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Molecular and cell biological investigation about the healing mechanism of gastric mucosal damage.

Research Project

Project/Area Number 06454266
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionJuntendo University School of Medicine

Principal Investigator

SATO Nobuhiro  Juntendo Univ.School of Medicine, Professor, 医学部, 教授 (90028358)

Co-Investigator(Kenkyū-buntansha) MAEHIRO Kohei  Juntendo Univ.School of Medicine, 医学部, 助手 (30238878)
YAMAGUCHI Yasushi  Juntendo Univ.School of Medicine, 医学部, 助手 (70241015)
KITAMURA Tsuneo  Juntendo Univ.School of Medicine, 医学部, 助手 (20231285)
WATANABE Sumio  Juntendo Univ.School of Medicine, Assistant Professor, 医学部, 助教授 (20138225)
横井 幸男  順天堂大学, 医学部, 講師 (20150633)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1994: ¥6,000,000 (Direct Cost: ¥6,000,000)
Keywordsgastric ulcer / growth factor / cytoskeleton / migration / proliferation / wound healing / culture cell / gastric ulcer / growth factor / cytoskeleton / migration / proliferation / wound healing / culture cell / gastric ulcer 男性度 / growth factor 増殖因子 / cytoskeleton 細胞骨性 / migration 細胞遊走 / proliferation 細胞増殖 / wound healing 損傷性対 / culture cell 培養細胞 / 胃粘膜細胞 / 損傷修復モデル / 細胞外基質 / 受容体
Research Abstract

The precise cellular mechanism of growth factors on gastric wound repair is still controversial. We investigated the role of growth factors on gastric mucosal restoration using primary cultured rabbit gastric epithelial cells and fibroblasts. Isolated rabbit gastric epitherial cells and fibroblasts were prepared from identical rabbit and cultured in F-12 medium. Inoculated epithelial cells (92% mucous cell) formed complete monolayr in 48h and fibroblasts formed complete monolayr in 4 weeks. A wound with cell-freearea of constant size was created by mechanical cell denudation using rotating silicon tip. The restoration process was monitored by measuring wound size every 12 h using a time-lapse video system. The proliferative cells were detected by serial staining for BrdU.Effects of EGF, Insulin, bFGF, HGF, TGFalpha and PDGF were assessed. EGF, Insulin, HGF, TGFalpha and PDGF accelerated the gastric epithelial restoration with the promotion of cell migration and proliferation. bFGF and PDGF accelerated the gastric mesenchymal restoration. The difference of target cells of growth factor might modulate gastric ulcer healing and the integrity of growth factor network during the process of ulcer healing is essential for high quality ulcer healing.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Watanabe S,Wang X,Kitamura T,Sato N: "Basic fibroblast growth factor accelerates gastric mucosal restration in vitro by promoting mesenchymal cell migration and proliferation." J of Gastroenterology and Hepatology. 10. 627-632 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe S,Hirose M,Maehiro K,Sato N: "A new model to study repair of gastric mucosa using primary cultured rabbit gastric epithelial cells." J Clinical Gastroenterol. 21. 40-44 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ikejima K,Watanabe S,Miyazaki A,Sato N.: "Hepatocyte growth factor inhibits interoellular communication via gap junction in rat hepatocytes." Biochemical & Biophysical Research Communication. 214. 441-446 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Endo K,Watanabe S,Nagahara A,Sato N.: "Restoration of gap junctions in the regenerative process of ethanol induced gastric mucosal injury." J of Gastroenterology and Hepatology. 10. 589-594 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe S,Hirose M,Miyazaki A,Sato N.: "Role of actin and calmodulin in migration and proliferation rabbit gastric mucosal cells in culture." J of Gastroenterology and Hepatology. 9. 325-333 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato N,Watanabe S,Hirose M,Ogihara T.: "Effect of nicotine in migration and proliferation of rabbit gastric mucosal cells in a culture cell model." J of Gastroenterology and Hepatology. 9. 66-71 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe S, Wang X, Kitamura T, Sato N.: "Basic fibroblast growth factor accelerates gastric mucosal restoration in vitro by promoting mesenchymal cell migration and proliferation." J of Gastroenterolgy & Hepatology. 10 :. 627-632 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe S, Hirose M, Maehiro K, Sato N.: "A new model to study repair of gastric mucosa using primary culture rabbit gastric epithelial cells." J Clinical Gastroenterol. 21 :. 40-44 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ikejima K, Watanabe S, Miyazaki A, Sato N.: "Hepatocyte growth factor inhibits intercellular communication via gap junction in rat hepatocytes." Biochemical and Biophysical Research Communication. 214 :. 441-446 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Endo K, Watanabe S, Nagahara A, Sato N.: "Restoration of gap junctions in the regenerative process of ethanol induced gastric mucosal injury." J of Gastroenterology and Hepatology. 10 :. 589-594 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe S, Hirose M, Miyazaki A, Sato N.: "Role of actin and calmodulin in migration and proliferation of rabbit gastric mucosal cells in culture." J of Gastroenterology and Hepatology. 9 :. 325-333 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato N, Watanabe S, Hirose M, Ogihara T.: "Effect of nicotine in migration and proliferation of rabbit gastric mucosal cells in a culture cell model." J of Gastroenterology and Hepatology. 9 :. 66-71 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe S, Wang X, Hirose M, Sato N.: "Platelet derived growth factor accaelerates gastric epithelial restoration in a rabbit culture cell model." Gastroenterology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato N, Kawano S, Tsuji S, Ogihara T.: "Gastric blood flow in ulcer diseases." Scand J of Gstroenterology. 30 :. 14-20 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe S, Wnag X, Hirose M, Miyazaki A, Sato N.: "Integrity of myosin light chain kinase is essential for Ito cell contraction." J of Gastroenterlogy and Hepatology. 10 :. 54-56 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe S, Wang X, Kitamura T, Sato N: "Basic fibroblast growth factor accelerates gastric mucosal restoration in vitro by promoting mesenchymal cell migration and proliferation." J of Gastroenterology and Hepatology. 10. 627-632 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Watanabe S, Hirose M, Maehiro K, Sato N: "A new model to study repair of gastric mucosa using primary cultured rabbit gastric epithelial cells." J Clinical Gastroenterol. 21. 40-44 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ikejima K, Watanabe S, Miyazaki A, Sato N: "Hepatocyte growth factor inhibits intercellular communication via gap junction in rat hepatocytes." Biochemical and Biophysical Research communication. 214. 441-446 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Endo K, Watanabe S, Nagahara A, Sato N: "Restoration of gap junctions in the regenerative process of ethanol induced gastric mucosal injury." J of Gastroeterology and Hepatology. 10. 589-594 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Watanabe S, Hirose M, Miyazaki A, Sato N: "Role of actin and calmodulin in migration and proliferation of rabbit gastric mucosal cells in culture." J of Gastroenterology and Hepatology. 9. 325-333 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato N, Watanabe S, Hirose M, Ogihara T,: "Effect of nicotine in migration and proliferation of rabbit gastric mucosal cells in a culture cell model." J of Gastroenterology and Hepatology. 9. 66-71 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sumio Watanabe: "Effect of myosin light chain kinase inhibitor wortmannin on the wound repair of cultured gastric mucosal cells." Biochem Biophys Res Comm. 199. 799-806 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sumio watanabe: "Hepatocyte growth factor a ccelerates the wound repair of cultured gastric mucosal cells" Biochem Biophys Res Comm. 199. 1453-1460 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sumio Watanabe: "Role of actin and calmodulin in migration and proliferation of rabbit gastric mucosal cells in culture." J Gastroenterol Hepatology. 9. 325-333 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nobuhiro Sato: "Effect of nicotine in migration and proliferation of rabbit gastric mucosal cells in a culture cell model." J Gastroenterol Hepatology. 9. S66-S71 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hideaki Mikami: "Role of extracellular matrix in wound repair by cultured gastric mucosal cells." Biochem Biophys Res Comm. 202. 285-292 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Keiichiro Endo: "Restoration of gap iunctions in the regenerative process of ethanol-induced gastric mucosal injury" J Gastroenterol Hepatology. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 渡辺純夫.佐藤信紘: "胃-形態とその機能" 医学書院, 354 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤信紘: "日本消化性潰瘍学" 日本消化看病学会附属研究会編(医科学出版社)(in press), (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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