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Gene expression analysis of human corneal and conjunctival epithelium by random cDNA sequencing

Research Project

Project/Area Number 06454500
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

KINOSHITA Shigeru  Medicine, Ophthalmology, Kyoto Prefectural University of Medicine, Professor, 医学部, 教授 (30116024)

Co-Investigator(Kenkyū-buntansha) OKUBO Kousaku  Molecualr and Cellular Biology, Osaka University, Assistant Professor, 細胞生体工学センター, 助教授 (40233069)
NISHIDA Kohji  Medicine, Ophthalmology, Kyoto Prefectural University of Medicine, Instructor, 医学部, 助手 (40244610)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥4,500,000 (Direct Cost: ¥4,500,000)
Keywordshuman / corneal epithelium / cDNA library / random sequence / gene expression / known gene / unknown gene / in vivo / cDNAライブラリー
Research Abstract

We studied the composition of mRNA in human corneal epithelium by the random cDNA sequencing. The expression profile of active genes in human corneal epithelium showed that the most adundant transcripts represented those of genes for apolipoprotein J (apoJ), calcyclin, alpha-enolase, human adipose mRNA and a novel gene.
The expression of both mRNA and protein of the most abundant trascripts, apoJ was investigated in corneal and conjunctival epithelium, as well as other mucosal epithelia (vagina, esophagus, stomach, small intestine, and colon) and epidermis. ApoJ mRNA transcripts was detected in corneal, conjunctival, vaginal and esophageal epithelium, but not in epidermal keratinocytes. ApoJ mRNA localized in all layrs of corneal epithelium, prominently in basal layr. ApoJ protein localized in the apical cell layrs of corneal and conjunctival epithelium, as well as various mucosal epithelia including vagina, esophagus stomach, small intestine and colon, but not in epidermis. These results indicated that apoJ may play a physiologically important role at the tear-ocular surface interface.
We cloned the full lenght cDNA of the novel gene. The cDNA contained ORF highly homologous to mouse keratin 12, indicating that this gene is human cornea-specific keratin K12.
Regarding the cDNA library construction of conjunctival epithelial cells, we are studying the sample collection method without contamination by blood cells or subepithelial cells.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Nishida K.et al.: "A gene expression prcfile of human corneal epithelium and the isolation of human Keratin 12 _cDNA." Investigative Ophthalmology & Visual Science submillin.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishida K.et al.: "Apolipoprotein J expression in human ocular surface epithelium." Investigative Ophtalmology & Visual Science,submitted.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishide K.et al.: "SP-40,40 erpression in human covneal epithelium" Ocular Cell & Molecular Biology Symposium II (San Diego. USA) ,Abstrhct. 24 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kokawa N.et al.: "High total TGF-β2 levels in normal human tears." Current Eyc Research,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sotozono C,et al.: "Keratinocyte growth factor accelerate corneal epithelial wound healing in vivo" Investigative. Ophthalmology & Visual Science. 36. 1524-1529 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishida K.et al.: "Transforming grath factor-β1,β2,-β3_mRNA expression in human corhea" Current Eye Research. 14. 235-241 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 木下茂(編集): "角膜疾患の細胞生物学" メジカルビュー, 172 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishida K,Adachi W,Shimizu-Matsumoto A,Kinoshita S,Matsubara K,okubo K: "A gene expression profile of human corneal epithelium and the isolation of human keratin 12 cDNA" Investigative Ophthalmology & Visual Science. (submitted). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishida K,Kawasaki S,Adachi W,Kinoshita S: "Apolipoprotein J expression in human ocular surface epithelium" Investigative Ophthalmology & Visual Science. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishida K,Simizu A,Kinoshita S,Matubara K,Okubo K: "SP-40,40 expression in human corneal epithelium" Ocular Cell & Molecular Biology Symposium II (San Diego, USA). Abstract, 24.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kokawa N,Sotozono C,Nishida K,Kinoshita S: "High total TGF-beta2 level in normal human tears" Current Eye Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sotozono C,Inatomi T,Nakamura M,Kinoshita S: "Keratinocyte growth factor accelerate corneal epithelial wound healing in vivo" Investigative Ophthalmology & Visual Science. 36. 1524-1529 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishida K,Sotozono C,Adachi W,Yamamoto S,Yokoi N,Kinoshita S: "Transforming growth factor -beta1, -beta2, -beta3 mRNA expression in human cornea" Current Eye Research. 14. 235-241 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishida K: "A gene expression profile of human corneal epthelium and the isolation of human keratin 12 cDNA." Investigative Ophthalmology & Visual Science. submitted.

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishida K: "Apolipoprotein J expression in human ocular surface epithelium." Investigative Ophthalmology & Visual Science. submitted.

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishida K: "SP-40,40 expression in human corneal epithelium." Ocular Cell & Molecular Biology Symposium II(San Diego,USA),Abstract. 24 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kokawa N: "High total TGF-β2 levels in normal human tears." Current Eye Research. in press.

    • Related Report
      1995 Annual Research Report
  • [Publications] Sotozono C.: "Keratinocyte growth factor accelerate corneal epithelial wound healing in vivo." Investigative Ophthalmology & Visual Science. 36. 1524-1529 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishida K: "Transforming growth factor-β1,-β2,-β3mRNA expression in human cornea." Current Eye Research. 14. 235-241 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 木下茂(編集): "角膜疾患の細胞生物学" メジカルビュー, (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 西田幸二,木下茂他: "Immvnohistochemical localization of GF-β_1,β_2,β_3 latency-dssociated peptide in human corned" Investigative Opnthalniology Visual Science. 35. 3289-3294 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 外園千恵,木下茂他: "Expression of keratinocyte gromth factor in RNA in ex vivc and in vivo specimens of human carned and conjwectivd" Japanese Jownal of ophthalmology. 38. 353-359 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 外園千恵、木下茂他: "Paracrine roke of keratinocyte growth tactor in rabbit cornedl epithelial all gronth" Experimental Eye Research. 59. 385-392 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 西田幸二,木下茂他: "Transforming growth factor-β_1,β_2,β_3mRNA expression in human comxd" Carrent Eye Research. (印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] 木下 茂(編集): "角膜疾患の細胞生物学" メジカルビュー社, 172 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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