| Project/Area Number |
06454126
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SASAKI Yoshiyuki Kyoto University, School of Agriculture, Professor, 農学部, 教授 (10041013)
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| Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Yukio Kyoto University, School of Agriculture, Instructor, 農学部, 助手 (10252496)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | cattle / fat deposition / adipocyte differentiation / transcription factor / C / EBP / PPAR gamma / obese / major gene / 分子遺伝学 / 脂肪細胞 / 分化 / クローニング / PPAR / ob / 統計遺伝学 |
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| Research Abstract |
Genetic and environmental controls of fat deposition are important for beef production. To study the genetic mechanism of fat deposition, molecular genetics and statistical genetics were adopted.
With the molecular genetics approach, bovine C/EBP alpha, C/EBP beta, C/EBP delta and PPAR gamma2 genes, that encode transcription factors regulating the adipocyte differentiation, were cloned and sequenced. These genes have the high similarities with the mouse genes. The analysis of bovine C/EBP alpha promoter revealed that C/EBP binding and USF binbing sequences, which were important elements of C/EBP alpha gene regulation in mouse and human, were not conserved in cattle, suggesting different mechanism for regulation of bovine C/EBP alpha gene expression. These features in bovine C/EBP alpha promoter were conserved between Japanese Black and Japanese Brown cattles. Moreover, bovine ob gene was cloned.
With the statistical gentetic approach, we investigated how many loci affected the marbling in beef and also whether major gene affected by this trait is present or not. The presence of segregating major gene which has large effect on marbling was expected in Japanese Brown cattle population of Kumamoto Prefecture. Segregating major gene was also detected in some families within that population. Then, this approach is expected as the clue of quantitative trait loci (QTL) analysis with genetic marker.
It is revealed from this study that the joint approach with both molecular and statistical genetics is a very powerful tool to elucidate the genetic mechanism of fat deposition.
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