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TEMPERATURE RECEPTIVITY IN DISSOCIATED HYPOTHALAMIC NEURONS AND CULTURED PC-12 CELLS

Research Project

Project/Area Number 06454152
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionKYUTO INSTITUTE OF TECHNOLOGY

Principal Investigator

KIYOHARA Toshikazu  Kyoto institute of Technology Department of Applied Biology, Professor, 繊維学部, 教授 (50071874)

Co-Investigator(Kenkyū-buntansha) MIYATA Seiji  Kyoto Institute of Technology Department of Applied Biology, Research Associate, 繊維学部, 助手 (30243124)
NAKASHIMA Toshihiro  Kyoto Institute of Technology Department of Applied Biology, Associate Professor, 繊維学部, 助教授 (30128136)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1995: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsTemperature receptivity / Whole cell clamp / Cold sensitivity / c-fos / Cold acclimation / カルシウム動態 / パッチクランプ法
Research Abstract

1, Rat pheochromocytoma cells (PC-12) were treated with nerve growth factor for 5 days. The effects of cooling on membrane characteristics and ionic currents were investigated. All cells tested showed depolarization associated with increase in input membrane resistance by cooling. Cooling also increased voltage-dependent outward-rectifier and sodium inward currents. The results suggest that PC-12 cells have some characteristics of cold-sensitive neurons and have sodium and potassium channels which respond non-linearly to temperature change.
2, Changes in the immunoreactivity for c-fos in the rat brains were investigated during cold acclimation. The sites of c-fos immunoresponded cells were classified into three types on the basis of time course of the c-fos expression. First, c-fos immunoreactivity was seen in the animals exposed to the cool environment for 3 h and 24 h, but not found in the cold adapted (14 days). Second, c-fos positive cells were persistently found in the rats exposed to cold from 3 h to 14 days. Third, c-fos immunoreactivity were not observed in the animals exposed to the cooling for 3 h and 24 h, but it appeared in the cold adapted. These data reveal that changes in the sites of c-fos immunoreactive cells are correlated with the alternation of thermogenic mechanism in the rat brains with cold acclimation.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] T. Nakashima: "Naloxone suppresses the rising phose of fever induced by interferon-d." Brain Research Bulletin. 37. 61-66 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S. Miyata: "Tempord changes of c-fos expression in oxytocinergic magnocellular neuroendcrine cells of the rat hypothalamus with restraint stress." Brain Research Bulletin. 37. 391-395 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S. Miyata: "Maintenance of ultrastructural plasticity of the hypothalamic supraoptic nucleus in the ovariectomized rat." Brain Research Bulletiv. 37. 405-409 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S. Miyata: "Central mechanism of neural activation with cold acclimation of rat using Fos immunohistochemistry" Meuroscience Research. 22. 209-218 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S. H. Lin: "Fos expression in the hypothalamic magnocellullar neurons of rats during pregnacy,parturition and lactation." Neuroscience Research. 23. 29-34 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Kiyohara: "Differences in Fos expression in the rat brains between cold and warm amblient exposures" Brain Research Bulletin. 38. 193-201 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Nakashima: "Body Temperature and Matabolism" eds. T. Nagasaka & A. S. Milton Inrernational Press Editing Center Incorporation (Tokyo), 9-12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S. Miyata: "Body Temperature and Matabolism" eds. T. Nagasaka & A. S. Milton Inrernational Press Editing Center Incorporation (Tokyo), 13-16 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 1. S.Miyata, T.Itoh, O.Matsushima, T.Nakashim & T.Kiyohara: "Not only osmotic stress but also repeated restraint stress causes structural plasticity in the supraoptic nucleus of the rat hypothalamus." Brain Res.Bull.33. 669-675 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 2. S.Miyata, T.Nakashima & T.Kiyohara: "Structural dynamics of neural plasticity in the supraoptic nucleus of the rat hypothalamus during dehydration and rehydration." Brain Res.Bull.34. 169-175 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 3. S.Miyata, T.Nakashima & T.Kiyohara: "Expression of c-fos immunoreactivity in the hypothalamic magnocellular neuron during chronic somatic stimulation." Neurosci.Lett.175. 63-66 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 4. T.Nakashima, T.Murakami, Y.Murai, T.Hori, S.Miyata & T.Kiyohara: "Naloxone suppresses the rising phase of fever induced by interferon-alpha." Brain Res.Bull.37. 61-66 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 5. S.Miyata, T.Itoh, S-H.Lin, M.Ishiyama, Y.Nakashima & T.Kiyohara: "Temporal changes of c-fos expression in oxytocinergic magnocellular neuroendocrine cells of the rat hypothalamus with restraint stress." Brain Res.Bull.37. 391-395 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 6. S.Miyata, S.-H.Lin, T.Kawarabayashi, T.Nakashima & T.Kiyohara: "Maintenance of ultrastructural plasticity of the hypothalamic supraoptic nucleus in the ovariectomized rat." Brain Res.Bull.37. 405-409 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 7. S.Miyata, M.Ishiyama, O.Shido, T.Nakashima, M.Shibata & T.Kiyohara: "Central mechanism of neural activation with cold acclimation of rats using Fos immunohistochemistry." Neurosci.Lett.22. 209-218 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 8. S.-H.Lin, S.Miyata, T.Itoh, T.Kawarabayashi, T.Nakashima & T.Kiyohara: "Fos expression in the hypothalamic magnocellular neurons of rats during pregnancy, parturition and lactation." Beurosci.Res.33. 28-34 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 9. T.Kiyohara, S.Miyata, T.Nakamura, O.Shido, T.Nakashima & M.Shibata: "Differences in Fos expression in the rat brains between cold and warm ambient exposures." Brain Res.Bull.38. 193-201 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Nakashima: "Naloxone suppresses the rising phase of fever induced by interferon-d" Brain Research Bulletin. 37. 61-66 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Miyata: "Temporal changes of c-fos expression in oxytocinergic magnocellular neurcendocrine cells of the rat hypochalamus with cestraint stress." Brain Research Bulletin. 37. 391-395 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Miyata: "Maintenance of ultrastructural plasticity of the hypothalamic supraoptic nucleus in the ovariecromized rat." Brain Research Bulletin. 37. 405-409 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Miyata: "Central mechanism of neural activation with Cold acclimation of rat using Fos immuno histochemistry" Neuroscience Research. 22. 209-218 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.-H.Lin: "Fos expression in the hypothalamic magnocellular neurons of rats dcering pregnancy, parturition and lactation" Neuroscience Research. 23. 29-34 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Kiyohara: "Differences in Fos expression in the rat brains between cold and warm ambient exposures" Brain Research Bulletin. 38. 193-201 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Nakashima: "Body Temperature and Metabolism" eds. T. Nagasaka & A. S. Milton lnternational Press Editing Center Incorporation (Tokyo), 9-12 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Miyata: "Body Temperature and Metabolism" eds. T. Nagasaka & A. S. Milton lnternational Press Editing Center Incorporation (Tokyo), 13-16 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Miyata: "Not only osmotic stress but alsc repeated restraint stress causes structural plasticity in the supraoptic nucleus of the rat hypothelamus." Brain Research Bulletin. 34. 669-675 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Miyata: "Expression of c-fosimmunoreactivity in the hypothalamic magnicellular neuron during chronic osinotic stimulation" Neuroscience Letters. 175. 63-66 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Miyata: "Structural dynamics of neural plasticity in the supraoptic nucleus of the rat hypothalamus during dehydration and rehydration" Brain Research Bulletin. 34. 169-175 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Nakashima: "Naloxone suppresses the rising phase of fever induces by interferon-α" Brain Research Bulletin. 36(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Miyata: "Temporal changes of c-fos expression in oxytocinergic magnocellular neuroendocrine cells of the rat hyposhalamus with restraint srress" Brain Reseach Bulletin. 37(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Miyata: "Central mechamisms of neural activation with cold accli mation of rats using fos immunohisso chemistry" Neuroscience Research. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 清原壽一: "脳機能の解明-分子から病態まで-" 赤池紀扶・小暮久也編(創風社), 123-128 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Nakashima: "Integrtive and cellutar aspects of autonomic functionsi temperature and osmoregu lation" eds,K,Pleschka & R.Gersbersger John Libbey Eurotext(Paris), 417-475 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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