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A Biochemical Study on the Role of Amnion and Decidua Vera Tissues in the Maintenance of Human Pregnancy

Research Project

Project/Area Number 06454472
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SAGAWA Norimasa  Kyoto University, Graduate School of Medicine, Department of Gynecology and Obstetrics, Associate Professor, 医学研究科, 助教授 (00162321)

Co-Investigator(Kenkyū-buntansha) ITOH Hiroaki  Kyoto University, Graduate School of Medicine, Department of Gynecology and Obst, 医学研究科, 助手 (70263085)
KONISHI Ikuo  Kyoto University, Graduate School of Medicine, Department of Gynecology and Obst, 医学研究科, 講師 (90192062)
小林 史典  京都大学, 医学部, 助手 (00243022)
伊原 由幸  京都大学, 医学部, 講師 (10223299)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥4,200,000 (Direct Cost: ¥4,200,000)
Keywordsamnion / amniotic fluid / brain natriuretic peptide / decidua vera / endothelin / maintenance of pregnancy / phospholipse D / prostaglandin
Research Abstract

To elucidate the role of amnion and decidua tissues in the maintenance of human pregnancy, the production of endothelin (ET) and brain natriuretic peptide (BNP), and the expression of receptors for these peptides in intrauterine tissues were investigated. In addition, we examined the physiological role of phospholipase D (PLD) in amnion.
The ET production by cultured amnion cells was stimulated by various cytokines and growth factors present in amniotic fluid. Ligand binding analysis and Northern blot analysis indicated that both ET-A receptor and ET-B receptor are present in chorion laeve, decidua vera, and myometrium, but not in the amnion tissue at term. However, amnion tissue in the second trimester expressed ET-A receptor.
The BNP production by cultured amnion cells was dose-dependently stimulated by TGF-beta, but was inhibited by cortisol or EGF.The TGF-beta augmented BNP production was blocked by the simultaneous treatment with cortisol or EGF.The receptor for BNP (particulate gua … More nylate cyclase A-type : GC-A) was identified in chorion, decidua, and myometrium by both cGMP generation assay and Northern blot analysis. However, GC-A was not detected in amnion tissues in the second trimester and at term. Thus, BNP secreted from amnion cells may not act on amnion tissue, but on the decidua and myometrium, and generate cGMP,the second messenger of BNP,and finally block uterine contraction during the second trimester. Such an inhibition of uterine contraction by BNP may be attenuated at term when the BNP secretion from amnion cells are suppressed by cortisol and EGF.
We previously reported the presence and biochemical characteristics of PLD in amnion rissue. The PLD activity in cultured amnion cells has been shown to be regulated by protein kinase C and Ca^<2+>. Phosphatidic acid (PA), the primary product of PLD,stimulated the PGE_2 secretion from cultured amnion cells. Moreover, PLD enhanced the ET secretion and inhibited the BNP secretion from cultured amnion cells. Thus, PLD plays important roles in the regulation of function of amnion cells and maintenance of human pregnancy.
In conclusion, various bioactive substances in the amniotic fluid of fetal origin act on amniotic membrane, modulate the production of ET,BNP,PA or PGE_2, and finally regulate the uterine contractility, v.i.z.the maintenance of pregnancy. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] H. Itoh, N. Sagawa, et al.: "Transforming growth factor-β stimulateds, and glucocorticoids and epidermal growth factor inhibit brain natriuretic peptide secretion from cultured human amnion cells." Journal of Clinical Endocrinology and Metabolism. 79(1). 176-182 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H. Itoh, N. Sagawa, et al.: "Expression of biologically active receptors for natriuretic peptides in the human uterus during pregnancy." Biochemical and Biophysical Research Communications. 203(1). 602-607 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N. Sagawa, et al.: "Current topic: The role of amniotic endothelin in human pregnancy." Placenta. 15. 565-575 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N. Sagawa, et al.: "Secretion of endothelin by avascular amnion tissue: Possible roles in human pregnancy." Endocrine J.41(Suppl). S117-S125 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kumiko Inamori, Norimasa Sagawa, et al.: "Activation of phospholipase D in cultured human amnion cells." Reproduction, Fertility and Development. 7(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaki Hasegawa, Norimasa Sagawa, et al.: "Endothelin 1-like immunoreactivity and endothelin receptors in the human placenta from normotensive and hypertensive pregnancies." Journal of Perinatal Medicine. 23(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N. Sagawa, et al.: "Biochemical characterristics on phospholipase D in human amniotic membrane. In:Current Progress in Perinatal Medicine, (eds)E. V. Cosmi et al." The Parthenon Publishing Group, New York and London, 5 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐川典正、森 崇英: "疾病と栄養 14.妊娠と栄養「ベッドサイドの栄養学」糸川嘉則、松倉 茂、清野 裕、編著" 南山堂, 12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Itoh, N.Sagawa, et al.: "Transforming growth factor-beta stimulates, and glucocorticoids and epidermal growth factor inhibit brain natriuretic peptide secretion from cultured human amnion cells" Journal of Clinical Endocrinology and Metabolism. 79(1). 176-182 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Itoh, N.Sagawa, et al.: "Expression of biologically active receptors for natriuretic peptides in the human uterus during pregnancy" Biochemical and Biophysical Research Communications. 203(1). 602-607 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Sagawa, et al.: "Current topic : The role of amniotic endothelin in human pregnancy" Placenta. 15. 565-575 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Sagawa, et al.: "Secretion of endothelin by avascular amnion tissue : Possible roles in human pregnancy" Endocrine J.41(Suppl). S117-S125 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kumiko Inamori, Norimasa Sagawa, et al.: "Activation of phospholipase D in cultured human amnion cells" Reproduction, Fertility and Development. Vol.7. (in press) (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaaki Hasegawa, Norimasa Sagawa, et al.: "Endothelin 1-like immunoreactivity and endothelin receptors in the human placenta from normotensive and hypertensive pregnancies" Journal of Perinatal Medicine. Vol.23. (in press) (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Sagawa, et al.: "Biochemical characterristics on phospholipase D in human amniotic membrane" In : Current Progress in Perinatal Medicine, (eds) E.V.Cosmi et al., The Parthenon Publishing Group, New York and London. 401-406 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Sagawa, et al.: "Pregnancy and nutrition" In : Diseases and Nutrition, (eds) Y.Itokawa et al.361-372 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuminori Kobayashi: "Biochemical and topological analysis of adult T-cell luekemia-derivedfactor, homologous to thioredoxin, in the pregnant human uterus; Posible role of strong expression in decidua and trophoblasts." Molecular Human Reproduction. 10(6). 1603-1608 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kumiko Inamori: "Activation of phospholipase D in cultured human amnion cells" Reproduction, Fertility and Development. 7(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroaki Itoh: "Umbilical venous guanocsine3', 5'-cyclic phosphate(cGMP) concentration increases in asphyxyated newborns." Reproduction,Fertility and Development. 7(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masaaki Hasegawa: "Endothelin receptors in the human amnion, chorion laeve, decidua vera and placenta." Reproduction, Fertility and Development. 7(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masaaki Hasegawa: "Endothelin 1-like immunoreactivity and endothelin receptors in the human placenta from normotensive and hypertensive pregnancies." Journal of Perinatal Medicine. 23(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsunekazu Matsumoto: "The type 2 angiotensin II receptor is expressed in human myometrium and uterine leiomyoma, and is down-regulated during pregnancy" Jounal of Clinical Endocrinology and Metabolism. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐川典正: "疾病と栄養 14.妊娠と栄養 「ベッドサイドの栄養学」糸川嘉則、松倉 茂、清野 裕、編著" 南山堂, 12 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Itoh,et al: "Transforming growth factor-β stimulates,and dlucocorticoids and epidermal growth factor inhibit brain natriuretic peptide secretion from cultured human amnion cells." Journal of Clinical Endocrinology and Metabolism. 79. 176-182 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] F.Kobayashi et al.: "Platelet-activating factor-acetylhydrolase activity in maternal and umbilical venous plasma obtained from normotensive and hypertensive pregnancies." Obstetrics and Gynecology. 84. 360-364 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Itoh,et al: "Expression of biologcally active receptors for natriuretic peptides in the human uterus during pregnancy." Biocehmical and Biophysical Research Communications. 203. 602-607 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Sagawa et al: "Current topic:The role of amniotic endothelin in human pregnancy." Placenta. 15. 565-575 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Okagaki,et al: "Clinical application of pulsatility index of flow volume to detect the hemodynamic changes in IUGR fetus." J.Perinat.Med.22. 243-251 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Sagawa,et al: "Secretion of endothelin by avascular amnion tissue:Possible roles in human pregnancy." Endocrine J.41. S117-S125 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Sagawa,et al: "Biochemical characterristics on phospholipase D in human amniotic membrane. In:Current Progress in perinatal Medicine pp401-406" The Parthenon Publishing Group, 902 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐川典正: "「E.呼吸器疾患」In:産婦人科学書 2 周産期医学 pp300-307" 金原出版, 817 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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