| Project/Area Number |
06454375
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Keio University |
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Principal Investigator |
KOBAYASHI Koichi Keio University, General Thoracic surgery, Surgery, Professor, 医学部, 教授 (80051704)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAHATA Takeshi Keio University, general Thoracic surgery, Surgery, Assistant, 医学部, 助手 (10255482)
IZUMI Yotaro Keio University, General Thoracic surgery, Surgery, Assistant, 医学部, 助手 (90245506)
KIKUCHI Koji Keio University, General Thoracic surgery, Surgery, Lecture, 医学部, 講師 (40129408)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | artificial oxygen carrier / totally synthetic oxygen carrier / red blood cell substitute / lipid heme microsphere / exchange transfusion / 脱血交換実験 |
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| Research Abstract |
Totally synthetic oxygen carrying substance (artificial red cell) was produced by covering clinically available fat droplets (triglyceride microsphers) with synthetic lipidhemes. Thus fat droplets were used as vehicles of lipidhemes as oxygen carrier. The size of this artificial red blood cell substitute is 103 <plus-minus> 29nm. Specific gravity, viscosity and osmotic pressure were very similar to that of human blood. In vitro study its oxygen binding was reversible and fast, the P50 was 40mmHg. Half life time in blood was 10-13 hour
Exchange transfusion in beagles upto 60% of circulating blood volume showed that oxygen transport to the tissue by this red blood substitute was about 50ml/min which corresponded about 40% of total oxygen transport. Oxygen consumption from this oxygen carrier was 25ml/min which corresponded about 25% of total oxygen consumption. In exchange transfusion upto 80%, it was difficult to keep beagles alive because of pulmonary edema due to severe hypoalbuminemia. To continue experiment it was necessaty to use total cardiopulmonary bypass. Another idea is to produce artificial oxygen carrier which has oncotic pressure. We are now producing this kind of material by using albumin in which lipidhemes are embedded. As perfusate albumin was mandatory to increase colloid oncotic pressure to this lipidheme microsphere. Albumin which has colloid oncotic pressure, is used as vehicles of lipid heme as oxygen carrier.
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