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RESEARCH ON HUMAN AIRWAY EPITHELIUM ACTING AS ALLOANTIGEN-PRESENTING CELLS

Research Project

Project/Area Number 06454397
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionUniversity of Tokyo

Principal Investigator

NAKAJIMA Jun  UNIVERSITY OF TOKYO,FACULTY OF MEDICINE,STAFF, 医学部(病), 助手 (90188954)

Co-Investigator(Kenkyū-buntansha) ONO Minoru  UNIVERSITY OF TOKYO,FACULTY OF MEDICINE,ASSOCIATE, 医学部(病), 助手 (40270871)
TAKEDA Makoto  UNIVERSITY OF TOKYO,FACULTY OF MEDICINE,ASSOCIATE, 医学部(病), 助手 (10236482)
KAWAUCHI Motohiro  UNIVERSITY OF TOKYO,FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部(病), 講師 (00152918)
FURUSE Akira  UNIVERSITY OF TOKYO,FACULTY OF MEDICINE,PROFESSOR, 医学部(病), 教授 (70010163)
大塚 俊哉  東京大学, 医学部(病), 助手
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥5,200,000 (Direct Cost: ¥5,200,000)
Keywordsairway epithelial cell line / BEAS-2B / allogeneic lymphocyte / mixed lymphocyte culture / interleukin-10 (IL-10) / LFA-3 / Th2 fraction / costimulatory factor / 気動上皮細胞 / 同種末梢血リンパ球 / リンパ球混合培養 / costimulatory facror / Th2 / ICAM-1 / B7 / 気道上皮細胞株 / リンパ球混合培養系 / 上清中濃度 / MTTassay / Interleukin-2 / Interleukin-10 / HLA-クラス2抗原
Research Abstract

In lung allotransplantation, circulating leukocytes and vascular endothelial cells have been considered to be antigen-presenting cells (APCs). First in this research, we determined whether airway epithelial cells function as APCs.An immortal human epithelial cell line, BEAS-2B (B2B,1*10^5 cells/ml), which constitutively expresses both HLA-class I and class II antigens along with costimulatory molecules, ICAM-1, LFA-3, and B7 on its surface, was irradiated (50Gy) and transferred to 10ml of complete culture medium with allogeneic human peripheral blood lymphocyte (PBL) (1*10^6 cells/ml) from a healthy volunteer. The mixed lymphocyte-epithelial cell line culture (ML-AECL) was then incubated for 3 days, and harvested. As a control study, a one-way mixed lymphocyte culture (MLC) was also made up. Concentration of interleukin (IL) -10 in each supeynatant were assayd using an EASIA method. The concentration of IL-10 was not elevatedin MLC (9.3<plus-minus>11.8pg/ml, N=5) but significantly elev … More atedin ML-AECL (128<plus-minus>110pg/ml, N=5, p=0.043). Stimulation of B2B induceda population of allogeneic lymphocytes to proliferate and secrete IL-10. It has been reported that Th2, a subset of helper-T cells, secretes IL-10, which in turn inhibits both Th1 and cytotoxic T cells (CTLs) from proliferating and becoming active. We suggest that airway epithelial cells stimulate Th2, and, as a result, may play a role is suppressiing the allospecific reaction in acute rejection.
Second, we investigated the role of costimulatory molecules on B2B.B2B were pretreated with either anti-HLA class I,anti-HLA class II,anti : ICAM-1, anti-LFA-3, or anti-B7 monoclonal antibody, Then the ML-AECL culture was then incubated, and harvested. Cell proliferation assay was performed using the colorimetric (MTT) assay. The MTT indeces (percentages relative to the control) were found to be significantly lower than the control when HLA class I,class II or LFA-3 on B2B was masked. In mixed culture consisting of B2B cells as stimulators and allogeneic lymphocytes, the lymphocytes recognized MHC-class I and II antigens expressed on the BEAS-2B.LFA-3 on BEAS-2B also contributed to the activation of lymphocyte alloreactivity. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Nakajima J, 他5名: "Characteristic findings of pulmonary arteriograophy in xenografted lung of the primates." Transplantation Proceedings. 27. 310-312 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakajima J, 他6名: "Human allogeneic airway epithelial cell line induced lymphocyte to secrete interleukin-10." Transplantation Proceedings. 28(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kawauchi M. Nakajima J, 他2名: "Cardiac xenotransplantation from pig to Japanese monkey with splenectomy, tacrolims, filtration plasmapheresis,and nafamstat mesilate." Transplantation Proceedings. 26. 1076-1077 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kawauchi M, Nakajima J, 他5名: "Which is the target vessel of lung rejection? A primate heart-lung transplantation study." Transplantation Proceedings. 26. 2338-2339 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kawauchi M, Nakajima J, 他3名: "Contribution of T cells in concordant xenoheart rejection." Transplantation Proceedings. 26. 1193-1194 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大岩博、中島淳、他3名: "FK506の血中及び臓器別濃度-ニホンザル肺移植における検討" 移植. 29. 144-148 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakajima J, Furuse A, Oka T, et al: "Excellent Survival in a subgroup of patients with intrapulmonary metastasis of lung cancer." Annals of Thoracic Surgery. 61. 158-163 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakajima J, Furuse A, et al: "Hemoptysis from emphysematous bulla following open heart surgery." Surgery Today. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jun Nakajima: "Problems in xenografted lung of the primates.(in Update clinical immunology,Molecular Science in Organ Transplantation)" Pabst Science Publishers,Lengerich,Germany(出版中), 20 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jun Nakajima, Akira Furuse, et al: "Characteristic findings of pulmonary arteriography in xenografted lung of the primates." Transplantation Proceedings. 27. 310-312 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jun Nakajima, Akira Furuse, et al: "Human allogeneic epithelial cell line inducedlymphocyte to secrete interleukin-10" Transplantation Proceedings. 28(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jun Nakajima, Akira Furuse, Teruaki Oka, et al: "Excellent Survival in a subgroup of patients with intrapulmonary metastasis of lung cancer." Annals of Thoracis Surgery. 61. 158-163 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jun Nakajima, Akira Furuse, et al: "Hemoptysis from emphysematous bulla following open heart surgery" Surgery Today. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Motohiro Kawauchi, Jun Nakajima Akira Furuse, et al: "Cardiac xenotransplantation from pig to Japanese monkey with splenectomy, tacrolims, filtration plasmapheresis, and nafamstat mesilate" Transplantation Proceeings. 26. 1076-1077 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Motohiro Kawauchi, Jun Nakajima, Akira Furuse, et al: "Which is the target vessel of lung rejection? A primate heart-lung transplantation study" Transplantation Proceedings. 26. 2338-2339 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Motohiro Kawauchi, Jun Nakajima, Akira Furuse, et al: "Contribution of T cells in concordant xenoheart rejection." Transplantation Proceedings. 26. 1193-1194 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroshi Oiwa, Jun Nakajima, Akira Furuse, et al: "[The distribution of FK506 in the blood and other organs on a non-human primate model of lung transplantation] (Japanese manuscript with English abstract)" Japanese Journal of Transplantation. 29. 144-148 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jun Nakajima: Problems in xenograftedlung of the primates. in Molecular biological methods and approaches in transplantation medicine. editedby Hammer C,Vogt S,Troitzsch D :. Pabst Science Publishers, Lengerich, Berlin, Germany (in press), (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakajima J.,他5名: "Characteristic findings of pulmonary arteriography in xenografted lung of the primates." Transplantation Proceedings. 27. 310-312 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakajima J.,他6名: "Human allogeneic airway epithelial cell line induced lymphocyte to secrete interleukin-10." Transplantation Proceedings. 28(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawauchi M.,Nakajima J.,他2名: "Cardiac xenotransplantation from pig to Japanese monkey with splenectomy,tacrolims,filtration plasmaphersis,and nafamstat mesilate." Transplantation Proceedings. 26. 1076-1077 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawauchi M.,Nakajima J.,他5名: "Which is the target vessel of lung rejection? A primate heart-lung transplantation study." Transplantation Proceedings. 26. 2338-2339 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawauchi M.,Nakajima J.,他3名: "Contribution of T cells in concordant xenoheart rejection." Transplantation Proceedings. 26. 1193-1194 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大岩 博、中島 淳、他3名: "FK506の血中及び臓器別濃度-ニホンザル肺移植における検討-" 移植. 29. 144-148 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Jun Nakajima: "Problems in xenograftd lung of the primates.(in Update clinical immunology,Molecular Science in Organ Transplantation)" Pabst Science Publishers,Lengerich,Germany (出版中), 20 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 中島 淳,他: "食道超音波内視鏡による肺門・縦隔リンパ節の部位同定に関する基礎的検討" 呼 吸. 13. 1043-1047 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 中島 淳,他: "結節性硬化症に合併した過誤腫性肺脈管筋腫症-特異な臨床修飾が加えられた1例-" 日本胸部疾患学会雑誌. 33. 80-84 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Kawauchi,J.Nakajima,et.al.: "Cardiac xenotransplantation from pig to Japanese monkey with splenectom Splenectom tacrolims,filtration plasmapheresis and nafamstat mesilate" Transplantation Proceedings. 26. 1076-1077 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Kawauchi,J.Nakajima,et.al.: "Which is the target vessel of lung rejection? A primate heart-lung transplantation study." Transplantation Proceedings. 26. 2338-2339 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大岩 博,中島 淳,他: "FK506の血中濃度及び臓器別濃度-ニホンザル肺移植における検討-" 移 植. 29. 144-148 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 吉竹 毅,中島 淳,他: "同種肺移植日本猿の移植肺および脾臓におけるTおよびBリンパ球の分布。" 日本呼吸器外科学会雑誌. 8. 129-134 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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