| Project/Area Number |
06454469
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
KANAYAMA Naohiro Department of Obstet & Gyne Hamamatsu University School of Medicine, lecturer, 医学部附属病院, 講師 (70204550)
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| Co-Investigator(Kenkyū-buntansha) |
SUMIMOTO Kazuhiro Hamamatsu University, School of Medicine, assistant, 医学部, 助手 (30126817)
TERAO Toshihiko Hamamatsu University, School of Medicine, Professor, 医学部, 教授 (60022852)
能登 裕志 浜松医科大学, 医学部・附属病院, 講師 (80115500)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Interleukin-8 / Cervical maturation / IL-8 receptors / DHA-S / Collagenase / 早産 / 陣痛 / 切迫早産 / インターロイモン-8 / インターロイキン-8 / M-CSF / UTI |
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| Research Abstract |
IL-8 induced cervical ripening in non pregnant and pregnant rabbits. After treatment with IL-8 cervices become soft and dilatable. Water content and total glycosaminoglycan were increased, whereas collagen concentration was decreased. Neutrophil influx was observed after exogenous application of IL-8 to the cervices. Collagenase, elastase and gelatinase activities were increased. Similar results, but less marked, were found after treatment of the rabbit cervices with IL-1.
IL-8 receptors (type I and II) were expressed in the chorion and decidua before the initiation of labor, whereas amnion was negatively stained for IL-8 receptors. After initiation of labor pain, IL-8 receptors were strongly stained in amnion, chorion and decidua. IL-8 receptors were also expressed in myometrium.
Stratified squamous epithelium of portio vaginalis was strongly stained for IL-8 receptors (type I and II), whereas the columnar epithelium of the cervical glands was negatively stained for receptors. Cervical fibroblasts and smooth muscle fibers of the cervix were strongly stained for IL-8 receptors. The density of IL-8 receptors was increased after incubation with DHA-S,whereas no effect was found after incubation with E_2, DHA,or progesterone. DHA-S was also found to potentiate the ripening effect of IL-8 on the rabbit cervices.
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