| Project/Area Number |
06454467
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Fukui Medical School |
|---|
Principal Investigator |
KOTSUJI Fumikazu Fukui Medical School, Department of OB/GYN,Associate Professor, 医学部, 助教授 (50153573)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1994: ¥3,800,000 (Direct Cost: ¥3,800,000)
|
|---|
| Keywords | Granulosa-Theca Cell Interaction / Ovary / Granulosa Cell Differentiation / Follicular Maturation / Theca Cell / Granulosa Cell / Ultrastructure / 機能分化 / Estradiol / Inhibin / Progesterone |
|---|
| Research Abstract |
We have developed a co-culture system in which granulosa and thecal cells were maintained on the opposite surface of a collagen membrane. Using this system, we have investigated the possible role of thecal cells in the conrol of the hormonal producing activity and the ultrastructure of granulosa cells during bovine ovarian follicular development. Thecal cells reduced estradiol, inhibin and progester one production by granulosa cells from early stage of follicular development, respectively, while augmented those by cells at late developmental stage. Morphological analysis of granulosa cells co-cultured with thecal cells shows a decreasein the clustering of lysosomes, shrinkage of endoplasmic reticulum and mitochondria of cells from small follicles but an enhancement of these cytodifferentiative features in cells from mature follicles when compared to the cells cultured alone.
These results demonstrate that the thecal cells secrete factor (s) which inhibits the differentiation of the immature granulosa cells while promoting that of the matured ones and suggest that the thecal cells play an important role in the control of granulosa cell differentiation during ovarian follicular development.
|
