| Project/Area Number |
06454464
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KUBO Takeshi Institute of Clinical Medicine, University of Tsukuba Professor, 臨床医学系, 教授 (20010267)
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| Co-Investigator(Kenkyū-buntansha) |
ARINAMI Tadao Institute of Basic Medical Sciences, University of Tsukuba Associate Professor, 基礎医学系, 助教授 (10212648)
HAMADA Hiromi Institute of Clinical Medicine, University of Tsukuba Assistant Professor, 臨床医学系, 講師 (60261799)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | Prenatal diagnosis / maternal blood / fetal nucleated cells |
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| Research Abstract |
The purpose of this study was to establish a new prenatal diagnostic method using fetal nucleated cells in maternal peripheral blood. The results of the study showed several new findings as follows ;
1. Fetal nucleated cells in maternal peripheral blood decrease in frequency with time after normal pregnancy and delivery. These cells disappear from maternal circulation 3 months after delivery in most, if not all, mothers.
2. Fetal nucleated cells in maternal peripheral blood become undetectable 3 months after delivery in most, if not all, cases of fetomaternal hemorrhage the same as in cases of normal pregnancy and delivery.
3. Fetal sex determination using fetal nucleated cells in maternal peripheral blood with FISH is possible. However, considering the time and labor required for the analysis of a much larger number of cells, the enrichment of fetal cells is needed before an analysis by this method.
4. The enrichment of fetal nucleated cells in maternal peripheral blood by fluorescence activated cell sortings is possible. Further improvement of this diagnostic method is needed to use in clinical medicine.
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