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Studies on Plasmalemmal Caveola

Research Project

Project/Area Number 06454141
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionGunma University (1995)
Kyoto University (1994)

Principal Investigator

FUJIMOTO Toyoshi  School of Medicine, Gunma University, 医学部, 教授 (50115929)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1995: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1994: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsCaveola / Plasma membrane / Calcium / Actin / Inositol Trisphosphate / GPI-anchored protein / Glycolipid / Cytoskeleton / スフィンゴミエリン
Research Abstract

1) Plasmalemmal inositol trisphosphate receptor-like protein (PM-IP3R) is localized in caveolae. We investigated its role in the linkage of caveolae and actin filaments. In normal Pam 212 cells, PM-IP3R was distributed densely in the vicinity of intercellular contacts where actin filaments were also cencentrated. In cytochalasin D-treated cells, PM-IP3R was localized in spots where disorganized actin was aggregated ; by electron microscopy, the labeling for PM-IP3R was seen around caveolae appearing like a bunch of grapes. PM-IP3R was not solubilized by Triton X-100 or octylglucoside. These results indicate that PM-IP3R may mediate the linkage of caveolae and actin filaments.
2)Distribution of GPI-anchored proteins, glycolipids and sphingomyelin was examined by immunocytochemical techniques. When cultured cells were treated with antibodies to the molecules, and then incubated with labeled secondary antibodies, the labeling was distributed in the entire surface. But when the cells were warmed to 37C after treated with the primary and secondary antibodies, the labeling was concentrated to caveolae after 10 to 30 min. During the process, GPI-anchored proteins and glycolipids, or GPI-anchored proteins and sphingomyelin, formed common patches and then accumulated to common caveolae. The redistribution was observed even in the presence of cytochalasin D and/or nocodazole. The results indicate that sequestration of GPI-anchored proteins, glycolipids and sphingomyelin occurs only when the molecules are cross-linked by antibodies, and does not depend on intact cytoskeleton.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Fujimoto, T., A. Migawaki, and K. Mikoshiba: "Inasitol 1. 4. 5-trisphosphate recepter-like protein in plasmalemmal caveolal is linked to actin filaments" J. Cell Sci.108. 7-15 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Aoki, T., K. Taira S. Shibasaki and T. Fujimoto: "The cytochemical and immunohistochemical study of septal cells in the rat lung" Acta Histochem. cytochem.28. 349-355 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Amino,K., Takahashi, M., Y. Honda and T. Fujimoto: "Redistribution of fodrin in an in Vitro wound healing modeal of the corneal epithelium." Exp. Eye Res.61. 501-508 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 藤本豊士: "日本組織細胞化学会編「組織細胞化学1994」" 学際企画, 7 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujimoto, T., A.Miyawaki, and K.Mikoshiba: "Inositol 1,4,5-trisphosphate receptor-like protein in plasmalemmal caveolae is linked to actin filaments" J.Cell Sci.108. 7-15 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Aoki, K., K.Taira, S.Shibasaki and T.Fujimoto: "The cytochemical and immunohistochemical study of septal cells in the rat lung." Acta Histochem.Cytochem.28. 349-355 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Amino, K., Takahashi, M., Y.Honda and T.Fujimoto: "Redistribution of fodrin in an in vitro wound healing model of the corneal epithelium." Exp.Eye Res. 61. 501-508 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fukui, M., R.Yoshida, K.Watanabe, T.Fujimoto, M.Ohi and K.Kuno: "Hypoxic contraction of alveolar interstitial cells isolated from bovine lung." Am.J.Physiol.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Amino, K., Takahashi, M., Y.Honda and T.Fujimoto: "Tyrosine phosphorylation in desmosomes and hemidesmosomes of the corneal epithelium." J.Histochem.Cytochem.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fukui, M., T.Fujimoto, K.Watanabe, and K.Kuno: "Prostaglandin F synthase is localized to contractile interstitial cell in the boving lung." J.Histochem.Cytochem.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujimoto, T.: "GPI-anchored proteins, glycosphingolipids, and sphingomyelin are sequestered to caveolae only after cross-linking." (Submitted for publication.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujimoto, T., Miyawaki and K. Mikoshiba: "Inositol 1,4,5-trisphosphate receptor-like protein in plasmalemmal coveclal is linked to actin filaments." J. cell Sci.108. 7-15 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Aoki, T., K. Taira, S. Shibasaki and F. Fujimoto: "The cytochemical and immunahisto chemical study of septal cell in the rat lung" Acia Histochem. Cytochem.28. 349-355 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Amino, K., Takahashi, M., Y. Hondo and T.Fujimoto: "Redistribution of fodrin in an in vitro wound healing modeal of the corneal eptithelium" Exp. Eye. Res.61. 501-508 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 藤本豊士: "日本組織細胞化学会編「組織細胞化学1994」" 学際企画, 7 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toyoshi Fujimoto: "Inositol 1,4,5-trisphosphate recepter-like protein in plasmalemual caveclae is liked to actin filaments" Journal of Cell Science. 108. 7-15 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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