• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of cause of Kawasaki disease, molecular infecto-immunolgical analysis

Research Project

Project/Area Number 06454310
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionKurume University

Principal Investigator

KATO Hirohisa  Kurume University, Medicine, Professor, 医学部, 教授 (30080724)

Co-Investigator(Kenkyū-buntansha) SAKAGUCHI Minako  Kurume University, Medicine, Assisant Professor, 医学部, 助手 (50258422)
NISHIYORI Atsushi  Kurume University, Medicine, Assistant Professor, 医学部, 助手 (30218226)
SATO Noboru  Kurume University, Medicine, Assistant Professor, 医学部, 助手 (50205952)
SAGAWA Kimitaka  Kurume University, Medicine, Professor, 医学部, 教授 (20140650)
杉村 徹  久留米大学, 医学部, 助手 (80248400)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1994: ¥5,700,000 (Direct Cost: ¥5,700,000)
KeywordsKawasaki disease / T cell receptor / super antigen / TNF-α
Research Abstract

The etiology of KD is unknown. Some reports indicated that KD is associated with the selective expansion of Vbeta2^+ and Vbeta8.1^+T cells in peripheral blood lymphocytes (PBL). To understand better the ethiology of KD,we investigated T cell receptor (TCR) Vbeta^<2+> and Vbeta8.1^+ expansion on the T cell of freshly isolated PBL and T cell clones (TCC). Blood samples were obtained from patients with acute (n=20) and convalescent (n=20) KD,age matched children with non-infectious disease (n=18), and healthy adults (n=20). Among these four groups, there were no significant differences in the percentages of their Vbeta^<2+> and Vbeta8.1^+T cells. The same was true for the CD4^+ or CD8^+ T cell subsets. One hundred and five TCC established from the affected skin, lymphnode or PBL of six patients with KD were also negative for either Vbeta2 or Vbeta8.1 TCR.Sixty-eight of 105 TCC produced detectable levels of TNF-alpha, in the absence of any stimuli. In contrast, only 11 or 7 produced detectable levels of IL-2 or IL-6, respectively. Stimulation with phytohaemagglutinin and phorbol myristate induced most TCC to produce higher amounts of TNF-alpha, IL-2 and IL-6. These results suggest that CD4^+ T helper cells expressing TCR-beta other than Vbeta2 or Vbeta8.1 receptor, primarily through TNF-alpha production, are involved in the immunopathology of KD.In other reports that KD is associated with the selective expansion of Vbeta2 and Vbeta8.1, in vitro activated PBL were used. Therfore, their method may allow possibly allow in vitro modification of TCR usage.
Fouthermore we measured TSST-1 concentrations and anti-TSST-1 antibody in serum from KD patients in both the acute and the convalescent phase. TSST-1 was reported as a possible cause of KD recently. Bur we failed to find any evidence that KD is caused by TSST-1.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Sakaguchi M: "Characterization of CD4^+ T helper cells in patients with Kawasaki disease." Clin Exp Immunol. 99. 276-282 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yanagawa H: "Epidemiologic pictures of Kawasaki disease in Japan." Pediatrics. 95. 475-479 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yanagawa H: "Results twelve nationwide epidemiological incidencesurveys of Kawasaki disease in Japan." Arch Pediatr Adolesc Med. 149. 779-783 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishiyori A: "KAWASAKI DISEASE" EXCERPTA MEDICA, 5 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakaguchi M: "KAWASAKI DISEASE" EXCERPTA MEDICA, 7 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugimura T: "Intravascular ultrasound of coronary arteries in children." Circulation. 89. 258-265 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishiyori A: "Toxic shock syndrome toxin-secreting Staphyrococcus aureus in Kawasaki disease." Lancet. 343. 299-300 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakamura Y: "Incidence rate of recurrent Kawasaki disease in Japan." Acta Pediatrica. 83. 1061-1064 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato H: "Kawasaki disease" Coronary Artery Dis. 6. 194-206 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakaguchi M: "Characterization of CD4^+T helper cells in patients with Kawasaki desease : preferential production of tumor necrosis factor-alpha (TNF-alpha) by Vb2 or Vb8 CD4^+T helper cells." Clin Exp Immunol. 99. 475-479 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yanagawa H: "Epidemiologic pictures of Kawasaki disease in Japan." Pediatrics. 95. 475-479 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yanagawa: "Results twelve nationwide epidemiological incidences surveys of Kawasaki disease in Japan." Arch Pediatr Adolesc Med. 149. 779-783 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishiyori A: "KAWASAKI DISEASE." EXCERPTA MEDICA. 5. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakaguchi M: "KAWASAKI DISEASE." EXCERPTA MEDICA. 7. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato N: "KAWASAKI DISEASE." EXCERPTA MEDICA. 7. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato N: "KAWASAKI DISEASE." EXCERPTA MEDICA. 10. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugimura T: "KAWASAKI DISEASE." EXCERPTA MEDICA. 6. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Akagi T: "KAWASAKI DISEASE." EXCERPTA MEDICA. 5. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato H: "KAWASAKI DISEASE." EXCERPTA MEDICA. 10. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugimura T: "KAWASAKI DISEASE." EXCERPTA MEDICA. 6. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato H: "Kawasaki disease" Coronary Artery Dis. 6. 194-206 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sakaguchi M: "Characterization of CD4^+T helper cells in patients with Kawasaki disease." Clin Exp Immunol. 99. 276-282 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yanagawa H: "Epidemiologic pictures of Kawasaki disease in Japan." Pediatrics. 95. 475-479 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yanagawa H: "Results twelve nationwide epidemiological incidences incidences surveys of Kawasaki disease in Japan." Arch Pediatr Adolesc Med. 149. 779-783 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishiyori A: "Kawasaki disease" EXCERPTA MEDICA, 5 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sakaguchi M: "Kawasaki disease" EXCERPTA MEDICA, 7 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishiyori A.: "Toxic shock syndrome toxin-secreting Stophylococous aureus in Kawasaki syndrome" Lancet. 343. 299-300 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.SAKAGUCHI: "Characterization of CD4^+T helper cells in patients" Clinical Experimental Immunology. 99. 276-282 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tetsu Sugimura: "Intravascular Ultrasound of Coronary Arteries in Children" Circulation. 89. 258-265 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 加藤裕久: "成人になった川崎病-川崎病の自然歴と長期的問題-" 小児科診療. 57(12号). 2279-2285 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 加藤裕久: "川崎病血管炎" 日本臨牀. 52(8号). 2095-2102 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi