| Project/Area Number |
06454404
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | Tokyo Women's Medical College |
|---|
Principal Investigator |
NITTA Sumio Tokyo Women's Medical College, The Medical department, Proffesor, 医学部, 教授 (60006096)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OYAMA Kunihiro Tokyo Women's Medical College, The Medical department, assistant, 医学部, 助手 (10221699)
KAGURAOKA Haruhiko Tokyo Women's Medical College, The Medical department, Lecturer, 医学部, 講師 (10177339)
SONE Yasuyuki Tokyo Women's Medical College, The Medical department, Lecturer, 医学部, 講師 (70167477)
OHNUKI Takumasa Tokyo Women's Medical College, The Medical department, assistant Proffesor, 医学部, 助教授 (20194235)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥5,700,000 (Direct Cost: ¥5,700,000)
|
|---|
| Keywords | bronchial revascularization / bFGF / cytokines ploliferate blood vessels |
|---|
| Research Abstract |
We examined local effects of cytokines that proliferates blood vesseles such as basic fibroblast growth factor (bFGF) on early bronchial revascularization following bronchial anastomosis.
The left main bronchi of mongrel dogs (8-15 kg) were cut and sutured folloeing peripheral radical hilar stripping. The anastomotic sites were wrapped with apericardial fat tissue pedicle, including branches of the internal thoracic artery and vein. One ml of bFGF (Biomedical Technologies Inc.) dissolved in 1 ml of fibrin glue (final consentration of 1ng/ml, 10ng/ml, 20ng/ml, 40ng/ml, 100ng.ml, 1mug/ml, pH 7.1,7.2,7.3,7.4,7.5) was applied to the anastomotic sites in order to deliver the bFGF gradually and selectively).
At verious timepoints, changes in bronchial mucosal blood flow were measured using a laser Doppeler flowmeter and left internal thoracic artery (LITA) blood flow, using an implanted electromagnetic flowmeter. On the 7th postoperactive day, silicone rubber was injected into the left subclavian artery, and the revascularization pattern from branches of the LITA to the bronchus was examined histologically. Specimens were labeled as (-) when the neogeic vessels were only observed outside the bronchial wall, (1+) when observed inside the bronchial wall but not in the mucosa, and (2+) when clearly observed in the mucosa. Blood flow in the bronchial mucosa was measured as the left/right ratio. Futher more we examined bronchial revascuralization with SOFTEX.
As a results, most suitable consentration of bFGF was 40-100ng/ml and most suitable PH was 7.3-7.5 at the bronchial revascularization.
Other effective cytokines proliferate blood vessels was Platelet derived-endotherial cell growth factor (PD-ECGF) and Transforming growth factor-beta (TGF-beta).
We conclude that local application of cytokines proliferate blood vessels such as bFGF,PD-ECGF and TGF-beta is effective in stimulating early bronchial revascularization.
|
