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Basic research for the Wilson's disease gene therapy

Research Project

Project/Area Number 06454304
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionNagoya City University

Principal Investigator

WADA Yoshiro  Nagoya City University, School of Medicine, Professor, 医学部, 教授 (30004849)

Co-Investigator(Kenkyū-buntansha) AGUI Takashi  Nagoya City University, School of Medicine, Associate Professor, 医学部, 助教授 (00212457)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsWilson's desease / Gene therapy / LEC rats
Research Abstract

Restriction fragment length polymoyphism on the Wilson's disease gene betweem LEC and control WKAH rats was found by using a human Wilson's disease cDNA fragment amplified with polymerase chain reaction as the probe. This RFLP was found to show a cross-linkage with the hepatic disorder in back-crosses between LEC and WKAH rats, indicating that the hepatic disorder in LEC rats is due to the same gene as the case of human Wilson's disease. This result suggests that LEC rats are usuful animal model for the Wilson's disease gene therapy.
In order to construct a retro virus vector inserted the human Wilson's disease gene for the gene therapy, we tried cloning of this gene cDNA from the cDNA library made from the healthy human liver. However, cDNA clones obtained all did not encode the whole coding region, possibly due to the relatively long coding region of this gene. We are under re-screening the cDNA library to obtain cDNA encoding the whole coding region.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] N. Sasaki et al.: "The gene responsible for LEC hepatitis,located on rat chromosome 16,is the homolog to the human Wilson disease gene." Biochem. Biophys. Res. Commun.202. 512-518 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Sasaki et al.: "The gene responsible for LEC hepatitis, located on rat chromosome 16, is the homolog to the human Wilson disease gene." Biochem.Biophys.Res.Commun.202. 512-518 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Sasaki et al.: "The gene responsible for LEC hepatitis,licated on rat chromosome 16,is the homolog to the human Wilson disease gene" Biochem.Biophys.Res.Commun.202. 512-518 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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