| Project/Area Number |
06454131
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | MIYAZAKI UNIVERSITY |
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Principal Investigator |
OGAWA Hiroyuki MIYAZAKI UNIV.VET.SURG.ASSOC.PROF., 農学部, 助教授 (30012016)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Ryozi MIYAZAKI UNIV.VET.PATHOL.ASSOC.PROF., 農学部, 助教授 (90150169)
GOTO Yositaka MIYAZAKI UNIV.VET.MICROBIOL.ASSOC.PROF., 農学部, 助教授 (30142136)
KASEDA Yuziro MIYAZAKI UNIV.VET.HOSPITAL PROF., 農学部, 教授 (70041019)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1996: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Bovine / Bleeding disorder / Platelet dysfunction / von Willebran factor / Factor XIII / Cenetic screening / von Willebrand因子 / 血小板 / von Willbrand 因子 / 遺伝子スクリーニング |
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| Research Abstract |
From our investigations on three hundred of bleeding Japanese black cattle since 1982, we have reported that the sporadic hemorrhagic disease is not a single disease, but it originates in three diseases of Chediak-Higashi syndroml, hemophilia like disease and factor XIII deficiency. In this study, we attempt to find the carrier detection method in these bleeding disorders.
1) Segregation analyzes and quntitative genetic analyzes showed that Chediak-Higashi syndrome (C-HS), hemophilia like disease and factor XIII deficiency in cattle were simple autosomal recessive traits.
2) Von Willebrand factor activity assay and multimer analysis showed that Hemophilia like disease was von Wliiebrand disease type III.
3) Screening method for carrier detectin was not established in C-HS and vWd.
4) In factor XIII deficiency, plasma factor XIII activity was decreased in carriers and A mutation in the gene for factor XIII subunit A was detected and linkage of this mutation with the defect was confirmed. Then, factor XIII carriers could be screeened out by PCR-RFLP method successfully. Ten percent of sires tested were found to be carriers.
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