| Project/Area Number |
06454203
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Chiba University |
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Principal Investigator |
NODA Masatoshi Chiba University, School of Medicine, Chairman and Professor, 医学部, 教授 (60164703)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAKE Masami Chiba University, School of Medicine, Research Assistant, 医学部, 助手 (10251175)
盛永 直子 千葉大学, 医学部, 講師 (20092108)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | cytotoxin / MRSA / Vero cells / CHO cells / RSAaUV^r cells / lethal activity |
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| Research Abstract |
Two novel cytotoxins designated HL-cytotoxin and HS-cytotoxin of methicillin-resistant Staphylococcus aureus (MRSA) which was isolated from a patient hospitalized at an intensive care unit were purified. These purified toxins were distinctly different from staphlococcal exotoxins reported already such as toxic shock syndrome toxin 1 (TSST-1), enterotoxins, alpha-toxins, delta-toxins, leukocidin and exofoliative toxins. The molecular weights of the HL-cytotoxin and HS-cytotoxin were approximately 50,000 and 40,000, respectively. These toxins were cytotoxic to Vero cells, CHO cells and RSa-UV^r cells which are human embryonic cells transformed Rous sarcoma virus. The HL-cytotoxin lost its cytotoxic activity when it was heated at 60 C for 10 min. On the other hand, the cytotoxicity of the HS-cytotoxin was still stable when it was heated at 80 C for 30 min. No hemolytic activity of these toxins to human and rabbit erythrocytes was observed. The HL-cytotoxin and the HS-cytotoxin possessed also lethal activities to mice. Further experiments attempting to clarify the mode of action of these toxins purified from MRSA on RSa-UV^r cells are in progress.
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