| Project/Area Number |
06454477
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yokohama City University |
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Principal Investigator |
MINAGUTI Hirosi INSTITUTION,DEPARTMENT,TITLE OF POSITION Professor, 医学部, 教授 (20010176)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASI Tuneo Yokohama City Univ. OB / GY,Assist.Prof., 医学部, 講師 (60179497)
TAGA Mitiyosi INSTITUTION,DEPARTMENT,TITLE OF POSITION Associate Prof., 医学部, 助教授 (00107682)
茂田 博行 横浜市立大学, 医学部, 講師 (20275051)
池田 万里郎 横浜市立大学, 医学部, 助手 (50254173)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | preimplantation / embryo / growth factor / Activin / TGF-beta / RT-PCR |
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| Research Abstract |
Purpose : Our purpose was to clarify the involvement of transforming growth factor-beta (TGF-beta) family in the regulation of preimplantation embryo development.
Methods : The effects of activin-A and TGF-beta on the rates of morula and blastocyst formations as well as on the cleavage velocity of a mouse two-cell embryo in vitro were analyzed. The gene expressions of these two growth factors in various developmental stages were also studied using RT-PCR.
Results : Activin-A at a concentration of 0.2 ng / ml significantly stimulated not only the rate of morula formation but also the velocity of embryo cleavage, whereas no significant effect was found with TGF-beta. RT-PCR revealed that activin-A subunit mRNA,but not TGF-beta mRNA,was detected in preimplantation mouse embryo at any developmental stage.
Conclusions : Activia-A plays an important role in the regulation of preimplantation mouse embryo development in an autocrine fashion.
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