• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulatory mechanisms of selective growth and death of oocytes

Research Project

Project/Area Number 06454123
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionThe University of Tokyo

Principal Investigator

SATO Eimei  Institute of Medical Science, The University of Tokyo, Associate professor, 医科学研究所, 助教授 (80093243)

Co-Investigator(Kenkyū-buntansha) 豊田 裕  東京大学, 医科学研究所, 教授 (90050418)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1995: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsOocyte maturation / Oogenesis / Apotosis / Cumulus expansion / MAP kinase / Angiogenesis / TNF-alpha / Glycosaminoglycans / 卵成熟 / グリコリミノクリカン / 卵子形成 / 卵胞閉鎖 / 細胞死
Research Abstract

1) The active factor responsible for cumulus expansion was isolated from serum and follicular fluid.
2) DbcAMP was involved in induction of fragmentation of porcine oocytes and HPLC-purified glycosaminoglycans prevented the activation of cAMP-dependent fragmentation.
3) 17alpha-hydroxyprogesterone suppressed the angiogenic activity of hyaluronic acid-like HPLC-purified glycosaminoglycan prepared from extracts of non-luteal mouse ovaries.
4) Immunoreactivity of TNF-alpha was found in the cytoplasm of the cells surrounding the maturing oocytes. Immunoblot analysis with the specific antibody to TNF-alpha identified the 17kD Mr band in the extract of cumulus-oocyte complexes. The process of degeneration of mouse oocytes was modulated by TNF-alpha accumulated in the expanded cumulus during oocyte maturation.
5) Oocyte-cumulus cell complexes influenced the structure of the oviductal wall during the passage in the oviduct.
6) When progesterone and its metabolites are present at high concentrations for a long period, they had the property of inducing in vitro parthenogenetic activation in mouse oocytes.
7) Potential for the development of male half-embryos into fetuses was different from that of female half-embryos.
8) The activation of MAP kinase is involved inthe regulation of meiotic maturation of porcine oocytes, and especially in the regulation after germinal vesicle breakdown.
9) A clone corresponding to mRNA preferentially expressed in the functional staeg of corpus luteum was found to encode a protein similar to the human prostatic secretory protein, which had been originally identified in male genital organs. In contrast, despite the structural similarity in their products, the expression of this newly identified gene was not detected in porcine prostate, suggesting that these structurally related proteins play different roles in mammalian genital organs.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] 佐藤英明: "Structural changes in the oviductal wall during the pasgagc of untertilized cumulus-oocyfe cowplexes in nice" Anatomical Records. 241. 205-210 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤英明: "Seram factor(s) stimalating cumulus expansion in porcine oocyte-cumulus complexes matured and fertilized in vitro" Cell Structure and Function. 20. 223-231 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤英明: "Expressiou of prostatic secretory protein(PSP)-like protein in porcine corpus luteum" Molecular Reproduction and Development. 42. 149-156 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤英明: "17α-Hydroxyprogesterone suppresses neovascularization indured by HPLC-purified ovasian hyaluronicacid like glycosaunoglyian" Endocvine Journal. 42. 675-681 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤英明: "Activation ot nitogen-activated protein kinase during meiotic maturation in porcine oocytes" Zygote. 3. 265-271 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤英明: "新農学シリーズ 家畜繁殖(分担)" 朝倉書店, 173 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤英明: "分子医科学実験プロトゴール(分担)" 秀潤社, 301 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, E., N.Ando, Y.Takahashi, H.Miyamoto and Y.Toyoda: "Structural changes in the oviductal wall during the passage of unfertilized cumulus-oocyte complexes in mice." Anat.Rec.241. 205-210 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Daen, F.P., E.Sato, T.Nakayama, Y.Toyoda: "Serum factor (s) stimulating cumulus expansion in porcine oocyte-cumulus omplexes matured and fertilized in vitro." Cell Struct.Func.20. 223-231 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka, T., K.Itahana, N.Andoh, T.Takeya and E.Sato: "Expression of prostatic secretory protein (PSP)-like protein in porcine corpus luteum : isolation and characterization of a new gene encoding PSP_<94> like protein." Mol.Reprod.Dev.42. 149-156 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okada, M., E.Sato, H.Miyamoto, Y.Toyoda: "17alpha-hydroxyprogesterone suppresses neovascularization induced by HPLC-purified ovarian hyaluronic acid-like glucosaminoglycan in mice." Endocrine J.42. 675-681 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Inoue, M., K.Naito, F.Aoki, Y.Toyoda, E.Sato: "Activation of mitogen-activated protein kinase during meiotic maturation in porcine oocytes Zygote" Zygote. 3. 265-271 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, E.: A new series of agriculture, (Animal Reproduction). Asakura Shoten, (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, E.: Laboratory manual for molecular medical science, (Protocol of embryo manipulation). Shuujyunn-sha, (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤英明: "Structural changes in the oviductal wall during the pasgage of unfertilized cumulus-oocyte complexes in mice" Anatomical Records. 241. 205-210 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤英明: "Serum factor(s) stimulating cumulus expansion in procine oocylecumulus complexes matured and fertilized in vitro" Cell Structure and Function. 20. 223-231 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤英明: "Synchronous sperm penetration of zona-free mouse eggs in vitro" Biology of Reproduction. 53. 424-430 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤英明: "Expression of prostatic secretory protein (PSP)-Oike protein in porcine corpas luteum" Molecular Reproduction and Development. 42. 149-156 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤英明: "172-Hydroxyproqesterone suppresses neovascularization induced by HPLC-parified ovarian hyaluronic acid like qlycosawinoglycau" Endocrine Journol. 42. 675-681 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤英明: "Activation of mitogen-activated protein kinase during meiotic maturation in porcine oocytes" Zygote. 3. 265-271 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤英明: "新農学シリーズ・家畜繁殖(分担)" 朝倉書店, 173 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤英明: "分子医科学実験プロトゴール" 秀潤社, 301 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤英明: "Glycosaminoglycaus prevent induction of fragmentation of porcine oocvfes stimulated by dbcAMP" Cell Stracture and Function. 19. 29-36 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤英明: "The effect of pig follicular fluid fractions on cumulus expausion and male pronacleus formation in porcine oocyfes" Journal of Reproduction and Ferfility. 101. 667-673 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤英明: "Structural changes in the oviductal wall during passage of unfertilized cumulus-oocyte cowplexes in mice" Anafomical Record. 241. 205-210 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤英明: "Morphological evaluation of microvascular networks and angiogenic factors in the selective growth of oocyte and follicles" Acta Anatomica. 149(印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤英明: "卵子の形成と死滅の制御因子" 蛋白質核酸酵素. 39. 62-65 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 豊田 裕: "Association of P34^<cdc2> and cyclin Blduring meiotic maturation in porcine oocytes" Developmental Biology. 165(印刷中). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤英明: "新農学シリーズ・家畜繁殖" 朝倉書店, 173 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤英明: "動物生産学概論" 文永堂(印刷中), (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi