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Analysis of the prion mechanism using immortalized prionless neuronal cell

Research Project

Project/Area Number 06454128
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionThe University of Tokyo

Principal Investigator

ONODERA Takashi  The University of Tokyo, School of Agriculture and Life Sciences, Department of moleculer immunology, professor, 農学部, 教授 (90012781)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Yasunobu  The University of Tokyo, School of Agriculture and Life Sciences, Department of, 農学部, 助手 (90251420)
MATSUMOTO Yoshitsugu  The University of Tokyo, School of Agriculture and Life Sciences, Department of, 農学部, 助教授 (00173922)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1995: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1994: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordsprion / gene targeting / spongiform encephalopathy / scrapie / neurogenedegenerative diseases / slow virus / kuru / cell immortalization / スクレイビ- / 海綿状脳症 / 痴呆症
Research Abstract

Prion is a cellular protein (PrPc) which has been implicated in transmissible neurodegenerative disease This protein is found on the outer surfaces of neurons of the brain. Although the PrPc function has still not been completely clarified, it seems to be physiologically important because its gene has been found in all mammal examined so far. The establishment of progenitor cell lines from Prn-p gene-target knock out mice embryos and from the wild type would be a valuable model system to clarify if PrPc is related with putative differences in the development of those two cell system and also to verify if there is presence of compensatory gene control mechanisms early in embryogenesis.
Mice homozogous for disrupted Prn-p genes have been generated (Bueler et al. 1987).
From this type of gene-targeted mice, hippocampal cells have been immortalized and clones were obtatined. The hippocampus has been dissected from brain of mouse embryos at embryonic day 14, as the PrPc is predominantly a neuronal protein, presented in a high proportion of hippocampus nerve cell, and that the neurogenesis in vivo are prefferentially occuring at early development.
Twenty eight clones were obtained and has been characterized as prionless and neuronal origin.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 佐伯圭一ら: "Three exon structure of the gene encoding rat prion protein and its expression in tissues." Virus Genes. 12. (in press) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐伯圭一ら: "Identification of a promotor region in the rat prion protein gene." Biochem. Biophs. Res. Comm.120. (in press) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 丹治邦和ら: "Analysis of PrP^C mRNA by in situ hybridization in brain, placenta, uterus and testis of rat." Intervirology. 38. (in press) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小野寺節: "プリオン" 生化学. 67. 1249 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小野寺節: "牛海綿状脳症とプリオン説" モダンメディア. 40. 60-68 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Keiichi Saeki et al.: "Three exon structure of the gene encoding rat prion protein and its expression in tissues." Virus Genes.12 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Keiichi Saeki et al.: "Identification of a promotor region in the rat prion protein gene." Biochem.Biophs.Res.Comm.120 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kunikazu Tanji et al.: "Analysis of PrP^C mRNA by in situ hybridization in brain, placenta, uterus and testis of rat." Intervirology. 38 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Onodera: "Prion (in Japanese)" Biochemistry (in Japanese). 67. 1249 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Onodera: "Bovine spongiform encephalopathy and prion hypothesis. (in Japanese)" Modern media (in Japanese). 40. 60-68 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐伯圭一ら: "Three exon structure of the gene encoding rat prion protein and its expression in tissues." Virus Genes. 12(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐伯圭一ら: "Identification of a promotor region in the rat prion protein gene." Biochem.Biophs.Res.Comm.120(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 丹治邦和ら: "Analysis of PrP^C mRNA by in situ hybridization in brain,placenta,uterus and testis of rat." Intervirology. 38(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小野寺節: "プリオン" 生化学. 67. 1249 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小野寺節: "牛海綿状脳症とプリオン説" モダンメディア. 40. 60-68 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Onodera T.,他: "Diversity of clinical sign in natural scrapie cases in Japan." Jpn. Agri. Res. Quart.28. 59-61 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐伯 圭一,他: "ラット不死化神経細胞株およびラットにおけるプリオン蛋白のcDNAのクローニングと遺伝子発現の解析" 家畜生化学. 31. 37-43 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小野寺 節: "牛海綿状脳症のその後について" モダンメディア. 41. 60-68 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小野寺 節: "近代出版(山内一也、立石潤、編)" スローウイルス感染とプリオン, 300(25) (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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