| Budget Amount *help |
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1995: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Research Abstract |
Allergic contact dermatisi is mediated through a cell-immune responses and are generally observed among the certain occupations due to exposure to some reactive chemicals. For prevention of occupational allergic contact dermatitis, It is important to predict the contact sensitizing potentials to chemicals. This study was aimed at developing the methods for predicting contact sensitivity to chemicals using cytokine profile analysis. Balb/C femal mice were topically sensitized with DNCB,OXAZ and PCL,respectively, then allergic contact dermatitis was induced by the same sensitizer at non-irritant dose. The cytokine mRNAs at lymph organs and diseased skin sites were examined by reverse-trancription-polymerase chain reaction both in induction and challenge phases. The results have demonstrated that, at induction phase, only weak mRNA signals for IL-2, IL-4, IL-10, Ilp35, IL-12p40 and IFN-gamma were observed the draining lymph nodes whereas these cytokine mRNAs were strongly enhanced following sensitization with DNCB and OXAZ.Moreover, though the mRNAa of IL-5 and IL-13 were undetectable, but they were induced following treatment with DNCB and OXAZ.Furthermore, it was found that, at challenge phase, the expressions of IL-12 and IFN-gamma mRNAs were up-regulated in mice with allergic contact dermatitis whereas IL-2, IL-4 and IL-10 mRNAs remains unchanged. Although the mRNAs for IL-2, IL-4 and IFN-gamma were weakly found or undetectable at normal murine skin, they were largely augmented at the diseased skin sites. Therefore, our results suggest that simutanous dtection of cytokine profiles at skin and lymph nodes may be useful for indentify the contact sensitivity to occupational chemicals.
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