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Genetical analasys of genesis of multiple primary squamous cell carcinoma of lung

Research Project

Project/Area Number 06454394
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionTohoku University

Principal Investigator

SATO Masami  Tohoku University, Institute of Development, Aging and Cancer, assistant professor, 加齢医学研究所, 助手 (30250830)

Co-Investigator(Kenkyū-buntansha) SAITO Yasuki  Tohoku University Institute of Development, Aging and Cancer, assistant professo, 加齢医学研究所, 助手 (90153824)
FUKIMURA Shigefumi  Tohoku University Institute of Development, Aging and Cancer, professor, 加齢医学研究所, 教授 (40006078)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥4,100,000 (Direct Cost: ¥4,100,000)
Keywordsroentgenographically occult lung cancer / immunohistostain / tumor supperssor gene p53 / PCR-SSCP / bcl-2 / apoptosis / TUNEL method / cdk inhibitor p21 / PCP-SSCP法 / アポトーシス関連遺伝子bcl-2 / 肺扁平上皮癌 / p53 / 癌抑制遺伝子 / 発癌 / 多発癌 / 増殖動態
Research Abstract

We examined the relationship between lung cancer and aberration of p53 gene. By means of PCR-SSCP(single strand conformation polymorphism), the aberration of p53 gene was found in roentgenographically occult lung cancers as much as in advanced lung cancers. By immunohistostain, p53 protein positive rate had increased according to the development of cell atypia, and no p53 protein was found in normal and hyperplasia of bronchial epithelium. These things suggested that the aberration of p53 gene may occur in the stage of dysplasia of bronchial epithelium at first, but not only the aberration of p53 gene take part in carcinogenesis of lung. We must find aberration of other genes and make clear the carcinogenesis of lung.
And we examined the relationship between p53 protein and p21 protein, which is the cdk inhibitor induced by p53. Correlation between p21 and p53 was not found in immunohistostain. This was suggested p21 was not induced only p53 in lung cancer. By immunohistostain, p21 protein positive rate was higher in node-positive lung cancer, undifferentiated lung cancer and over stage II lung cancer. This suggested p21 may be related to extent of invasion and metastasis.
Finally, we immunohistochemically investigated the expression of bcl-2protein, Apoptosis and p53 protein in non-small cell lung carcinoma. The expression of bcl-2 protein, Apop Tag and p53 protein was higher in squamous cell carcinoma than the other histologic type. The survival rate was better among patients with bcl-2 positive. And the expression of bcl-2 was higher in the early stage of patients than in advances stage of patients. The exrpession of p53 protein was higher in the group with bcl-2 positive cases than in the group with bcl-2 negative cases. The expression of Apop Tag was singnificantly higher in the group with bcl-2 protein and p53 protein positive cases than the other ones. (p-0.018). These suggested a new pathway must exist in a course of apoptosis.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Yasuki Saito: "Multicenteicity Resected Occult Bronchogenic Squamous Cell Caricinoma" Ann Thorac Surg. 57. 1200-1205 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katsuo Usuda: "Tumor Doubling TIme and Prognostic Assessment of Patients with Primary Lung Cancer" Cancer. 74. 2239-2244 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐川元保: "PCR法によるI期(pT1N0M0)肺扁平上皮癌組織中のHPV 16, 18, 33型の検出" 胸部外科. 48. 360-362 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 薄田勝男: "原発性肺癌における核DNA量の解析" 日本胸部外科学会雑誌. 42. 486-491 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 薄田勝男: "原発性肺癌における核小体形成部位(AgNORs)の評価" 日本胸部外科学会雑誌. 42. 545-550 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasuki Saito: "Multicentricity in Resected Occult Bronchogenic Squamous Cell Carcinoma" Ann Thorac Surg. 57. 1200-1205 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katsuo Usuda: "Tumor Doubling Time and Prognostic Assessment of Patients with Primary Lung Cancer" Cancer. 74. 2239-2244 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Motoyasu Sagawa: "Detection of Human Papillomanirus Type 16,18,33 DNA in stage I (pTlN0M0) Squamous Cell Carcinoma of the Lung by Polymerase Chain Reaction" Jpn J Thorac Surg. 48. 360-362 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katsuo Usuda: "Assessment of Nucleolar Organizer Regions (Ag-NORs) in Primary Lung Cancer" J Jpn Assn Thorac Surg. 42. 545-550 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katsuo Usuda: "Flow Cytometric Analysis of Nuclear DNA Content of Lung Cancer" J Jpn Assn Thorac Surg. 42. 486-491 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasuki Saito: "Multicenteicity in Resected Occult Bronchogenic Squamous Cell Caricinoma" Ann Thorac Surg. 57. 1200-5 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Katsuo Usuda: "Tumor Doubling TIme and Prognostic Assessment of Patients with Primary Lung Cancer" Cancer. 74. 2239-44 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐川元保: "PCR法によるI期(pTINOMO)肺扁平上皮癌組織中のHPV16,18,33型の検出" 胸部外科. 48. 360-2 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 薄田勝男: "原発性肺癌における核DNA量の解析" 日本胸部外科学会雑誌. 42. 486-91 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 薄田勝男: "原発性肺癌における核小体形成部位(AgNORs)の評価" 日本胸部外科学会雑誌. 42. 545-50 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Saito Y: "Multicentricity in Resected Occult Bronchogenic Squamous Cell Carcinoma" Ann Thorac Surg. 57. 1200-1205 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 薄田勝男: "原発性肺癌における核小体形成部位(Ag-Nors)の評価" 日本胸部外科学会雑誌. 42. 69-74 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nagamoto N: "Growth Features of Roentgenographically Occult Exophytic Squamous Cell Carcinoma of the Lung" J Bronchol. 1. 26-30 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 薄田勝男: "原発性肺癌における核DNA量の解析" 日本胸部外科学会雑誌. 42. 10-15 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤雅美: "気管支早期扁平上皮癌および境界病変におけるp53蛋白の発現の検討" 肺癌. 34. 708 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 遠藤千顕: "PCR-SSCP法による肺扁平上皮組織における癌抑制遺伝子p53変異" 肺癌. 34. 709 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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