Project/Area Number |
06454207
|
Research Category |
Grant-in-Aid for General Scientific Research (B)
|
Allocation Type | Single-year Grants |
Research Field |
Bacteriology (including Mycology)
|
Research Institution | KYUSHU UNIVERSITY |
Principal Investigator |
AMAKA Kazunobu KYUSHU UNIVERSITY,FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (20078752)
|
Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Shuji KYUSHU UNIVERSITY,FACULTY OF MEDICINE,LECTURER, 医学部, 講師 (30199369)
|
Project Period (FY) |
1994 – 1995
|
Project Status |
Completed (Fiscal Year 1995)
|
Budget Amount *help |
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥3,800,000 (Direct Cost: ¥3,800,000)
|
Keywords | Video tracking method / Bacterial motility / Swimming velocity of the bacteria / ビデオトラッキング / 走化性 / 定着因子 / 腸管定着 / ビデオトラッキング法 |
Research Abstract |
The patterns and the velocity of the bacterial movement by flagella were analyzed by the use of video tracking techique. The bacteria cultured in liquid medium was examined withe a dark field microscope and the images of the moving bacteria were incorporated into a image analyzing computer through the black and white video camera equipped in a microscope. The original images and the computed moving track were recorded in a video tape. The images on the tape were play back and sowe of the images were printed with a video printer. From the printed imapes of the bacterial movement we calculated the average of the swimming velocity of the bacteria and the moving patterns of the bacteria. The results showed that Vibrio cholerae move faster than the Pseudomonas aeruginosa. The movement of Salmonella was slower that the Pseudomonas.
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