| Project/Area Number |
06454100
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | TOKYO UNIVERSITY OF FISHERIES |
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Principal Investigator |
HASOBE Masahide TOKYO UNIVERSITY OF FISHERIES.DEPARTMENT OF AQUATIC BIOSCIENCES,ASSOCIATED PROFESSOR, 水産学部, 助教授 (10218464)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | FISH CELLS / SUSPENSION CULTURE / STATIC-SUSPENSION CULTURE / NON-SERUM CULTURE / EXTRACELLULAR MATRIX / TYPE 1 COLLAGEN / CHSE-ss / CHSE-ns / 浮遊細胞培養 / 高密度培養 / FHM-sp- / CHSE-sp細胞 / 魚コラーゲン / コラーゲン基質 / バイオアッセイ / 細胞培養法 |
|---|
| Research Abstract |
Because of the difficulty that advanced method of fish cell culture such as static-suspension, non-serum, and extracellular matrix-dependent culture method, we examined the characteristics of mainly chinook salmon embryo cells (CHSE) and its derivatives. CHSE-sp cells, which cells completely adapted in suspension-stirred culture and the original of the aim, were selected and developed, and sublines of CHSE-sp were established as CHSE-ss (SS) cells for static-suspension and CHSE-ns (NS) cells for non-serum culture. SS cells stationary incubated at 4ﾟC in hydrophobic surface flask offered single cell suspension without enzymatic digestion and monolayr cell sheet for bio-assay in hydophilic dish even after 1 month. NS cells secreted the cell adhesion and growth substances in the culture medium. Calf type I collagen, inhibited the cell adhesion of CHSE-sp, were permitted the adhesion and spreading of cells by heat denature treatment.
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