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Analyzes of accumulating T cell clonotypes in different joint lesions of rheumatoid arthritis

Research Project

Project/Area Number 06454253
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionKyushu University (1995)
St. Marianna University School of Medicine (1994)

Principal Investigator

YAMAMOTO Kazuhiko  Medical Institute of Bioregulation, Department of Clinical Immunology, Profesor, 生体防御医学研究所, 教授 (80191394)

Co-Investigator(Kenkyū-buntansha) SHIOKAWA Satoshi  Medical Institute of Bioregulation, Department of Clinical Immunology, Lecturer, 生体防御医学研究所, 助手 (20215940)
間藤 卓  東京大学, 医学部, 助手
加藤 智啓  聖マリアンナ医科大学, 難病治療研究センター, 助手 (80233807)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1995: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1994: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsT cell / T cell receptor / T cell clone / rheumatoid arthritis / PCR / SSCP / クロノタイプ
Research Abstract

we have established a novel method to analyze T cell clonality using a combination of reverse transcriptase-polymerase chain reaction of T cell receptor beta chain transcripts and single strand conformation polymorphism (SSCP). We then obtained synovial samples from patients during a therapeutic synovectomy for a T cell clonality analysis. An analysis of these samples revealed that synovial fluid samples and synovial tissue samples contained even more distinct accumulations of T cell clones. This indicates that there are antigen specific T cell reactions in the lesion of RA.We then compared the T cell clonalities in the different areas within the same joint. It was found that 60 to 95% of the accumulating T cell clones identifiedin one part of a joint also existed in another part of the same joint. The DNA sequencing of representative bands also confirmed this finding. Therefoer, the immunereactions of the RA lesions would be directed to limited common anrigens. Theidentities of the T cell clonalities in the lesions from different joints were also obseved at high frequencies, suggesting that pathogenicimmunereactions are commonamong the multiple joints of a RA patient.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Komagata, Y.: "Clonal prevalence of T cells infiltrating into the pancreas of non obese diabetic (NOD) mouse with the progressionof insulitis." Int. Immunol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okubo, M.: "T cell epitope mapping of U1-A ribonucleoprotein." Arthritis. Rheum.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ikeda, Y.: "High frequencies of identical T cell clonotypes in synovial tissues of rheumatoid arthritis patients suggest the occurrence of common antigen-driven immune respones" Arthritis Rheum.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masuko, K.: "Stable clonal expansion of cells induced by bone marrow transplantation." Blood.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamamoto, K.: "High frequencies of identical T-cell clonotypes accumulating in different areas of synovial lesions of rheumatoid arthritis patients." Proc. N. Y. Acad. Sci.756. 208-210 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamamoto, K.: "Accumulation of distinct T cell clonotypes in human solid tumors" J. Immunol.154. 1804-1809 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Komagata, Y.: "Clonal prevalence of T cells infiltrating into the pancreas of non obese diabetic (NOD) mouse with the progression of insulitis." Int.Immunol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okubo, M.: "T cell epitope mapping of Ul-A ribonucleoprotein." Arthritis.Rheum.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ikeda, Y.: "High frequencies of identical T cell clonotypes in synovial tissus of rheumatoid arthritis patients suggest the occurrence of common antigen-driven immune responses." Arthitis Rheum.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masuko, K.: "Stable clonal expansion of cells induced by bone marrow transplantation." Blood. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamamoto, K.: "High frequencies of identical T-cell clonotypes accumulating in different areas of synovial lesions of rheumatoid artheitis patients." Proc.N.Y.Acad.Sci.756. 208-210

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamamoto, K.: "Accumulation of distinct T cell clonotypes in human solid tumors." J.Immunol. 154. 1804-1809

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Komagata, Y.: "Clonal prevalence of T cells infiltrating into the pancreas of non obese diabetic (NOD) mouse with the progression of insulitis." Int. Immunol.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Okubo, M.: "T cell epitope mapping of Ul-A ribonucleoprotein." Arthritis. Rheum.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Ikeda, Y.: "High frequencies of identical T cell clonotypes in synovial tissues of rheumatoid arthritis patients suggest the occurrence of common antigen-driven jmmune responses" Arthritis. Rheum.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Masuko, K.: "Stable clonal expansion of cells induced by bone marrow transplantation." Blood.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamamoto, K.: "High frequencies of identical T-cell clonotypes accumulating in different areas of synovial lesions of rheumatoid arthritis patients." Proc. N. Y. Acad. Sci.756. 208-210 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamamoto, K.: "Accumulation of distinct T cell clonotypes in human solid tumors" J. Immunol.154. 1804-1809 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Dohi M,Yamamoto K,et al: "Accumulation of multiple T cell clonotypes in lungs of healthy individuals and patients with pulmonary sarcoidsis." J Immunol.152. 1983-1988 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yagi J,Nakata M,et al.: "Suporantigen-like proporties of the bispecific antibody Specific for MHC class II molecule and Vβ domain of the T cell antigen receptor." J Immunol.153. 3833-3841 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Okubo M,Kurokawa M,et al: "Clonotype analysis of the peripheral blocd T cells and the antoantigen-teactive T cells from patients with wixed connective tissue disarse." J Immunol.153. 3784-3790 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kato T,SuZuKi.S,et al.: "Compatison of the Jβ gene among differenf T cell receptor,Vp famihesin spleens of C57BL/6 mice." Eur.J.Immunol.24. 2410-2414 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miura H,Yamanaka H,etal.: "Iden fification of commonly recognized epito pes on a rare autoantigen:poly (ADP-ribose)polymerase." Clin.Immunol.Immunopathol.73. 290-295 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masuko K,Kato T,et al.: "Dynamic changes of accumulaied T cell clonotypes during antigenic stimulaticns in vivo and in vitro." Int Immunol. 6. 1959-1966 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kato T,SaSakawa H,et al.: "Autoepitopes of the SS/A(RO) KD molecule." Arthritis Rheuim.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto K,Aoro H,et al.: "Oligoclonal Proliferation of human T-cell leukemia Virus typeI in fected.lymphocytes in lesions of virus-induced arthropathy." Bio.Bio Res Commun.(in press).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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