• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Southwestern histochemical localization of regulatory element binding proteins for rat anterior pituitary hormones.

Research Project

Project/Area Number 06454143
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNagasaki University

Principal Investigator

NAKANE Paul K.  Nagasaki University, School of Medicine, Department of Histology and Cell Biology. Professor and Chairperson, 医学部, 教授 (60164240)

Co-Investigator(Kenkyū-buntansha) ABE Kuniko  Nagasaki University, School of Medicine, Department of Histology and Cell Biolog, 医学部, 助手 (00253641)
SHIN Masashi  Nagasaki University, School of Medicine, Department of Histology and Cell Biolog, 医学部, 助手 (80145226)
IZUMI Shin-ichi  Nagasaki University, School of Medicine, Department of Histology and Cell Biolog, 医学部, 助手 (40264246)
KOJI Takehiko  Nagasaki University, School of Medicine, Department of Histology and Cell Biolog, 医学部, 助教授 (30170179)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1994: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywordsregulatory elements / regulatory element binding protein / growth hormone / prolactin / anterior pituitary / GH3 cells / Southwestern histochemistry / Immunohistochemistry / South-Western Histochemistry / 下垂体細胞
Research Abstract

For the activation or suppression of a gene, the formation of stable molecule complexes between regulatory elements (RE) of the gene and binding proteins for the regulatory elements is often a prerequisite (REBP). Pit-1/GHF-1 form complex with RE of growth hormone (GH) (GH-1) and activates rat GH gene and it also binds to RE of prolactin (PRL) (P1) and activates PRL gene. At the same time, the binding of GH gene promoter proximal repressor element binding protein (PREB) to GH gene promoter proximal repressor element (PRE) suppress GH gene. In GH3 cells in vitro and in sections of rat anterior pituitary, Pit-1/GHF-1 and PREB were localized by utilizing a newly introduced Southwestern histochemical method (SWH). Pit-1/GHF-1 protein, GH and PRL were localized by immunohistochemistry (IHC). Some tissue sections were used for the double staining by SWH and IHC.For the localization of REBP,oligodeoxynucleotides (oligo-DNAs) correspond to rat GH-1 (-89 and -70) and rat PRE (-169 and -152) were used. SWH revealed that Pit-1/GHF-1 was mainly found in nuclei of GH or PRL cells as well as in nuclei of some other cells except gonadotrophs, whereas nuclear localization of the PREB was mainly in PRL cells and some other cells except GH cells and gonadotrophs in the rat pituitary tissue sections. Cell nuclei positive for Pit-1/GHF-1 by SWH were also positive for Pit-1/GHF-1 proteins by IHC.In GH3 cells in vitro, the results similar to that seen in sections of anterior pituitary were found.
Our findings support a notion that the formation of complex between GH-1 and Pit-1/GHF-1 is needed for the activation of GH gene and that the formation of complex between P1 and Pit-1/GHF-1 is needed for the activation of PRL gene. If appears that the formation of complex between PRE and PREB suppressed GH gene in PRL producing cells. A mechanism that suppresses PRL gene in GH producing cells remains to be investigated.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report

Research Products

(12 results)

All Other

All Publications

  • [Publications] Koji,T: "Localization of cyclic adenosine 3,5 -monophosphate-responsive element (CRE)-binding proteins by southwestern histochemistry." J Histochem Cytochem. 42. 1399-1405 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Koji,T: "Recent advances in molecular histochemical techniques : In situ hybridization and southwestern histochemistry." J Electron Microsc. 45. 119-127 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Izumi,S: "Localization of transcription factors on the growth hormone gene promoter in rat anterior pituitary cells by the southwestern histochemistry." Acta Histochem Cytochem.29. 261-26 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 小無田 興: "組織細胞化学 1995" 学際企画, 198 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Koji, T.: "Localization of cyclic adenosine 3', 5'-monophosphate-responsive element (CRE)-binding proteins by Southwestern histochemistry." J Histochem Cytochem. 42. 1399-1405 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Koji, T.: "Recent advances in molecular histochemical techniques : In situ hybridization and Southwestern histochemistry." J Electron Microsc. 45. 119-127 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Izumi, S.: "Localization of transcription factors on the growth hormone gene promoter in rat anterior pituitary cells by the southwestern histochemistry." Acta Histochem Cytochem. 29. 261-262 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Komuta, K.: "Southwestern histochemistry." Gakusai-Kikaku (Tokyo), Histochemistry and Cytochemistry. 1995. 198 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Koji,T: "Recent advances in molecular histochemical techniques : In situ hybridization and southwestern histochemistry." J Electron Microsc.45・2. 119-127 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Izumi.S: "Localization of transcription factors on the growth hormone gene promoter in rat anterior pituitary cells by the southwestern histochemistry." Acta Histochem. Cytochem.29・Supplemest. 261-262 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 小無田 興: "組織細胞化学1995 日本組織細胞化学会編" 学際企画, 198 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] TAKEHIKO KOJI.KOMUTA MASAYUKI NOZAWA.SHUHEI YAMADA and PAUL K.NAKANE.: "Localization of Cyclic Adenosine 3',5'-Monophosphate-responsive Element(CRE)-binding Proteins by Sounthwestern Histochemistry." The journal of Histochemistry and Cytochemistry. 42. 1399-1405 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi