / 全令3回育 / 飼料成型 / 無菌蚕 / 省力生産法
Research Abstract

The aim of the present study is to establish a year-round sericulture system to carry out a year-round sericulture in Japan, to produce high quality cocoons throughout the year, thereby to activate the sericultural industry in Japan.
A new artificial diet was developed which included bean-curd lees, an industrial waste, in place of defatted soy bean powder as much as 50% for the grown silkworm larvae. A new preparation method of mulberry leaf powder with low facility cost, i.e., by drying mulberry leaves in the sun was developed. The required amount of dried mulberry leaf powder, other major raw materials and minor additives such as vitamins and minerals for the preparation of a low-cost artificial diet were established. Two types of a standard table for aseptically rearing silkworms, one for parent silkworm for hybridization, and another for hybrid races, on an artificial diet by twice or three times feedings throughout the larval stages was established. A standard expected results fro … More m a year-round sericulture system were established such as silkworm growth, pupation rate, adult emergence rate, amount of oviposition.
Facilities needed for a year-round sericulture system were investigated for two cases. One is to exploit the facilities now in use. The other is to make completely new facilities. A germ-free room and germ-free facilities were evaluated. Machineries for the preparation of an artificial diet was investigated and a new facilities were devised to make a considerable reduction of required labor.
A year-round sericulture system to rear silkworms twice a month, and total 24 times per year by the three times feeding method yielded the following results. Required days for larval growth, 24-25 days. Amount of harvested cocoons per one egg case, 32-35kg. Reelability percentage, 60-85%. Raw silk percentage of cocoon, 14.5-16.5%. Length of cocoon filament, 1000-1150m. Size of cocoon filament, approximately 2.2-2.6 deniers. No tendency to become yellowish. Lustrous characteristics. Items of labor and required time for each item of labor, required expenses and production cost were, for the first time, calculated for the year-round sericulture system of three times feedings method throughout the larval instars. Profitability of a plant to produce 100 tons of cocoons per year by using the presently proposed system was evaluated. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] 松原藤好: "人工飼料無菌飼育法をベースにした我が国の新しい周年養蚕に関する研究II、周年無菌養蚕の施設及び設備" 京都工芸繊維大学繊維学部 学術報告. 19. 25-36 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 松原藤好: "無菌環境の特異性,蚕糸生産の立場から" 日本無菌生物学会誌. 25. 15-17 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 今村利勝: "無菌周年用蚕種の人工飼料無菌飼育による周年生産" 日本無菌生物学会誌. 25. 40-42 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 今村利勝: "無菌飼育法を導入した周年養蚕-特に工薬的生産方法を導入した場合の無菌飼育施設および設備" 日本無菌生物学会誌. 25. 46-49 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 森肇: "無菌カイコを宿主としたバキュロウイルスベクターによる外来遺伝子発現系" 日本無菌生物学会誌. 25. 50-52 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 角田素行: "全令3回給餌法による原蚕の飼育成績及び産卵成績" 日本蚕糸学雑誌. 64. 35-38 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 松原藤好: "新版生物環境調節ハンドブック" 養覧堂, 585(484-492) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 松原藤好: "生物実験の手引:(実験動物としての無菌蚕)" 京都府生物教育会, 237(17-19) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mori H., Tawara H., Nakazawa H., Sumida M., Matsubara F., Aoyama S., Hayashi Y.and Kamogawa K.: "Expression of the Newcastle disease virus (NDV) fusion glycoprotein and vaccination against NDV challenge with a recombinant baculovirus." Avian Diseases. 38. 772-777 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sumida M., Yuan X.L.and Matsubara F.: "Purification and some properties of soluble beta-fructofuranosidase from larval midgut of the silkworm, Bombyx mori." Comp.Biochem.Physiol.108B. 273-284 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsubara F., Sumida M., Mori H., Chen R.and Imamura T.: "A new industrial system of sericulture in Japan : year-round production of cocoons, based on aseptic rearing system of silkworms on an artificial diet l." Development of low-cost artificial diet.Bull.Fac.Textile Sci., Kyoto Inst.of Technol.18. 49-69 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kotani E., Imamoto S., Tashiro M., Mori H., Sumida M., Matsubara F., Taniai K., Okuda-Kadono K., Kato Y., Yamakawa M.and Mori H.: "Cloning and expression of the gene of hemocytin, an insect humoral lectin which is homologous with the mammalian von Willebrand factor" Biochim.Biophys.Acta. 1260. 245-258 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sumida M., Yuhki T., Chen R., Mori H., Imamura T.and Matsubara F.: "Aseptic rearing of original silkworm strains on an artificial diet throughout the entire larval instars" J.Seric.Sci.Jpn.64 (1). 35-38 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Imamura T., Chen R., Zhang Y., Yuhki T., Mori H., Sumida M.and Matsubara F.: "Establishment of standard rearing conditions for aseptic rearing of original silkworm strains on an artificial diet throughout the entire larval instars." J.Seric.Sci.Jpn.64 (2). 173-178 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsubara F., Sumida M., Mori H., Imamura T.and Itagaki T.: "Study of a new year-round sericulture system incorporating aseptic rearing of silkworms on an artificial diet II.Facilities needed for year-round aseptic sericulture." Bull.Fac.Textile Sci., Kyoto Inst.of Technol.19. 25-36 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mori H., Yamao M., Nakazawa H., Sugahara Y., shirai N., Matsubara F., Sumida M.and Imamura T.: "Tranovarian transmission of a foreign gene in the silkworm, Bombyx mori, by Autographa californica nuclear polyhedrosis virus." Bio/Technology. 13. 1005-1007

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sumida M., Yoshio H., Tanaka Y.and Matsubara F.: "Developmental changes in urea concentrations in the haemolymph of Daizo (T) , an original strain of silkworm, Bombyx mori reared on an artificial diet and on fresh mulberry leaves." Comp.Biochem.Physiol.110A. 33-38 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsubara F., Sumida M., Mori H.and Imamura T.: "A new year-round sericulture system incorporating aseptic rearing technique of silkworms on an artirificial diet III.Cost accounting of a factory scale system of year-round sericulture." Bull.Fac.Textile Sci., Kyoto Inst.of Technol.20. 27-36 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 松原藤好: "人工飼料無菌飼育法をベースにした我が国の新しい周年養蚕に関する研究II、周年養蚕の施設及び設備" 京都工芸繊維大学繊維学部学術報告. 19. 25-36 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 松原藤好: "無菌環境の特異性:蚕糸生産の立場から" 日本無菌生物学会誌. 25. 15-17 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 今村利勝: "無菌周年用蚕種の人工飼料無菌飼育による周年生産" 日本無菌生物学会誌. 25. 40-42 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 今村利勝: "無菌飼育法を導入した周年養蚕一特に工業的生産法を導入した場合の無菌飼育施設および設備" 日本無菌生物学会誌. 25. 46-49 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 森肇: "無菌蚕を寝主としたバキュロウイルスベクターによる外来遺伝子発現系" 日本無菌生物学会誌. 25. 50-52 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 角田素行: "全令3回給餌法による原蚕の飼育成績及び産卵成績" 日本蚕糸学雑誌. 64. 25-38 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 松原藤好: "新版生物環境調節ハンドブック" 養賢堂, 585 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 松原藤好: "生物実験の手引" 京都府生物教育会, 237 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 今村利勝: "人工飼料無菌飼育による周年養蚕" 無菌生物. 24. 69-70 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 松原藤好: "1-4令人工飼料無菌飼育・5令条桑育による養蚕法に関する研究" 無菌生物. 24. 74-79 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 角田素行: "全令3回給餌法による原蚕の飼育成績および産卵成績" 日本蚕糸学雑誌. 64. 35-38 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 松原藤好: "人工飼料無菌飼育法をベースにした我が国の新しい周年養蚕に関する研究" 京都工芸繊維大学繊維学部学術報告. 19. 25-36 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 松原藤好: "「無菌環境の特異性」蚕糸生産の立場から" 日本無菌生物ノートバイオロジー学会. 11 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 今村利勝: "無菌周年養蚕用蚕種の人工飼料無菌飼育による周年生産" 日本無菌生物ノートバイオロジー学会(講要集). 16 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 松原藤好: "真気技術(ノートバイオロジーを導入した世界で始めての周年養蚕" (株)ゼオクト, 1-22 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 松原藤好: "生物環境調節ハンドブック(養蚕施設)" 養賢堂, 184-493 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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