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Control of adrenal catecholamine secretion by Ca^<2+> storing mechanisms

Research Project

Project/Area Number 06454121
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

NAKAZATO Yoshikazu  Hokkaido University, Graduate School of Vet. Med., Professor, 大学院獣医学研究科, 教授 (60001525)

Co-Investigator(Kenkyū-buntansha) ITO Shigeo  Hokkaido University, Graduate School of Vet. Med., Associate Professor, 大学院獣医学研究科, 助教授 (40109509)
太田 利男  北海道大学, 獣医学部, 助手 (20176895)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1994: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsATP / Adrenal chromaffin cells / Ca influx / GTP analogues / Catecholamine secretion / Ca stores / Na-Ca exhange / Whole cell voltage clamp / カルシウム電流 / シクロピアゾン酸 / 細胞内カルシウム濃度 / モルモット / カルシウムATPアーゼ
Research Abstract

1. Catecholamine (CA) secretion depending on intracellular Ca store and the effect of extracellular Na : The effect of ryanodine and Ca-ATPase inhibitors on Ca releasing action of caffeine and muscarine from Ca stores was studied in perfused adrenal glands and dispersed chromaffin cells of the quinea pig. In the absence of extracellular Ca, both caffeine and muscarine caused only a small increase in CA secretion which was greatly enhanced after further removal of extracellular Na. Readmission of Ca during perfusion with Na-and Ca-free solution caused inhibition of CA secretion which was reversed to the augmentation by treatment with Ca-ATPase inhibitors.
2. Kinetics of intracellular Ca depending on Ca store and ionic channel activation in single cells : Ryanodine blocked caffeine-induced increase in [Ca]i, but not that induced by muscarine. Inositol 1,4,5, trisphosphate (IP3) and thapsigargin increased[Ca]i and the latter evoked marked inward current. These results suggest that the depletion of intracellular Ca stores in adrenal chromaffin cells may faciliate Ca influx and thus CA secretion through some unknown mechanism as found in smooth muscle cells.
3. Effect of ATP on Ca currents : The effect of ATP on membrane currents was investigated using whole cell voltage clamp techniques. ATP evoked inward current through non-selective cation channels and inhibited Ca currents evoked by depolarizing pulses. The inhibitory effect was abolished by dialysis with GTP analogues. These results suggest that ATP-induced increase in [Ca]i inhibits voltage dependent Ca influx through a mechanism coupled to G protein.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Yoshikazu Nakazato: "Inhibitory effects of caffeine on secretagogue-induced catecholamine secretion from adrenal chromaffin cells of the guinea-pig." British Journal of Pharmacology. 111. 935-941 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Ohata: "Ca^<2+> entry activated by emptying of intracellular Ca^<2+> stores in ileal smooth muscle of the rat." British Journal of Pharmacology. 114. 1165-1170 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ken-ichi Otsuguro: "ATP-evoked membrane current in guinea pig adrenal chromaffin cells." Neuroscience Letters. 187. 145-148 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tadashi Asano: "Muscarinic receptor subtypes mediating catecholamine secretion and increase in intracellular Ca^<2+> concentration in adrenal chromaffin cells of the guinea pig." Journal of Veterinary Medical Science. 57. 1067-1071 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tadashi Asano: "Characteristics of ATP-induced catecholamine secretion from adrenal chromaffin cells of the guinea-pig." Comparative Biochemistry & Physiology-C. 112C. 101-108 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ken-ichi Otsuguro: "Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells." Pflugers Archiv European Journal of Physiology. 429,(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Ohta: "Characterization of cytosolic Ca^<2+> mobilization activated by muscarinic and nicotinic receptors in adrenal chromaffin cells of the guinea pig." Canadian Journal of Physiology and Pharmacology. 72 Suppl. 1. 377 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshikazu Nakazato: "Effects of adenosine 5'-triphosphate (ATP) on catecholamine secretion and membrane currents in adrenal chromaffin cells of the guinea-pig." Canadian Journal of Physiology and Pharmacology. 72 Suppl. 1. 428 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshikazu Nakazato: "Inhibitory effects of caffeine on secretagogue-induced catecholamine secretion from adrenal chromaffin cells of the guinea-pig." British Journal of Pharmacology. 111. 935-941 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Ohta: "All-or-nothing responses to carbachol in single intestinal smooth muscle cells of rat." British Journal of Pharmacology. 112. 972-976 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Ohta: "Ca^<2+> entry activated by emptying of intracellular Ca^<2+> stores in ileal smooth muscle of the rat." British Journal of Pharmacology. 114. 1165-1170 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ken-ichi Otsuguro: "ATP-evoked membrane current in guinea pig adrenal chromaffin cells." Neuroscience Letters. 187. 145-148 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tadashi Asano: "Muscarinic receptor subtypes mediating catecholamine secretion and increase in intracellular Ca^<2+> concentration in adrenal chromaffin cells of the guinea pig." Journal of Veterinary Medical Science. 57 (6). 1067-1071 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tadashi Asano: "Characteristics of ATP-induced catecholamine secretion from adrenal chromaffin cells of the guinea-pig." Comparative Biochemistry & Physiology-C. 112C (2). 101-108 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ken-ichi Otsuguro: "Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells." Pflugers Archiv European Journal of Physiology. 429 (in press.). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Ohta: "Characterization of cytosolic Ca^<2+> mobilization activated by muscarinic and nicotinic receptors in adrenal chromaffin cells of the guinea pig." Canadian Journal of Physiology and Pharmacology. 72 Suppl.1. 377 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshikazu Nakazato: "Effects of adenosine 5′-triphosphate(ATP)on catecholamine secretion and membrane currents in adrenal chromaffin cells of the guinea-pig." Canadian Journal of Physiology and Pharmacology. 72 Suppl.1. 428 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toshio Ohta: "Ca^<2+> entry activated by emptying of intracellular Ca^<2+> stores in ileal smooth muscle of the rat." British Journal of Pharmacology. 114. 1165-1170 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tadashi Asano: "Muscarinic receptor subtypes mediating catecholamine secretion and increase in intracellular Ca^<2+> concentration in adrenal chromaffin cells of the guinea pig." Journal of Veterinary Medical Science. 57. 1067-1071 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tadashi Asano: "Characteristics of ATP-induced catecholamine secretion from adrenal chromaffin cells of the guinea-pig." Comparative Biochemistry & Physiology-C. 112C. 101-108 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ken-ichi Otsuguro: "Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells." Pflugers Archiv European Journal of Physiology. 429(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 中里幸和: "副腎クロム親和性細胞からのホルモン分泌に関与する細胞内Caストアの性質" 秋山記念生命科学振興財団研究成果報告集. 6. 62-65 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toshio Ohta: "Characterization of muscarine-induced Ca^<2+>-transient in chromafin cells of the guinea pig." Japanese Journal of Pharamcology. Suppl.I 64. 193 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshikazu Nakazato: "Inhibitory effects of caffeine on secretagogue-induced catecholamine secretion from adrenal chromaffin cells of the guinea-pig." British Journal of Pharmacology. 111. 935-941 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toshio Ohta: "All-or-nothing responses to carbachol in single intestinal smooth muscle cells of rat." British Journal of Pharamcology. 112. 972-976 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Junji Tsujimatsu: "Effects of Na^+ and Ca^<2+>-ATPase inhibitors on catecholamine secretion evoked by caffeine and muscarine in perfused adrenal glands of the guinea-pig." Japanese Journal of Pharamcology. Suppl.I 67. 160 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ken-ichi Otsuguro: "ATP-evoked membrane current in guineaa pig adrenal chromaffin cells." Neuroscience Letters. 187. 1-4 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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