| Project/Area Number |
06454513
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
NAWA Tokio Iwate Med.Univ., Prof., 歯科学部, 教授 (50020748)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Hitoshi Iwate Med.Univ., Assist., 歯学部, 助手 (80265165)
FUJIWARA Naoki Iwate Med.Univ., Assist., 歯学部, 助手 (20190100)
SAKAKURA Yasunori Health Sci.Univ.Hokkaido, AP., 歯学部, 助教授 (60128915)
ISHIZEKI Kiyot Iwate Med.Univ., Lect., 歯学部, 講師 (50057775)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | Tissue culture / Tooth germ / Cartilage / Mechel's cartilage / Calcification / Meckel' s cartilage |
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| Research Abstract |
(1) On the enamel-free area : At the cusp tip of rodent molar, there is a region of dentin without an enamelcap which is colled enamel-free area (AFE). To clarify the cause of enamel-free area, we observed the ultrastructure of the basement membrane and the distal ends of the inner enamel epithelium in rats. On rats. On the dentin covered with enamel (DCE), collagen fibrils were arranged perpendicular to the basal lamina. On EFA,collagen fibrils ran parallel to the basal lamina. These finding suggested that the differences in the collagen arrangement between the EFA and DCE are associated with the developmental state of aperiodic microfibrils in the basal lamina. (2) On the osteogenic cell transformation of Meckel's cartilage chondrocytes : The Meckel's cartilage is a fetal organ with a resorptive ultimate fate soon after birth. However, we showed that when Meckel's cartilage was maintained in heterotopic site or cell culture, it expressed bone-type cellular element and matrix calcification. From these results in vivo and in vitro, it is clear that the cellular microenvironment such as extracellular matrix, cellular multiplication and blood promote the phenotypic conversion of Meckel's cartilage cells into osteogenic cells.
(3) On the development of the cellular cementum by the new organ culture method : We report a new organ culture method by which nearly normal development of root formation can be obtained. By this culture method the three-dimensional organogenesis and epithelial-mesenchymal interactions could be maintained.
In this study, we showed for the first time the formation of cellular cementum in a molar organ system in vitro.
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