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Molecular Biological Analysis of Invasion and Metastasis in Urological Cancer.

Research Project

Project/Area Number 06454458
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionThe University of Tokushima

Principal Investigator

KAGAWA Susumu  University of Tokushima, School of Medicine Chief Professor, 医学部, 教授 (40035738)

Co-Investigator(Kenkyū-buntansha) NARUO Seiichi  University of Tokushima, School of Medicine Assistant, 医学部, 助手 (20237621)
KANAYAMA Hiroomi  University of Tokushima, University Hospital, Assistant Professor School of Medi, 医学部・附属病院, 講師 (10214446)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1994: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsRENAL CELL CARCINOMA / BLADDER CANCER / GENE EXPRESSION / TUMOR INVASION / TUMOR METASTASIS / MATRIX METALLOPROTEINASE / TISSUE INIHIBITOR OF METALLOPROTEINASE / CD44 / nm23
Research Abstract

[Purpose] Invasions and metastasis-related factors in human bladder cancer and renal cell carcinoma were evaluated by various methods using molecular biological techniques.
[Materials and Methods] Gene expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) was determined in 22 bladder cancer tissues by Northern blot and slot blot analyzes. The localization of MMP-2 and TIMP-2 was evaluated immunohistochemically. To determine the correlation with the clinicopathological features, serum levels of interleukin-1b (IL-1b), interleukin-6 (IL-6) and tumor necrosis-a (TNF-a) were measured in 32 renal cancer patients and expression of CD44 variant forms was analyzed in 19 renal cancer tissues by the reverse transcriptasepolymerase chain reaction (RT-PCR) method.
[Results] The mRNA level of MMP-2, TIMP-2 and ratio of MMP-2/TIMP-2 expression were correlated with the pathological stage (p<0.05, p<0.05, p<0.05) and tumor progression (p<0.005, p=0.058, p<0.005). Immunohistochemically, MMP-2 was lacalized on the cell surface of invasive tumor cells and TIMP-2 was localized around the invasive tumor focus. In renal cancer, elevated serum IL-6 level and enhanced expression of alternative splice variant forms of CD44 involving the exon v10 were correlated with tumor progression and rapid growth of renal cell carcinoma.
[Conclusion] In bladder cancer, expression of MMP-2 and TIMP-2 was correlated with tumor invasion and progression. In renal cancer, serum levels of IL-6 and expression of CD44 variant forms sharing exon v10 was correlated with tumor progression and rapid growth.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 鳴尾精一・他: "膀胱癌におけるタイプIVコラゲナーゼとTissue inhibitor of metalloproteinases (TIMP)の遺伝子発現についての検討" 日本泌尿器科学会雑誌. 84. 841-850 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seiichi Naruo,et al.: "Serum levels of a tissue inhibitor of metalloproteinases-1 (TIMP-1) in bladder cancer patients" International Journal of Urology. 1. 228-230 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiro-omi Kanayama,et al.: "Analysis of nm23 gene expressions in human bladder and renal cancers" International Journal of Urology. 1. 324-331 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 浜尾巧・他: "腎細胞癌におけるインターロイキン1β(IL-1β)、インターロイキン6(IL-6)、腫瘍壊死因子-α(TNF-α)の血清値および遺伝子発現" 日本泌尿器科学会雑誌. 85. 563-570 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Kan,et al.: "High-level Expression of the CD44 Variant Exon v10 in Renal Cancer" Japanese Journal of Cancer Research. 86. 847-853 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 香川征: "泌尿器科腫瘍学における分子生物学" 日本泌尿器科学会雑誌. 85. 963-978 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seiichi Naruo, Hiro-omi Kanayama, Masashi Aki and Susumu Kagawa: ""Gene expressions of type IV collagenase and tissue inhibitor of metalloproteinases (TIMP) in human cancers. "" Japanese Journal of Urology. 84. 841-850 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seiichi Naruo, Hiro-omi Kanayama, Hiroshi Takigawa, Susumu Kagawa, Kyoko Yamashita and Taro Hayakawa: ""Serum levels of a tissue inhibitor of metalloproteinases-1 (TIMP-1) in bladder cancer patients. "" International Journal of Urology. 1. 228-230 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiro-omi Kanayama, Hiroshi Takigawa and Susumu kagawa: ""Analysis of nm23 gene expressions in human bladder and renal cancers. "" International Journal of Urology. 1. 324-331 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takumi Hamao, Hiro-omi Kanayama, Masaharu Kan, Hiroshi Takigawa and Susumu Kagawa: ""Serum levels and gene expressions of interluekin-1b (IL-1b), interluekin-6 (IL-6) and tumor recrosis factor-a (TNF-a) in human renal cell carcinomas. "" Japanese Journal of Urology. 85. 563-570 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Kan, Masashi Aki, Kinya Akiyama, Seiichi Naruo, Hiro-omi Kanayama and Susumu Kagawa: ""High-level Expression of the CD44 Variant Exon v10 in Renal Cancer. "" Japanese Journal of Cancer Research. 86. 847-853 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Kan, Atsuko Furukawa, Masashi Aki, Hiro-omi Kanayama and Susumu Kagawa: ""Expression of CD44 splice variants in bladder cancer. "" International Journal of Urology. 2. 295-301 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Susumu Kagawa: ""Molecular aspects of urological cancer. "" Japanese Journal of Urology. 85. 963-978 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiro-omi Kanayama, Keiji Kojima, Masashi Aki and Susumu Kagawa: ""Allelic loss of chromosome 17p in human urothelial cancer. "" Nishinihon Journal of Urology. 57. 732-736 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiro-omi Kanayama, Masaharu Kan, Masashi Aki and Susumu Kagawa: ""Expression of CD44 variant form in human renal cell carcinoma. "" Nippomrinsho. 53. 1710-1715 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiro-omi Kanayama, Masaharu Kan and Susumu Kagawa: ""Molecular biology of tumor invasion and metastasis in urological cancers. "" Nishinihon Journal of Urology. (in press). 58 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seiichi Naruo, et al.: "Serum levels of tissue inhibitor of metalloproteinases-1 (TIMP-1) in bladder cancer patients" International Journal of Urology. 1. 228-230 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiro-omi Kanayama, et al.: "Analysis of nm23 gene expressions in human bladder and renal cancers" International Journal of Urology. 1. 323-331 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 浜尾 巧・他: "腎細胞癌におけるインターロイキン1β(IL-1β)、インターロイキン6(IL-6)、腫瘍壊死因子-α(TNF-α)の血清値および遺伝子発現" 日本泌尿器科学会雑誌. 85. 563-570 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masaharu Kan, et al.: "High-level expression of the CD44 variant exon v10 in renal cancer" Japanese Journal of Cancer Research. 86. 847-853 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masaharu Kan, et al.: "Expression of CD44 splice variants in bladder cancer" International Journal of Urology. 2. 295-301 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 金山博臣・他: "腎細胞癌におけるCD44変異型の発現について" 日本臨床. 53. 1710-1715 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Seiichi Naruo: "Serum levels of tissue inhibitor of metalloproteinases-1(TIMP-1)in bladder cancer patients" International Journal of Urology. 1. 228-231 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hiro-omi Kanayama: "Analysis of nm23 expression in human bladder and renal cancers" International Journal of Urology. 1. 324-331 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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