Isolation and characterization of enterovirus 70 receptor molecule
| Project/Area Number |
06454214
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | The National Institute of Health, Japan |
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Principal Investigator |
TAKADA Naokazu National Institute of Health Department of Epidemiology Chief, 感染症疫学部, 室長 (90132894)
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| Co-Investigator(Kenkyū-buntansha) |
TATSUMI Masashi National Institute of Health Department of Animal Sciences Senior Researcher, 獣医科学部, 主任研究官 (00133629)
阪井 弘治 国立予防衛生研究所, エイズ研究センター, 主任研究官 (60260270)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | enterovirus / receptor |
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| Research Abstract |
Entrovirus 70 (EV70), a causative agent of acute haemorrhagic conjunctivitis, is a member of the genus enterovirus which comprises of 68 serotypes.To study EV70-cell interaction we have produced a monoclonal antibody that is capable of blocking EV70 infection by injecting the membrane fration from HeLa cells to BALB/c mice. The inhibition titer was 1.6 x 10^4 when 10^3 HeLa cells ware infected with 100 TCID_<50> EV70 in the presence of the antibody.The inhibition was similarly observed in FL,HEL and Hep2 cells but not in RD-18S, LIC-MK2 and RK13 cells (EV70 is an unusual enterovirus because of its capability to infect not only primate cells but also non-primate cells) .The inhibition assay using several enteroviruses confirmed the specificity of the antibody.Furthermore, the antibody was found to have an ability to block EV70 attachment to the susceptible cells.The immunofluorescence staining showed that the molecule is found to be located on the surface of the cells.We tried to isolate the molecule recognized by the monoclonal antibody.COS cells were transfected with cDNA libraty derived from HeLa cell mRNA.The so-called "panning" method was employed for the selection.The molecule was found to have the nucleotide sequence identical to that of the molecule which regulates a function of an immunologically important molecule.
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Report
(3 results)
Research Products
(7 results)
