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Molecular physiology on ion transport sytems in silkworms

Research Project

Project/Area Number 06454069
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 蚕糸・昆虫利用学
Research InstitutionTottori University

Principal Investigator

AZUMA Masaaki  Tottori Univ.Faculty of Agric.Associate Prof., 農学部, 助教授 (20175871)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Masahiko  Tokyo Univ.Faculty of Agric.Professor, 農学部, 教授 (60162020)
KOBARA Ryuzo  Tottori Univ.Faculty of Agric.Professor, 農学部, 教授 (70032092)
KAI Hidenori  Tottori Univ.Faculty of Agric.Professor, 農学部, 教授 (60023412)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordssilkworm / midgut / amino acid transport / V-ATPase / Potassium pump / Proton pump / silk gland / Bombyx mori
Research Abstract

Insect midgut in Lepidoptera (the main part of the alimentary canal) is a central and indispensable tissue to exchange various ionic solutes across the epithelia and to maintain the cellular activity and the tissue functions (s). We have investigated such a mechanism to understand the ionic homeostasis and osmoregulation in the silkworm system. Results ar as follows :
(1) Immunocytochemical studies were done with the specific anitibody against V-ATPase. In silkwrms, V-ATPase can be detected in the midgut, the Malpighian tubules, the salivary gland and the silk gland. V-ATPase in each tissue was located at the cell surface, suggesting that silkworm V-ATPase is a plasma membrane-type not an endomenbrane-type.
(2) In the silk gland, V-ATPase seems to contribute to produce an acidic milieu in the glandular lumen. This shows highly contrast to the midgut lumen, which produce an extremely high alkalinity (pH 11-12).
(3) The silk gland V-ATPase is quite similar to other animal V-ATPases in the common subunits of V-ATPase.
(4) K+/amino acid symporter in the midgut was rather labile protein and hard to purify. Its molecular nature still remains unknown.
(5) The silk gland is highly active tissue for silk protein syntesis and secretion. This implies that the tissue needs much supply of amino acids from the haemolymph to synthesize the silk protein. Thus, it is coming up as the quite near future subject to survey the K+/amino acid symporter in the silk gland. This would be expected as the breakthrough for identifing the K+/symporter molecule (s).

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Lepier, A: "K^+/H^+ antiport in the tobacco hornworm midgut : the K^+-transporting component of the K^+ pump" The Journal of Experimental Biology. 196. 361-373 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 東 政明: "鱗翅目昆虫中腸の構造とその生理機能-最近の進歩-" 日本蚕糸学雑誌. 64. 1-18 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Azuma, M.: "Stoichiometry of K^+/H^+ antiport helps to explain extracellular pH 11 in a model epithelium" FEBS letters. 361. 153-156 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Lepier, A.: "The peripheral complex of the tobacco hornworm V-ATpase contains a novel 13-kDa suburit G" Journal of Biological Chemistry. 271. 8502-8508 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 東 政明: "カイコの前部絹糸線細胞における液胞型H^+輸送性ATPase(V-ATPase)の存在とその腺腔酸性化機能" 日本蚕糸学雑誌. 65. 303-311 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 東 政明: "消化管とその機能。“昆虫学-基礎と応用-"(分担執筆),三橋 淳ら編著,朝倉書店,東京。" (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Lepier, A.: "K^+/H^+ antiport in the tobacco hornworm midgut : the K^+-transporting component of the K^+ pump" J.Exp.Biol.196. 361-373 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Azuma, M.: "Recent advances in lepidopteran midgut functions (in Japanese)" J.Seric.Sci.Jpn.64. 1-18 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Azuma, M.: "Stoichiometry of K^+/H^+ antiport helps to explain extracellular pH 11 in a model epithelium" FEBS Letters. 361. 153-156 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Lepier, A.: "The peripheral complex of the tabacco hornworm V-ATPase contains a novel 13-kDa subunit G" J.Biol.Chem.271. 8502-8508 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Azuma, M.: "Acidification of the glandular lumen by a vacuolar-type H^+ATPase in the anterior silk gland cell of Bombyx mori (in Japanese with English summary)" J.Seric.Sci.Jpn.65. 303-311 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Azuma, M.: Asakura Press, Tokyo (in press). Insect gut -its cellular functions (In Japanese) In "Entomology - fundamentals and its application-" (eds.J.Mitsuhashi et al.),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Lepier,A.: "K^+/H^+ antiport in the tobacco hornworm midgut: the K^+-transporting component of the K^+ pump" The Journal of Experimental Biology. 196. 361-373 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] 東 政明: "鱗翅目昆虫中腸の構造とその生理機能-最近の進歩-" 日本蚕糸学雑誌. 64. 1-18 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Azuma,M.: "Stoichiometry of K^+/H^+ antiport helps to explain extracellular pH 11 in a model rpithellum" FEBS letters. 361. 153-156 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Lipier,A.: "The peripheral complex of the tobacco hornworm V-ATPase contains a novel 13-kDa subunit G" Journal of Biological Chemistry. 271. 8502-8508 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 東 政明: "カイコ前部絹糸線細胞における液胞型H^+輸送性ATPase(V-ATPase)の存在とその腺腔酸性化機能" 日本蚕糸学雑誌. 65. 303-311 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 東 政明: "消化管とその機能。“昆虫学-基礎と応用-"(分担執筆),三橋 淳ら編著,朝倉書店,東京。" (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Lepier,A.: "K^+/H^+ antiport in the tobacco hormworm midgut : the K^+-transporting component of the K^+ pump" The Journal of Experimental Biology. 196. 361-373 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 東 政明: "鱗翅目昆虫中腸の構造とその生理機能-最近の進歩-" 日本蚕糸学雑誌. 64. 1-18 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Azuma,M.: "Stoichiometry of K^+/H^+ antiport helps to explain extrancellular pH 11 in a model epithelium" FEBS letters. 361. 153-156 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Lepier,A.: "The periheral complex of the tobacco hornworm V-ATPase contains a novel 13-kDa subunit G" Journal of Biological Chemistry. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 東 政明: "カイコ前部絹糸線細胞における液胞型H^+輸送性ATPase(V-ATPase)の存在とその腺腔酸性化機能" 日本蚕糸学雑誌. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Lepier,A.: "K^+/H^+ antiport in the tobacco hornworm midgut:the K^+-transporting component of the K^+ pump" The Journal of Experimental Biology. 196. 361-373 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 東 政明: "鱗翅目昆虫中腸の構造とその生理機能-最近の進歩-" 日本蚕糸学雑誌. 64. 1-18 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Azuma,M.: "Stoichiometry of K^+/H^+ antiport helps to explain extracellular pH 11 in a model epithelium" FEBS letters. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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