| Project/Area Number |
06454138
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
IKEGAMI Susumu Hiroshima University, Dept.Applied Biochemistry, Professor, 生物生産学部, 教授 (80011980)
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| Co-Investigator(Kenkyū-buntansha) |
SHIODA Masaki Kumamoto University, Dept Biological Sciences, Professor, 理学部, 教授 (80134526)
HOSOYA Hiroshi Hiroshima University, Dept.Biological Sciences, Professor, 理学部, 教授 (90183102)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | Myosin / Phosphorylation / P1 / NAAP / cdc2 kinase / Cyclin B / ヒトデ / 細胞核 / 二重鎖RNA / 卵細胞 / cDNA / 卵母細胞 / ハレナキノール・サルフェート / ジャスピシン / イリジャスピシン / DNAポリメラーゼ / 細胞膜融合 / 孵化酵素 / ゲラチナーゼA / 蛋白質セリン・キナーゼ |
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| Research Abstract |
Hosoya and his colleagues discovered that phosphorylated myosin is present in the contractile ring. This is a very importand discovery for the understanding of the mechanism of cell division. They also discovered the enzyme that selectively phosphorylates the light chain of myosin.
Shioda and his colleagues discovered a novel protein, P1, which regulates the initiation of DNA replication. P1, which is inactive in its unphosphorylated form, becomes active by phosphorylation. The enzyme responsible for the phosphorylation is found to be a DNA-dependent kinase.
Ikegami and his colleagues discovered a novel nucleic-acid associated protein designated as NAAP which was isolated from starfish oocytes. Oligonucleotides derived from the partial amino acid sequences of NAAP were used to clone NAAPcDNA and the entire cDNA sequence was determined. The transcript encoding NAAP was present only in growing oocytes but not in full-grown oocytes, embryos and adult tissues. The NAAP protein localized in the germinal vesicle of the full-grown oocyte and dispersed diffusely after germinal vesicle breakdown at the onset of meiosis. During oocyte maturation, NAAP changed the moleclar form in a cell-cycle dependent manner. It was found that Ser 145 of NAAP,which was unphosphorylated at the germinal vesicle stage, was phosphorylated during the M phase of meiosis.
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