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Dynamic changes of basement membrane molecules and degradation enzymes in stroma remodelled by proliferation and in asion of salivary gland carcinoma cells.

Research Project

Project/Area Number 06454508
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionNiigata University

Principal Investigator

SAKU Takashi  Niigata University School of Dentistry ; Professor, 歯学部, 教授 (40145264)

Co-Investigator(Kenkyū-buntansha) ODA Kimimitsu  Niigata University School of Dentistry ; Professor, 歯学部, 教授 (10122681)
KIMURA Shin  Niigata University School of Dentistry ; Assistant Professor, 歯学部, 助手 (80251825)
CHENG Jun  Niigata University School of Dentistry ; Assistant Professor, 歯学部, 助手 (40207460)
FUKUSHIMA Masahiro  Niigata University School of Dentistry ; Associate Professor, 歯学部, 助教授 (00018631)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1995: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1994: ¥4,700,000 (Direct Cost: ¥4,700,000)
KeywordsAdenoid cystic carcinoma / ACC2 / ACC3 / Basement membrane / Confoal laser microscopy / Heparan sulfate proteoglycan / Heparanase / Collagenase
Research Abstract

The characteristic cribriform histology of adenoid cystic carcinoma (ACC) arising in the human salivary gland is caused by retention of basement membrane molecules (BMMs) in the pseudocystic space. However, it is unknown whether the characteristic stroma of ACC is produced by the parenchymal cells or by stromal cells.
In this study, we determined biosynthesis of BMMs in the two human ACC cell systems, ACC2 and ACC3 immuno-histochemically and biochemically. Immunofluorescence for fibronectin and the four BMMs : type IV collagen, laminin, entactin and heparan sulfate proteoglycan (HSPG) first appeared diffusely in the cytoplasm, and they changed into aggregation of granules in the perinuclear area. With formation of colonies, these signals were present in the extracellular space and co-localized with heparanase and other enzymes. After the cells formed a confluent monolayr, extracellular signals started to decrease in the reverse proportion of reappearance of intracellular ones. When cultured in collagen gel, the cells formed spherical colonies with vacuolar structures containing BMMs. Such processes of biosynthesis and degradation of BMMs was confirmed by metabolic labeling, immunoprecipitation and fluorography for HSPG.
The results indicated that the parenchymal cells of ACC synthesize the BMMs and fibronectin, secrete them into the extracellular milieu, and remodel the extracellular deposits. It is hence suggested that the characteristic stromal pseudocysts of ACC is resulted from their own secretion and enzymatic degradation of the BMMs and fibronectin by the tumor cells.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Yoshizawa M.et al.: "Histopathological study on lymphatic invasion in squamous cell carcinoma (O-1N) with high potential of lymph node metastasis." Clinical & Experimental Metastasis. 12. 347-56 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cheng J.et al.: "Heparan sulfate proteoglycan turnover in adenoid cystic carcinoma of salivary gland" Proceedings of 7th Meeting of IAOP. 27 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Oda K.et al.: "Conversion of secretory proteins into membrane proteines by fusing with a glycosylphosphatidylinositol anchor signal of alkaline phosphatase" Biochemical Journal. 310. 577-83 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi Y,et al: "Loss of basement membranes in the invading fron of 0-1N,hamster squamous cell carcinoma with highly lymph node metastatic potential : an immunohistochemical study for laminin and type IV collagen." Patholog International. 45. 327-34 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cheng J,et al: "Biosynthesis of basement membrane molecules by salivary adenoid cystic carcinoma cells : an immunofluorescence and confocal microscopic study" Virchows Archiv. 426. 577-86 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura S.et al: "Biosynthesis and secretory pathway of basement membrane moloecules in salivary adenoid cystic carcinoma cells." Abstracts of 4th International Congress on Oral Cancer. 74 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshizawa M,et al.: "Histopathological study on lymphatic invasion in squamous cell carcinoma(O-1N)with high potential of lymph node metastasis." Clinical & Experimental Metastasis. 12. 347-56 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cheng J,et al.: "Heparan sulfate proteoglycan turnover in adenoid cystic carcinoma of salivary gland" Proceedings of 7th Meeting of IAOP. 27 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Oda K,et al.: "Conversion of secretory proteins into membrane proteines by fusing with a glycosylphosphatidylinositol anchor signal of alkaline phosphatase" Biochemical Journal. 310. 577-83 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi Y,et al.: "Loss of basement membranes in the invading fron of O-1N,hamster squamous cell carcinoma with highly lymph node metastatic potential : an immunohistochemical study for laminin and type IV collagen." Pathology International. 45. 327-34 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cheng J,et al.: "Biosynthesis of basement membrane molecules by salivary adenoid cystic carcinoma cells : an immunofluorescence and confocal microscopic study" Virchows Archiv. 426. 577-86 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura S,et al.: "Biosynthesis and secretory pathway of basement membrane molecules in salivary adenoid cystic carcinoma cells." Abstracts of 4th International Congress on Oral Cancer. 74 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshizawa M,et al.: "Histopathological study on lymphatic invasion in squamous cell carcinoma (O-IN) with high potential of lymph node metastasis." Clinical & Experimental Metastasis. 12. 347-56 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Cheng J,et al.: "Heparan sulfate proteoglycan turnover in adenoid cystic carcinoma of salivary gland" Proceedings of 7th Meeting of IAOP. 27- (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oda K,et al.: "Conversion of secretory prpteins into membrane proteines by fusing with a glycosylphosphatidylinositol anchor signal of alkaline phosphatase" Biochemical Journal. 310. 577-83 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kobayashi Y,et al.: "Loss of basement membranes in the invading fron of O-IN,hamster squamous cell carcinoma with highly lymph node metastatic potential: an immunohistochemical study for laminin and type IV collagen." Pathology International. 45. 327-34 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Cheng J,et al.: "Biosynthesis of basement membrane molecules by salivary adenoid cystic carcinoma cells: an immunofluorescence and confocal microscopic study" Virchows Archiv. 426. 577-86 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kimura S,et al.: "Biosynthesis and secretory pathway of basement membrane molecules in salivary adenoid cystic carcinoma cells." Abstracts of 4th International Congress on Oral Cancer. 74- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Cheng Jun et al: "Biosynthesis of basement membrane molecules by salivary adenoid cystic carcinoma cells:an immunofluorescence and confocal microscopic study" Virchows Archiv. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Cheng Jun & Saku Takashi: "Heparan sulfate proteoglycan turnoverin adenoid cystic carcinoma of salivary gland" 7th Meeting of IAOP,Abstract. 27 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 程 くん 他: "唾液腺腺様嚢胞癌細胞-線維芽細胞混合培養における細胞外基質の生合成" 日本病理学会会誌. 83. 172 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 木村 信 他: "腺様嚢胞癌由来ACC3細胞におけるヘパラン硫酸プロテオグリカン・コア蛋白質の検出" 日本病理学会会誌. 83. 172 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 木村 信 他: "唾液腺癌細胞におけるヘパラン硫酸プロテオグリカンの合成と分泌" 生化学. 66. 656 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 棟方 隆一他: "腺様嚢胞癌細胞ACC3のコラゲンゲル包埋培養における細胞外基質の生合成" 新潟歯学会誌. 24. 89 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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