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Novel cytotoxins purified from methicillin-resistant staphylococcus aureus

Research Project

Project/Area Number 06454203
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Bacteriology (including Mycology)
Research InstitutionChiba University

Principal Investigator

NODA Masatoshi  Chiba University, School of Medicine, Chairman and Professor, 医学部, 教授 (60164703)

Co-Investigator(Kenkyū-buntansha) MIYAKE Masami  Chiba University, School of Medicine, Research Assistant, 医学部, 助手 (10251175)
盛永 直子  千葉大学, 医学部, 講師 (20092108)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥5,400,000 (Direct Cost: ¥5,400,000)
Keywordscytotoxin / MRSA / Vero cells / CHO cells / RSAaUV^r cells / lethal activity
Research Abstract

Two novel cytotoxins designated HL-cytotoxin and HS-cytotoxin of methicillin-resistant Staphylococcus aureus (MRSA) which was isolated from a patient hospitalized at an intensive care unit were purified. These purified toxins were distinctly different from staphlococcal exotoxins reported already such as toxic shock syndrome toxin 1 (TSST-1), enterotoxins, alpha-toxins, delta-toxins, leukocidin and exofoliative toxins. The molecular weights of the HL-cytotoxin and HS-cytotoxin were approximately 50,000 and 40,000, respectively. These toxins were cytotoxic to Vero cells, CHO cells and RSa-UV^r cells which are human embryonic cells transformed Rous sarcoma virus. The HL-cytotoxin lost its cytotoxic activity when it was heated at 60 C for 10 min. On the other hand, the cytotoxicity of the HS-cytotoxin was still stable when it was heated at 80 C for 30 min. No hemolytic activity of these toxins to human and rabbit erythrocytes was observed. The HL-cytotoxin and the HS-cytotoxin possessed also lethal activities to mice. Further experiments attempting to clarify the mode of action of these toxins purified from MRSA on RSa-UV^r cells are in progress.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] 野田公俊: "MRSAの毒素産生" 臨床医. 21. 362-366 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野村 文夫: "ラット肝細胞膜蛋白のADP-リボシル化反応に及ぼす長期飲酒の影響" アルコールと医学生物学. 15. 119-122 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tetsuo Nishikawa, et al.: "Cholera toxin can ADP-ribosylate Gs as Gi in ACTH unresposive humanadrenocortical cancer." Endocrine Journal. 41. 593-597 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tetsuo Nishikawa, et al.: "Possible involement of lipxygenase metabolites of arachidonic acid in the regulation of pregnenolone synthesis in bovine adrenocortical mitchondria." J. Biochem.116. 833-837 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Naoko Morinaga, et al.: "Differentiation of HL-60 cells is prometed by H-toxin of Clostridium septicum." FEBS Lett.351. 317-320 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuo Nagase, et al.: "Primiting effect of hydroxyapatite on the chemiluminescence response in human polymorphonuclear leukocytes." FEBS Lett.349. 13-16 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masatoshi Noda, Joel Moss, et al: "Bacterial toxins and virulence factors in disease" Marcel Dekker, Inc, New York, 627 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野田 公俊 竹田美文: "コレラ菌感染症の免疫学" 国際医書出版,東京, 504 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tetsuo Nishikawa et al.: "Cholera toxin can ADP-ribosylate GS as Gi in ACTH unresposive humandrenocortical cancer." Endocrine Journal. 41. 593-597 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tetsuo Nishikawa et al.: "Possible involement of lipxygenase metabolites of arachidonic acid in the regulation of pregnenolone synthesis bovine adrenocortical mitchondria." J.Biochem.116. 833-837 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Naoko Morinaga et al.: "Differentiation of HL-60 cells is prometed by H-toxin of clostridium septicum." FEBS Lett.351. 317-320 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuo Nagase et al: "Priming effect of hydroxyapatite on the chemiluminescence response in human polymorphonuclear leukocyts." FEBS Lett.349. 13-16 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masatoshi Noda, Joel Moss et al.: Bacterial toxins and virulence factors in disease.Marcel Dekker, Inc., New York, 627 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野田公俊: "MRSAの毒素産生" 臨床医. 21. 362-366 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 野村文夫: "ラット肝細胞膜蛋白のADP-リボシル化反応に及ぼす長期飲酒の影響" アルコールと医学生物学. 15. 119-122 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tetsuo Nishikawa et al.: "Cholera toxin can ADP-ribosylate Gs as Gi in ACTH unresposive humanadrenocortical cancer." Endocrine Journal. 41. 593-597 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tetsuo Nishikawa et al.: "Possible involement of lipxygenase metabolites of arachidonic acid in the vegulation of pregbnenolone synthesis in bovine adrenocortical mitchondria." J.Biochem.116. 833-837 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Naoko Morinaga et al.: "Differentiation of HL-60 cells is prometed by H-toxin of Clostridium septicum." FEBS Lett.351. 317-320 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mitsuo Nagase et al.: "Primiting effect of hydroxyapatite on the chemiluminescence resoponse in human polymorphonuclear leukocytes." FEBS Lett.349. 13-16 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masatoshi Noda,Joel Moss et al.: "Bacterial toxins and virulence factors in disease" Marcel Dekker,Lnc,New York, 627 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 野田公俊 竹田美文: "コレラ菌感染症の免疫学" 国際医書出版 東京, 504 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 野田 公俊: "MRSAの病原因子" Current Concepts in Infections Diseases. 13. 16-17 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 野田 公俊: "MRSAの毒素について" Today's Therapy. 18. 23-30 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 野田 公俊: "コレラ菌の毒素" Medical Immunology. 27. 407-414 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Morinaga: "Differentiation of HL-60 cells is promoted by H-toxin" FEBS Letters. 351. 317-320 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Nishikawa: "Chorela toxin can ADP-ribosylate,Gs as well as Gi" Endocrine Journal. 41. 593-597 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Nishikawa: "Possible involvement of lipoxygenese Metabolites" J.Biochem.116. 833-837 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 野田 公俊: "Clean Technology" 日本工業出版, 80 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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