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Gene Expression and Development of Odontogenic tumors

Research Project

Project/Area Number 06454511
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionOkayama University

Principal Investigator

NAGAI Noriyuki  Okayama University, School of Dentistry, Professor, 歯学部, 教授 (90085770)

Co-Investigator(Kenkyū-buntansha) KAWAHARA Kenji  Okayama University, School of Dentistry, Associate Professor, 歯学部, 助教授 (50076113)
AKAGI Takumi  Okayama University, School of Dentistry, Teaching Assistant, 歯学部, 教務員 (50192878)
MURATA Masaru  Okayama University, School of Dentistry, Assistant, 歯学部, 助手 (00260662)
INOUE Masahisa  Okayama University, School of Dentistry, Assistant, 歯学部, 助手 (20223274)
NAGATSUKA Hitoshi  Okayama University, School of Dentistry, Associate Professor, 歯学部, 助教授 (70237535)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1995: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1994: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsOdontogenic tumors / Amelogenin / Ameloblastoma / Adenoid odontogenic tumors / mRNA / Digoxigenin Labeling / Ameloblast / Columnar cells / Ameloblastoma / エナメル上皮腫 / ras蛋白 / c-erbB-2蛋白 / PCNA / p53蛋白 / 悪性エナメル上皮腫 / c-myc蛋白 / 免疫組織化学
Research Abstract

The classification of odontogenic tumors is related to the differentiation degree of tumor cells. The localization of amelogenin protein and the expression of amelogenin mRNA are among the indexes of odontogenic tumor cell defferentiation. In our study the probes of amelogenin protein and amelogenin mRNA were made and used to observe their distribution in odontogenic tumors.
Materials and methods : 1.Normal control : Wistar strain rat embryoes at 20 days after gestation were used for investigation of amelogenin protein and mRNA distribution in tooth germ.The specimens were fixed by 4% paraformaldehyde perfusion. 2.Making of probes : Total RNA was extracted from human plexiform type ameloblastoma. Primers (sense : 5-TCGGTCCTGCCTCAT-CACCATCC-3 ; antisense : 5-CCGCTTGGTCTTGTCTGTTG-3) were used for amplification using PCR method. The amplified cDNA was loaded on Vector (Bluescript) and labeled with Dig RNA Labeling Kit (Boehringer Mannheim) to make Digoxigenin labeled single strand RNA prob … More e by in vitro transcription. 3.Hybridization : The probe was hybridizad with the specimens at 50^0C for 16 hours. Antigen-antibody reaction was performed using Nucleic acid Detection Kit (Boehringer Mannheim) as to evaluate the hybridization under light microscope. In addition, polyclonal anti-amelogenin antibody was used to localize the distribution of amelogenin protein in odontogenic tumors.
Results and discussion : Both amelogenin protein and mRNA were positive in rat and mouse incisors but negative in undifferentiated enamel epithelia. Amelogenin mRNA was detected frequently in the cells of the central region of the follicles and usually positive in the cytoplasm of columnar cells near to basement membrane while amelogenin protein was occasionally detected only in a few cases of ameloblastomas. These findins suggest that amelogenin mRNA is generally present in ameloblastomas. Amelogenin mRNA was commonly detected in adenoid ondontogenic tumor cells. In addition the localization of amelogenin protein was also observed in adenoid odontogenic tumors. These results proved at molecular level that adenoid odontogenic tumor is a well differentiated tumor. Apart from the above-mentioned investigations immunohistochemistry was also adopted to evaluate cell proliferation, oncogene product and antioncogene product in ameloblastomas. These immunohistochemical studies indicated that immunodetection of PCNA,c-myc protein, ras protein and p53 protein is helpful in evaluating the malignance of ameloblastomas. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (69 results)

All Other

All Publications (69 results)

  • [Publications] Liliana Missana: "Comparative histological studies of bone and cartilage formations induced by various BMP-carrier composites" 歯科基礎医学会. 36. 9-19 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 長塚 仁: "BMP誘導異所性硬組織界面の骨芽細胞分化と骨基質蛋白遺伝子の発現" J. Hard Tissue Biol.3. 7-12 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 寒河江登志朗: "合成ヒドロキシアパタイトの結晶学的性質と細胞毒性試験" 日大口腔科学. 20. 40-48 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 井上正久: "右口底部唾液腺腫瘍" 病院病理. 11. 5-5 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N. Nagai: "Immunohistochemical demonstration of tenaiscin and fibronectin in odontogenic tumors and human tooth germs" J. Europian Pathology (B) Oral Oncology. 30. 191-195 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 榎本昭二: "歯科インプラントの現状に関する調査研究" 日本歯科医学会雑誌. 13. 53-75 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 日名雅彦: "歯原性腫瘍のエナメル基質蛋白,IVコラーゲン,ファイブロネクチン局在の免疫組織科学的研究" 岡山歯学会雑誌. 13. 57-66 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 山近英樹: "歯原性腫瘍および歯胚におけるテネイシン,IVコラーゲン,ファイブロネクチン局在の免疫組織科学的検索" 岡山歯学会雑誌. 13. 49-56 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 長井教之: "生体代替材料の組織界面の成立と異所性骨誘導蛋白BMPの登場" 東京都歯科医師会誌. 42. 533-543 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Inoue: "Histopathological Studies of Heterotopic Bone Formation Induced by BMP-membrane Carrier Composites" J. Jpn. Soc. Oral Implant.7. 243-248 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 村田 勝: "牛骨誘導蛋白(BMP)による異所性骨誘導の病理組織化学的研究" J. Jpn. Soc. Oral Implant.7. 230-236 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H. Nagatsuka: "Histopathological studies of Heterotopic bone formation induced by BMP-collagen Bead carriers" J. Jpn. Soc. Oral Implant.7. 237-242 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 林 勝彦: "エナメル上皮腫における細胞増殖能と癌遺伝子産物,癌抑制遺伝子産物発現の相関" 岡山歯学会誌. 14. 15-26 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N. Nagai: "Gene Expression of Bone Matrix Protein mRNA during BMP Induced Chondrogenesis and Osteogenesis by in situ Hybridization" J. Hard Tissue Biology. 4. 15-23 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hong-In Shin: "The Effect of Enzymatic Treatment on Immunohistochemical Stainning of Collagens in Madibular Condyle of Rats" J. Hard Tissue Biology. 4. 8-14 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 米本嘉憲: "口腔扁平上皮癌におけるp53癌抑制遺伝子産物と増殖細胞核抗原(PCNA)の免疫組織化学的検討" 日口外誌. 41. 46-50 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Sakae: "Crystallographic Properties and Cell Affinity Study of Synthetic α - tricalcium Phosphate, Tetracalcium Phosphate and Fluorapatie" J. Hard Tissue Biology. 3. 73-78 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 井上正久: "顎骨セメント質-骨形成性繊維腫と化骨繊維腫の発生組織に関する一考察" J. Hard Tissue Biology. 4. 9-14 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 永井教之: "アパタイト-骨界面の初期石灰化とBMPによる異所性骨誘導" J. Hard Tissue Biology. 4. 79-90 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 林 勝彦: "エナメル上皮腫,悪性エナメル上皮腫の増殖細胞核抗原(PCNA)による免疫組織科学的検討" 口腔科学会雑誌. 44. 7-13 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Kuboki: "Two Distinctive BMP-carriers Induce Zonal Chondrogenesis and Membranous Ossification, Respectively ; Genometorical Factors of Materials for Cell Differention" Connective Tissue Research. 31. 1-8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Murata: "Histopathological Studies of Heterotopic Bone Formation Induced by BMP Carrier Composites" J. Hard Tissue Biology. 3. 67-72 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 長塚 仁: "硬組織研究の基礎技術 -検査材料の免疫組織科学-" 日本硬組織技術学会編 学際企画, 120 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Liliana Missana: "Coparative histological studies of bone and cartilage formations induced by various BMP-carrier composites" Jpn.J.Oral Biol.Vol.36(1). 9-19 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitoshi Nagatsuka: "Histopathological Studies of Heterotopic Bone Formations Induced by BMP-HAP Carrier Composites" J.Hard Tissue Biol.Vol.3(1). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshiro Sakae: "Crystallographic Properties and Cell Affinity Study of a Series of Synthetic Hydroxyapatites" Nihon Univ.J.Oral Sci.Vol.20(1). 40-48 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masahisa Inoue: "Salivary Gland tumor at light mouse floor" J.Jpn.Assoc.Pathol.11(2)5. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Nagai: "Immnohistochemical demonstration of tenaiscin and fibronectin in odontogenic tumors and human tooth germs" J.Europian Pathology (B) Oral Oncology. vol30(3). 191-195 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shoji Enomoto: "Investigation of the Present Status of Dental Implant in Japan" J.Jpn.Dent.Assoc.Vol.13. 53-75 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] masahiko Hino: "Immunohistochemical Demonstration of Enamel Matrix Proteins, Type IV collagen and Fibronectin in Odontogenic Tumors" J.Okayama Dent.Soc.Vol.13(1). 57-66 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Eiki Yamachika: "Immunohistochemical Localization of Tenascin, Fibronectin and Type IV collagen in Odontogenic Tumors and Tooth Germ" J.Okayama Dent.Soc.Vol.13(1). 49-56 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Noriyuki Nagai: "The bone-implant iterface of bioinert materials, and Bone morphogenic proteins" J.Tokyo Soc.Dentisry. Vol.42(9). 533-543 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Inoue: "Histopathological Studies of Heterotopic Bone Formation Induced by BMP-membrane Carrier Composites" J.Jpn.Soc.Oral Implant.Vol.7(2). 243-248 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaru Murata: "Histopathological Studies of Heterotopic Bone Formations Induced by BMP-HAP Carrier Composites" J.Jpn.Soc.Oral Implant.Vol.7(2). 230-236 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Nagatsuka: "Histopathological studies of Heterotopic bone formation induced by BMP-collagen Bead carriers" J.Jpn.Soc.Oral Implant.Vol.7(2). 237-242 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitoshi Nagatsuka: "Basic Technique of hard tissue reserch-Technique of immunohistochemistry for surgical materisals-" ed.The Japanese Society of Hard Tissue Research & Technology Gakusaikikaku. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Hayashi: "Oncogene and tumor suppressor gene products in ameloblastoma : Correlation with cell kinetics" J.Okayama Dent.Soc.Vol.14(1). 15-26 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Nagai: "Gene Expression of Bone Matrix Protein mRNA during BMP Induced Chondrogenesis and Osteogenesis by in situ Hybridization" J.Hard Tissue Biol.Vol.4(1). 15-23 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hong-In Shin: "The Effect of Enzymatic Treatment on Immunohistochemical Stainning of Collagens in Mandibular Condyle of Rats" J.Hard Tissue Biol.Vol.4(1). 8-14 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yonemoto: "Immunohistochemical study of p53 tumor suppressor gene product and proliferating cell nuclear antigen in oral squamous cell carcinoma" Jpn.J.Oral Maxillofac.Surg.41(9). 46-50 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.SAKAE: "Crystallographic Properties and Cell Affinity Study of Synthetic alpha-tricalcium Phosphate, Tetracalcium Phosphate and Fluorapatite" J.Hard Tissue Biol.vol3(2). 73-78 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Inoue: "Histopathological study of cemento-ossifying fibroma and ossifying fibroma in jaw bone" J.Hard Tissue Biol.Vol.4(3). 9-14 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Nagai: "Histopathological Studies of Heterotopic Bone Formation Induced by BMP and early calcification on surface of apatite and bone" J.Hard Tissue Biol.Vol.4(3). 79-90 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Hayashi: "Immunohistochemical expressions of PCNA in ameloblastoma and malignant ameloblastoma" J.Jpn.Stomatrol.Soc.Vol.44(1). 7-13 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.KUBOKI: "Two Distinctive BMP-carriers Induce Zonal Chondorogenesis and Membranous Ossification, Respectively ; Genometorical Factors of Materiales for Cell Differention" Connective Tissue Research.vol.31(3). 1-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.MURATA: "Histopathological Studies of Heterotopic Bone Formation Induced by BMP-membrane Carrier Composites" J.Hard Tissue Biol.vol3(2). 67-72 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 林 勝彦: "エナメス上皮腫,悪性エナメル上皮腫の増殖細胞核抗原(PCNA)による免疫組織化学的検討" 日本口腔科学会誌. 44. 7-13 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Noriyuki Nagai: "Gene Expression of Bone Matrix Protein mRNA during BMP Induced chondrogenesis and Osteogenesis by in situ Hybridization" Journal of Hard Tissue Biology. 4. 15-23 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 林 勝彦: "エナメス上皮腫における細胞増殖能と癌遺伝子産物,癌抑制遺伝子産物発現の相関" 岡山歯学会誌. 14. 15-26 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 米本 嘉憲: "口腔扁平上皮癌における癌抑制遺伝子産物と増殖細胞核抗原(PCNA)の免疫組織化学的検討" 日本口腔外科学会誌. 41. 746-750 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 井上 正久: "歯原性腫瘍のエナメル基質蛋白,IVコラーゲン,ファイブロネクチン局在の免疫組織化学的研究" Journal of Hard Tissue Biology. 4. 9-14 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hitoshi Nagatsuka: "Immunohistochemical study of Cytokeratin Patterns in Follicular and Plexiform Ameloblastoma" Journal of Hard Tissue Biology. 4. 50-56 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Kuboki: "Two Distinctive BMP-Carriers Induce Zonal Chondrogenesis and Membranous Ossification,Respectively ; Geometrical Factors of Matrices for Cell-Differentiation" Connective Tissue Research. 32. 219-226 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 今村高行: "骨誘導蛋白(rhBMP-2)による異所性骨形成と骨基質蛋白遺伝子の発現-In situハイブリダイゼーション法による検討-" 岡山歯学会誌. 14. 1-3 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hong-In Shin: "The Effect of Engymatic Treatment on Immunohistochemical Staining of Collagen in Mandibular Condyle of Rats" Journal of Hard Tissue Biology. 4. 8-14 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 永井教之: "アパタイト-骨結合界面の初期石灰化とBMPによる異所性骨誘導" Journal of Hard Tissue Biology. 4. 79-90 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masaru Murata: "Histopathological Studies of Heterotopic Bone Formations Induced by BMP Carrier Composites" Journal of Hard Tissue Biology. 3. 67-72 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toshiro Sakae: "Crystallographic properties and cell Afinity study of Synthetic α-Tricalcium phosphate ; Tricalcium Phosphate,Tetranalium" Journal of Hard Tissue Biology. 3. 73-78 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 長塚 仁: "BMP誘導異所性組織界面の骨芽細胞分化と骨基質蛋白遺伝子の発現-In Situ Hybridization法による検討-" J.Hard Tissue Biology. 3. 7-12 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 寒河江登志朗: "合成ハイドロキシアパタイトの結晶学的性質と細胞毒性試験" 日大口腔科学誌. 20. 40-48 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 永井教之: "Immnohistochemical Demonstration of Tenasin and fibronectin in Odontogenic Tumors and Human Fetal tooth Germs" J.Europian pathology(B)Oral oncology. 30. 191-195 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 井上 正久: "右口底部唾液腺腫瘍" 病院病理. 11. 126-126 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 日名 雅彦: "歯原性腫瘍のエナメル基質蛋白,IVコラーゲン,ファイブロネクチン局在の免疫組織化学的研究" 岡山歯学会雑誌. 13. 57-66 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 永井教之: "生体代替材料の組織界面の成立と異所性骨誘導蛋白BMPの登場" 東京都歯科医師会誌. 42. 11-21 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 長塚 仁: "Histopathological studies of Heterotopic Bone Formation Inducced by BMP-Collagen Bead carriers" 日本口腔インプラント学会誌. 7. 237-242 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 井上 正久: "Histopathologcal studies of heterotopic Bone Formation Inducced by BMP-membrane carrier composites" 日本口腔インプラント学会誌. 7. 243-248 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 林 勝彦: "エナメル上皮腫,悪性エナメル上皮腫の増殖細胞核抗原(PCNA)による免疫組織化学的検討" 日本口腔科学会雑誌. 44. 7-13 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 村田 勝: "Histopathological studies of Heterotopic Bone Fomations inducced by BMP-carriers compostites" J.Hard Tissue Biology. 3. 67-72 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 寒河江登志朗: "Crystallographic properties and cell Affinity Study of Synthetic α-tricalcium phosphate,β-tricallcium phosphate,tetracalcium phophate and fluorapatite" J.Hard Tissue Biology. 3. 73-78 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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