| Project/Area Number |
06454522
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
KATO Yukio Hiroshima Univ.School.Dent., Prof., 歯学部, 教授 (10112062)
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| Co-Investigator(Kenkyū-buntansha) |
NOSHIRO Mitsuhide Hiroshima Univ.School.Dent., Assoc.Prof., 歯学部, 助教授 (00144858)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | chondrocyte / PTH / collagen / adhesion / matrix metalloproteinase / PCR / cDNA / RGD配列 / TGF-B |
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| Research Abstract |
1. In the growth plate, chondrocytes ungergo a sequence of changes including proliferation, matrix synthesis, hypertrophy, and calcification. The calcified matrix is degraded, and replaced by bone, resulting in bone elongation. The rate of cartilage resorption is equal to the rate of growth, matrix formation and mineralization. However, factors regulating cartilage resorption have not been identified.
We found that PTH markedly stimulates MMP-2, MMP-3 and MMP-9 synthesis by hypertrophic chodrocytes, but not by articular chndorocytes.
These findings suggest that PTH/PTH-rp is crucial for cartilage resorption before ossification.
2. The primary structure of RGD-CAP cDNA was determined.
3. CMP was found to be a potent adhesion molecule for chondrocytes.
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