• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A cell bank system for cultured epithelial sheet transplantation

Research Project

Project/Area Number 06454317
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionOsaka University

Principal Investigator

YOSHIKAWA Kunihiko  Osaka Univ.Dept.of Derma.Professor, 医学部, 教授 (20110851)

Co-Investigator(Kenkyū-buntansha) KAKIBUTI Masao  Osaka Univ.Dept.of Deram.Assistant Professor, 医学部, 助手 (50252664)
HIGASHIYAMA Mari  Osaka Univ.Dept.of Deram.Assistant Professor, 医学部, 助手 (40228713)
HASHIMOTO Kozi  Ehime Univ.Dept.of Deram.Professor, 医学部, 教授 (00110784)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsCultured epithelial sheet / Transplantation / Ultra-deep freezer / Epiderm cells / Leg ulcer / Epidermolysis bullosa / Burn / Cell bank / 表皮ケラチノサイト / 培養表皮シート移植 / 細胞バンク / 培養センター / L-15
Research Abstract

1)Development of preservation and transportation method for biopsied skin and cultured epithelial sheet. For incorporating cultured epithelial sheet technique into Japanese medical care system, it is necessary to establish culture center for supplying cultured epithelial sheet. We examined the best medium to keep biopsied skins for 4 days under refrigerated condition. We found that L-15 medium is suitable for this purpose. Next, we studied how long cultured epithelial sheet could be stored in favorable condition after detachment from culture plate. Cultured epithelial sheet could be preserved in good condition under refrigerated condition with Hanks solution for 12 hours, but not foe 24 hours. This result led us to develop a new method to preserve cultured epithelial sheet under frozen condition. We found that L-15 medium containing polyethylene glycol 600 is the best medium for this purpose. Cultured epithelial sheet could be frozen and stored in ultra-deep freezer (-150゚C) for up to … More 3 months. Furthermore, storage in dry ice for 72 hours did not cause any unfavorable damage to cultured epiderm sheet. We also confirmed that cultured epithelial cells could be stored in liquid nitrogen for over 20 years theoretically. 2)Assessment of clinical application of cultured epithelial sheet. First, we performed the compared analysis for the effectiveness of frozen and non-frozen cultured epithelial sheet. There were no significant difference between both of them. Based on this result, we used frozen autograft cultured epithelial sheet in general. We tried clinical application of cultured epithelial sheet mainly for recurrent leg ulcer, epidermolysis bullosa and sever burn. The marked improvement was observed in epidermolysis bullosa, in which durati on of ulcer epihtelization was markedly shortened and blister formation due to mechanical force was decreased in transplanted lesions. In leg ulcer, repetitive transplantation of cultured epithelial sheet was performed using frozen-stored epidermal keratinocytes. Thus, the possibility of cell bank system in cultured epithelial sheet transplantation was suggested. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 垣淵正男: "皮膚培養法による自家移植" 病態生理. 13. 529-533 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 細川 亙: "皮膚移植による最近の進歩" 日本医事新報. 3681. 27-30 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 橋本公二: "培養表皮シート移植" 皮膚病診療. 17. 432-436 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hashimoto K et al: "Heparin-binding epidermal growth dactor-like growth factor is an autocrine growth factor for numan keratinocytes" Journal Biological Chemistry. 269. 20060-20066 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wataya-Kaneda M ey al: "Diffkrential locariztion of TGF-beta-precursor isotypes in normal human skin" Journal of Dermatological Science. 8. 38-44 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Higashiyama M et al: "Differential expression of transforming growth factor α (TGF-α) and receptor in transitional area of psoriatic epidermis" Journal of Dermatological Science. 7. 45-53 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 吉川邦彦: "皮膚の健康科学(表皮細胞における情報遺伝機構)" 南山堂, (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi T,et al: "Dephosphorylation of the retinoblastoma gene product induced by differentiation and its relevancy to growth inhibition in normal human keratinocytes." J.Dermatol.Sci.8. 171-177 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hashimoto K,et al: "Heparin-binding epidermal growth factor-like growth factoe is an autocrine growth factor for human keratinocytes." J.Biol.Chem.269 (39). 20060-20066 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wataya-Kaneda M,et al: "Differential localization of TGF-beta-precursor isotypes in normal human skin." J.Dermatol.Sci.8 (1). 38-44 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takada A,et al: "Growth-promoting effect of bacterial products from clostridium perfringens on human keratinocytes" J.Dermatol.21. 539-545 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mizutani H,et al: "Functional and immunoreactive thrombomodulin expressed by keratinocytes" J.Invest.Dermatol.(1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi T,et al: "Effect of donor age on the phosphorylation of the retinoblastoma gene product in cultured human keratinocytes" J.Dermatol.Sci.9 (1). 59-63 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hashimoto K: "Growth regulation of epidermis by growth factors-two aspects of their function" J.Jap.Drmatol.Asso.104. 1632-1634 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hashimoto K: "Discovery of new functions of vitamin and biofacto (10) psoriasis and vitamin D" Vitamin. 69. 33-38 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hashimoto K: "Cultured epidermal sheet graft-dream of cell bank system" Hifubyoh-Shinryyoh. 17. 432-436 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi T,et al: "Growth inhibition of human keratinocytes by MC903J.(Calcipotrioal) is linked to dephosphorylation of retinoblastoma geneproduct" Eur.Aca.Dermatol.Venerol.5. 132-138 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 垣淵正男: "皮膚培養法による自家移植" 病態生理. 13. 529-533 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 細川 亙: "皮膚移植による最近の進歩" 日本医事新報. 3681. 27-30 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 橋本公二: "培養表皮シート移植" 皮膚病診療. 17. 432-436 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hashimoto K et al: "Heparin-binding epidermal growth factor-like growth factor is an autocrine growth factor for human keratinocytes" Journal Biological Chemistry. 269. 20060-20066 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wataya-Kaneda M et al: "Differential locarization of TGF-beta-precursor isotypes in normal human skin" Journal of Dermatological Science. 8. 38-44 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Higashiyama M et al: "Differential expression of transforming growth factor-α (TGF-α) and receptor in transitional area of psoriatic epidermis" Journal of Dermatological Science. 7. 45-53 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 吉川邦彦: "皮膚の健康科学(表皮細胞における情報遺伝機構)" 南山堂, (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 垣淵正男、吉川邦彦: "皮膚培養法による自家移植" 病態生理. 13. 529-533 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 垣淵正男、吉川邦彦: "培養表皮シートを用いる皮膚移植" 総合臨床. 43. 45-47 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi