| Project/Area Number |
06454463
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | AKITA UNIVERSITY |
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Principal Investigator |
TANAKA Toshinobu MD,PROFESSOR & CHIARMAN,DEPT.OF OB/GYN,AKITA UNIV.SCHOOL OF MEDICINE, 医学部, 教授 (40002216)
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Hirokazu MD,Assistant Professor, DEPT.OF OB/GYN,AKITA UNIV.SCHOOL OF MEDICINE, 医学部, 助手 (60272035)
曽我 賢次 秋田大学, 医学部, 講師 (70143055)
設楽 芳宏 秋田大学, 医学部, 講師 (40187357)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1994: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | Annexin V / Carcinogenesis / Platelet aggregation / Follicular maturation / Annexin V mRNA / Annexin V / calphobindin / 組織トロンボプラスチン / 血小板 |
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| Research Abstract |
(1)We examined the level of production of CPB I in uterine cancer cells. Immunohistochemical analysis, northern blot, and in situ hybridization showed that the production of CPB I was markedly suppressed at the level transcription in both cervical and endometrial carcinoma cells when compared to their normal counterparts. Decrease in production of CPB I may lead to dysregulated activation of protein kinase C and, accordingly, may be involved in a disorder of cell differentiation, proliferation, and carcinogenesis.(Ref 1)
(2)We demonstrated the localization and expression of annexin V in the reproductive organ, such as ovary, endometrium and fallopian tube. Signal of annexin V was strongly expressed in the follicular epitheliums of mature follicles and oocytes, which suggested an important role of annexin V during the process of follicular growth and oocyte maturation.(Ref 2)
(3)Existence of annexin V in platelet was firstly demonstrated by Western blot. Concentration of annexin V in platelet before the aggregation lowered significantry after the reaction. It is suggested that annexin V may play an important role of the regulation of platelet aggregation.(Ref 3)
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