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Regulation of exocytotic release of neurotransmitters by Ca^<2+> and associated proteins.

Research Project

Project/Area Number 06454146
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionTohoku University (1995)
Kyoto University (1994)

Principal Investigator

YAWO Hiromu  Tohoku University, School of Medicine, Professor, 医学部, 教授 (00144353)

Co-Investigator(Kenkyū-buntansha) 遠藤 克昭  京都大学, 医学部, 助手 (30025613)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1995: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1994: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywordssynapse / presynaptic / exocytosis / calcium / adrenergic / noradrenaline / neuromodulator / ciliary ganglion / 細胞内カルシウム / 蛍光カルシウム指示薬 / fura-2 / 開口放出 / 自律神経 / シナプス小胞
Research Abstract

The giant presynaptic terminal of chick ciliary ganglion was used to examine how noradrenaline (NA) modulates neurotransmitter release. The cholinergic excitatory synaptic currents (EPSCs) were recorded under whole-cell voltage clamp of the postsynaptic neuron. Although the EPSC was potentiated by NA,the current directly activated by acetylcholine (I_<ACh>) was unaffected. NA also increased the quantal contents without changing the quantal size. The NA-dependent potentiation was antagonized by neither phentolamine nor propranolol. The EPSC was also potentiated by adrenaline and dopamine but not by normetanephrine, phenylephrine or isoproterenol. The EPSC was attenuated by clonidine. Therefore, NA potentiated the transmitter release through a receptor pharmacologically different from both alpha- or beta- adrenergic receptors. The Ca^<2+> increment produced by an action potential (DELTA[Ca^<2+>]_<pre>) was reduced by NA through an alpha_2-adrenergic receptor. However, when alpha_<2+>-adrenergic receptors were blocked, neither DELTA[Ca^<2+>]_<pre> nor resting Ca^<2+> were changed by NA.The [Ca^<2+>]_o-EPSC relation was shifted by NA, decreasing the half saturating [Ca^<2+>]_o, without changing the maximum. It is concluded that NA-dependent potentiation of transmitter release was due to an increase in the Ca^<2+> sensitivity of the exocytotic process. The enhancement of the fusion probability is suggested.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Yawo, H. et al.: "Modulation of presynaptic calcium channels by neurotransmitters." Biocned. Res.15. 9-16 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Endo, K. and Yawo,H.: "Cellulan mechanisms of enkephalin action on transmitter release from the giant presynaptic terminal of chick ciliary ganglion." Jpn. J. Physiol.44. S177- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yawo, H. and Endo, K.: "Facilitation of Transmitter ralease by noradrenaline from the giant presyraptic terminal of chick ciliary garglion." Neurosci. Res. Suppl.19. S30- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yawo, H.: "Noradrenaline modulates transmitter release by enhancing the Ca^<2+> sensitivity of exocytosis in the chick ciliary Guglion" J. Physiol. Lond.(印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yawo, H.et al.: "Modulation of presynaptic calcium channels by neurotransmitters." Biomed.Res.15, suppl. 1. 9-16 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Endo, K.and Yawo, H.: "Cellular mechanisms of enkephalin action on transmitter release from the giant presynaptic terminal of the chick ciliary ganglion." Jpn J.Physiol.44, suppl. 1. S177 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yawo, H.and Endo, K.: "Facilitation of transmitter release by noradrenaline from the giant presynaptic terminal of chick ciliary ganglion." Neurosci. Res.Suppl. 19. S30 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yawo, H.: "Noradrenaline modulates transmitter release by enhancing the Ca2+sensitivity of exocytosis in the chick ciliary presynaptic terminal." J.Physiol. Lond.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yawo,H.et al.: "Modulation of presynapfic calcium channels by neurotransmitters." Biomed,Res. 15. 9-16 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Endo,K.and Yawo,H.: "Cellular mechanisms of enkephalin action on transmitter release from the giant presynapfic terminal of chick ciliary gonglion." Jpn.J.Physiol.44. S177 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yawo,H.and Endo,K.: "Facilitation of transmitter release by noradre naline from the giant presynapfic terminal of chick ciliary gonglion" Neurosci.Res.Suppl.19. S30 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yawo,H.: "Noradrenaline modulates transmitter release by Cnhancing the Ca^<2+> sensitivity of eaocytosis in the chick ciliary presynapfic terminal" J.Physiol.Lond.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 八尾 寛: "ホールセル記録法と穿孔パッチ法" 日本生理誌. 56. 305-331 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 八尾 寛: "Modulafion of pie symyohic calciun channels by neurotransniHers" Biomed.Res.15 suppl 1. 9-16 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 八尾 寛: "ω-Conofoxin-sensifive and-resisfant fronsmiHer relossl from the chick ciliary presynabhic ferminal." J.Dlysiol.(Lond.). 477. 437-448 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 八尾 寛: "アデノシンの伝達物質放出抑制機序" 実験医学. 12. 61-63 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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