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Regulation of secondary metabolism and cell differentiation by A-factor and protein phosphorylation in Streptomyces.

Research Project

Project/Area Number 06454073
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・応用生物化学
Research InstitutionThe University of Tokyo

Principal Investigator

HORINOUCHI Sueharu  University of Tokyo, Biotechnology, Professor, 農学部, 教授 (80143410)

Co-Investigator(Kenkyū-buntansha) NISHIYAMA Makoto  University of Tokyo, Biotechnology Center, Assoc.Prof., 生物生産工学研究センター, 助教授 (00208240)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥7,100,000 (Direct Cost: ¥7,100,000)
KeywordsStreptomycetes / Utoregulator / Antibiotic / Morphogenesis / Signal transduction / A-ファクター / 抗生物質生産
Research Abstract

(1) The A-factor recepor protein (ArpA) was purified from S.griseus and arpA was cloned by PCR.We also cloned two arpA homologs from S.coelicolor A3 (2), which probably controlled antibiotic production in this strain, as determined by the phenotypes of each of disruptants.
(2) Transcription of amfR,a regulatory gene for aerial mycelium formation in S.griseus, was controlled by A-factor. A protein able to bind to its promoter region was purified and its gene was cloned.
(3) An afsK homolog (afsK2) was cloned from S.griseus. Genetic studies showed that the afsK2 gene is involved in aerial mycelium formation under the conditons of high osmolality
(4) Upstream from afsA was present a gene encoding a protein similar to prokaryotic transcriptional factors. The expression of afsA therefore apeared to be controlled by this upstream gene.
(5) An A-factor-responsive transcriptional activator (Adp) that bindsto the strR promoter was purified to homogeneity. The molecular mass of Adp was 40 kDa.
(6) A tyrosine phosphatase gene was cloned from S.coelicolor A3 (2) as a gene that caused pigmentation in S.lividans. The phosphatase produced in E.coli showed a tyrosine phosphatase activity.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] S.Horinouchi: "A-factor as a microbial hormone that controls cellular differentiation and secondary metabolism in S. griseus" Molecular Microbiology. 12. 859-864 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Kudo: "Plasmid-mediated gene disruption in Streptomyces griseus" Actinomycetologica. 8. 17-20 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Miyake: "Organization and nucleotide sequence of the secE-musG region of Streptomyces griseus" Biochimica et Biophysica Acta. 1217. 97-100 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Matsumoto: "Phosphorylation of the AfsR protein involved in secondary metabolism in Streptomyces spp. by a eukaryotic-type proteinkinase" Gene. 146. 47-56 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Horinouchi: "Streptomyces species as boundary microorganisms : eucaryotic regulatory systems for secondary metabolism and morphological differentiation" Actinomycetologica. 9. 244-253 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Kudo: "Cloning and characterization of a gene involved in aerial mycelium formation in Streptomyces griseus" Journal of Bacteriology. 177. 6401-6410 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Matsumoto: "Involvement of a small ORF downstream of the afsR gene in the regulation of secondary metabolism in S. coelicolor" Actinomycetologica. 9. 37-43 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Onaka: "Cloning and characterization of the A-factor receptor gene from streptomyces griseus" Journal of Bacteriology. 177. 6083-6092 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Ueda: "Overexpression of a gene encoding a chalcone-aynthase-like gene confers redbrown pigment production in S. griseus" Journal of Antibiotics. 48. 638-646 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Ueda: "The aerial mycelium-defective phenotype of &. griseus resulting from A-factar deficiency is suppressed by a Ser/Thr kinase of S. coelicolor" Gene. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Horinouchi: "A hormonal control system in Streptomyces : A-factor and its specific regulator" Archieves of Microbiology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 堀之内末治: "放線菌の抗生物質生産・形態分化におけるシグナル伝達" 蛋白質核酸酵素. 40. 986-999 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Horinouchi: "Genetics and Biochemistry of Antibiotic Production" Butterworth-Heinemann, Boston, 103-119 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Horinouchi: "Proc. of Int. Symp. on Functional Biomaterials" Korean Soc. Microbiology, 88-98 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Horinouchi: "Dynamic Aspect of Natural Products chemistry-Molecular Biological Approaches" Kodansha-Scientific(印刷中),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Horinouchi, S.: ""Eucaryotic" signal transduction systems involved in secondary metabolism and morphogenesis in the bacterial genus Streptomyces." World Scientific Publishing Co., Microbiolgy in the Nineties. Singapore. 43-48 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Horinouchi, S.: "Signal transduction via A-factor that controls antibiotic production and morphogenesis in Streptomyces." Proceedings of International Symposium on Functional Biomaterials. Seoul, Korea.88-98 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Horinouchi, S., and T.Beppu: "Autoregulators. Genetics and Biochemistry of Antibiotic Production. Butterworth-Heinemann Publishers, Boston" 103-119 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Horinouchi, S., and T.Beppu: "A-factor as a microbial hormone that controls cellular differentiation and secondary metabolism in Streptomyces griseus" Mol.Microbiol. 12. 859-864 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Horinouchi, S., T.Umeyama, H.Onaka, K.Ueda, and T.Beppu.: "Streptomyces species as boundary microorgaisms : eucaryotic regulatory systems for secondary metabolism and morphological differentiation." Actinomycetol. 9. 244-253 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kudo, N., M.Kimura, T.Beppu, and S.Horinouchi: "Cloning and characterization of a gene involved in aerial mycelium formation in Streptomyces griseus" J.Bacteriol. 177. 6401-6410 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kudo, N., K.Ueda, H.Ikeda, S.Omura, T.Beppu, and S.Horinouchi: "Pasmid-mediated gene disruption in Streptomyces griseus" Actinomycetol. 8. 17-20 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsumoto, A., S.-K.Hong, H.Ishizuka, S.Horinouchi, and T.Beppu.: "Phosphorylation of the AfsR protein involved in secondary metabolism in Streptomyces species by a eukaryotic-type protein kinase." Gene. 146. 47-56 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsumoto, A., H.Ishizuka, T.Beppu, and S.Horinouchi: "Inolvement of a small ORF downstream of the afsR gene in the regulation of secondary metabolism in Streptomyces coelicolor A3 (2)." Actinomycetol. 9. 37-43 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyake, K., H.Onaka, S.Horinouchi, and T.Beppu: "Organization and nucleotide sequence of the secE-nusG region of Streptomyces griseus." Biochim.Biophys.Acta. 1217. 97-100 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Onaka, H., N.Ando, T.Nihira, Y.Yamada, T.Beppu, and S.Horinouchi: "Cloning and characterization of the A-factor receptor gene Streptomyces griseus" J.Bacteriol. 177. 6083-6092 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ueda, K., K.-M.Kim, T.Beppu, and S.Horinouchi.: "Overexpression of a gene cluster encoding achalcone synthase-like protein confers redbrown pigment production in Streptomyces griseus." J.Antibiot.48. 638-646 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ueda, K., T.Umeyama, T.Beppu, and S.Horinouchi: "The aerial mycelium-defective phenotype of Streptomyces girseus resulting from A-factor deficiency is suppressed by a Ser/Thr kinase of S.coelicolor A3 (2)" Gene. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Horinouchi, S.: "A hormonal control system in Streptomyces : A-factor and its specific receptor." Arch.Microbiol. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sueharu Horinouchi: "Signal transduction via A-factor that controls antibiotic production and morphogenesis in Streptomyces." Proceedings of International Symposium on Functional Biomaterials. 88-98 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sueharu Horinouchi: "Streptomyces species as boundary microorganisms: eucaryotic regulatory systems for secondary metabolism and morphological differentiation." Actinomycetol.9. 244-253 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nobuaki Kudo: "Cloning and characterization of a gene involved in aerial mycelium formation in Streptomyces griseus." J.Bacteriol.177. 6401-6410 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Atsushi Matsumoto: "Involvement of a small ORF downstream of the afsR gene in the regulation of secondary metabolism in Streptomyces coelicolor A3(2)." Actinomycetol.9. 37-43 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroyasu Onaka: "Cloning and characterization of the A-factor receptor gene from Streptomyces griseus." J.Bacteriol.177. 6083-6092 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kenji Ueda: "Overexpression of a gene cluster encoding a chalcone synthase-like protein confers redbrown pigment production in Streptomyces griseus." J.Antibiot.48. 638-646 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kenji Ueda: "The aerial mycelium-defective phenotype of Streptomyces griseus resulting from A-factor deficiency is suppressed by a Ser/Thr kinase of S.coelicolor A3(2)." Gene. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sueharu Horinouchi: "A hormonal control system in Streptomyces: A-facter and its specific receptor." Arch.Microbiol.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 堀之内末治: "放線菌の抗生物質生産・形態分化におけるシグナル伝達" 蛋白質核酸酵素.

    • Related Report
      1995 Annual Research Report
  • [Publications] 堀之内末治: "微生物におけるホルモン制御:新しい抗生物質開発の可能性を拡大する放線菌のホルモン制御メカニズム、" 日経サイエンス. 25. 94- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sueharu Horinouchi: "Dynamic Aspects natural Products Chemistry-Molecular Biological Approaches" Kodansha-Scientific Co.(印刷中), (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Horinouchi: "A-factor as a microbial hormone that controls cellular differentiation and secondary metabolism in Streptomyces griseus" Mol.Microbiol.12. 859-864 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Kudo: "Plasmid-mediated gene disruption in Streptomycves griseus" Actinomycetol.8. 17-20 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Miyake: "Organization and nucleotide sequence of the secE-nusG reqion of Streptomyces qriseus" Biochim.Biophys.Acta. 1217. 97-100 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Horinouchi: "Genetics and Biocvhemistry of Antibiotic Production" Butterworth-Heinemann Publishers,Boston, 103-119 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Horinouchi: "Microbiology in the Nineties" World Scientific Publishing,Co.,Singapore, 43-48 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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