• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Pathogenesis of Cerebral Vasospasm ; Evaluation of Ca^<2+> sensitivity of contractile proteins.

Research Project

Project/Area Number 06454410
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionUniversity of Tokyo

Principal Investigator

SASAKI Tomio  University of Tokyo, Dept.of Neurosurgery, Associate professor, 医学部(病), 助教授 (10134561)

Co-Investigator(Kenkyū-buntansha) YOSHIMOTO Yuhei  University of Tokyo, Dept.of Neurosurgery, Research Associate, 医学部(病), 助手 (50242061)
SAITO Nobuto  University of Tokyo, Dept.of Neurosurgery, Research Associate, 医学部(病), 助手 (60262002)
KIM Phyo  University of Tokyo, Dept.of Neurosurgery, Research Associate, 医学部(病), 助手 (90231290)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsCerebral Vasospasm / Calcium / Oxyhemoglobin / Subarachnoid Hemorrhage / オキシヘモグロビン / 平滑筋収縮 / カルシウムイオン
Research Abstract

The present study was undertaken to determine whether oxyhemoglobin (OxyHb) is responsible for the functional alterations in the cerebral arteries observed during chronic vasospasm after subarachnoid hemorrhage. Vascular strips of canine basilar arteries were kept in organ culture for 3 days with or without repetitive exposure to OxyHb (OxyHb-treated and control strips). Contractions elicited by high levels of potassium (80mM) and uridine 5'-triphosphate (310^<-4>M) were reduced in the OxyHb-treated group in a concentration-dependent manner. The relaxations evoked by nitric oxide and 8-bromo-cyclic guanosine monophosphate (8-bromo-cGMP) were not affected. Relaxations elicited by the calcium channel blocker, diltiazem, were attenuated in the OxyHb-treated rings, When the extracellular calcium concentration ([Ca^<++>] _e) was changed from a concentration in the external solution of 10^<-8> M to 10^<-3> M,myogenic tension developed. Myogenic tension, expressed as a percentage of the maximum contraction in each segment, was augmented in the OxyHb-treated group at [Ca^<++>] _e of 10^<-5> M and 10^<-4> M.There were no significant differences in passive compliance of the arterial wall between the two groups. These results demonstrated that prolonged exposure to OxyHb in vitro results in a decrease in contractile capacity and an increase in sensitivity to [Ca^<++>] _6, in agreement with previous findings in spastic arteries. By contrast, impairment of the 8-bromo-cGMP-mediated relaxation pathway and increased stiffnes of the arterial wall, which have been reported to occur in spastic arteries, were not induced by prolonged exposure to OxyHb in vitro.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Yoshimoto. Y, Kim P, Sasaki T, Kirino T,: "Functional changes in cultured strips of canine cerebral arteries after prelonged exposure to oxyhemoglobin" J Neurosurg. 83. 867-874 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kim P, Yoshimoto. Y, Iinc M, Sasaki T,: "Impaired calcium regulation of smooth muscle during chronic Vasospasm following Subarachnoid hemorrhage" J Cereb Blood Flow Metab. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshimoto Y,Kim P,Sasaki T,Kirino T,Takakura K.: "Functional changes in cultured strips of canine cerebral arteries atter prolonged exposure to oxyhemoglobin." J Neurosurg. 83. 867-874 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kim P,Yoshimoto Y,Lino M,Sasaki T,Kirino T,Nonomura Y.: "Impaired colcium regulation of smooth muscle during chronic vasospasmfollowing subarachnoid hemorrhage" J Cereb Blood Flow Metob. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yuhei Yoshimoto, Phyo Kim, Tomio Sasaki, Kintomo Takakura: Measurement of Intracellular Colcium and Force Development to Altered Extracellular Calcium Concentration in Spastic Cerebrol Arterier ofter Subarochnoid Hemorrage, In : Cerebrovascular Surgery, eds kintomo Takakura, Tomio Sasaki. University of Tokyo Rress, 116-119 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshimoto Y.,Kim P.,Sasaki T.,Kirimo.,Takakura K.: "Functional changes in cultured strips of canine cerebrol arteries after prolonged exposure to oxyhemoglobin." J Neurosurg. 83. 867-874 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kim P.,Yoshimoto Y.,Iino M.,Sasaki T.,Nonomura Y.: "Impaired calcium regulation of smooth muscle during chronic vasospasm following subarachnoid homorrhage" J Cereb Blood Flow Metab. (in press).

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi