| Project/Area Number |
06454199
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | TOTTORI UNIVERSITY |
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Principal Investigator |
HIRAI Kazumitsu Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (20093940)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUMOTO Soji Tottori University, Faculty of Medicine, Asociate Professor, 医学部, 助教授 (60111126)
SATO Kenzou Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (40113196)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | Spirometra erinaceieuropaei / Plerocercoid / Growth factor / Cathepsin-L like substance / Growth hormone / Host-parasite relationship / 擬充尾虫 / 宿主-寄生体関係 / システインプロテイナーゼ |
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| Research Abstract |
We conducted various experiments to clarify properties of the growth hormone (GH)-like substance produced by plerocercoids of Spirometra erinaceieuropaei, and got results as follows :
1. The worm extract creavaged the anti-27kDa protein antibody as antibody against GH-like substance, when the worm extract was incubated with the biotinylated anti-27kDa protein antibody.
2. This ability of the extract was inhibited by addition of cystein proteinase inhibitors such as E64 and leupeptin.
3. The ^<125>I-anti-27kDa proten antibody was incorporated into plerocercoid bodidy when plerocercoids were cultured with ^<125>I-anti-27kDa protein antibody and incorporation of antibody was enhanced with increase in antibody added and duration of culture.
4. Murine macrophages activated by the excretory/secretory (ES) products of plerocercoids expressed the mRNAs of TNF-alpha and IL-6
5. Macrophages cultured with ES products were suppressed expression of nitric oxide synthase, TNF-alpha and IL-6 mRNA in LPS-activated macrophages but enhanced expression of IL-6 mRNA in IFN-gamma activated macrophages.
These results suggest that 27 kDa protein and other products produced by plerocercoids inhibit humoral and cellural immunity of the host.
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