| Project/Area Number |
06454515
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Matsumoto Dental College |
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Principal Investigator |
KAWAKAMI Toshiyuki Matumoto Dental College, Oral Pathology, Asociate Professor, 歯学部, 助教授 (80104892)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEI Noriyuki Matsumoto Dental College, Oral Pathology, Instructer, 歯学部, 助手 (60257508)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | BMP / evaluation / Image analysis / histological findings / squalane / osteoinduction / carrier / Mouse / マウス |
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| Research Abstract |
BMP is an important bioactive protein whose bioactivity is facilitated by the delivery system. In clinical application of endodontics, it is necessary to use fluid carrier for BMP.Squalane is easily emulsified and has the property of spreading well. Thus, squalane might be effective in a BMP delivery system. Squalane/BMP (bovine derived partially purified) composite were implanted under the perimuscular membrane of ddY mice. For control, BMP only were used. The implants were roentgenographically and histologically examined at 1 to 4 weeks. According to the roentgenographical analysis, squalane/BMP composite specimens showed widespread heterotopic bone formation, and the amount was about 40% greater than that in the controls by a statistically analysis. Histological examination revealed varying amounts of new bone formed by perichondral ossification by 2 weeks. Observation of the bone of 3- and 4-week specimens indicated that bone marrow had already formed. These data suggest that squalane is effective for the slow local release of BMP.
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