• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Difference in physiological roles of phosphorylated and non-phosphorylated rat fetuin.

Research Project

Project/Area Number 06454524
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionKagoshima University

Principal Investigator

DAIKUHARA Yasushi  Kagoshima University Dental School, Professor, 歯学部, 教授 (40028733)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Osamu  Kagoshima University Dental School, Research, Associate, 歯学部, 助手 (70128445)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsFetuin / Phosphorylated fetuin / Non-phosphorylated fetuin / alpha_2-HS glycoprotein / Hepatocyte growth factor (HGF) / Parathyroid hormone / Bone resorption / Chalone / α2-HS糖タンパク / リン酸化型ラットフェチュイン / 脱リン酸化型ラットフェチュイン / 組換え体発現 / 昆虫細胞
Research Abstract

In this project, we obtained following results.
1. Rat fetuin synthesized and secreted by hepatocytes was phosphorylated form and the rate of biosynthesis of this phosphorylated fetuin (phosphofetuin) was suppressed by inflammatory cytokines such as IL-1 (alpha and beta) and TNF-alpha, but it was stimulated by growth factors to hepatocytes such as hepatocyte growth factor (HGF), epidermal growth factor and insulin. This regulation of biosynthesis of phosphofetuin was confirmed both by the levels of protein and mRNA.
2. Phosphofetuin suppressed the biological activity of HGF which is known to be a potent mitogen to hepatocytes. However, non-phosphorylated fetuin did not show the activity to suppress the HGF activity. For the mechanism how phosphofetuin suppress the HGF activity, we found that phosphofetuin has affinity to bound HGF and thus phosphofetuin interfered with HGF binding to its specific receptor, c-Met. These observation suggest that phosphofetuin synthesized by hepatocytes may be a natural modulator of HGF as a 7chalone'.
3. Non-phosphorylated rat fetuin enhanced bone resorption induced by low concentrations (0.1-0.5nM) of parathyroid hormone (PTH), as measured by release of ^<45>Ca from ^<45>Ca-prelabeled rat radius, but it had no activity on bone resorption without PTH.We also found that rat non-phosphorylated fetuin enhanced the digestion of type I collagen by cathepsin L.
4. Although bovine fetuin and its human counterpart of alpha_2-HS glycoprotein is known to be a plasma sialoprotein for long time, its physiological role is not yet clear. From above observations using rat fetuin, we found one of physiological roles of phosphorylated and non-phosphorylated fetuin/alpha_2-HS glycoprotein.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] T.Ohnishi: "Effects of cytokines and grawth factors on phosphorylated fetuin biosynmthesis by adult rat hepatocytes in primary----." Biochem. Biophys. Res. Commun.200. 598-605 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大工原恭(分担): "肝疾患と肝細胞増殖因子(肝疾患と細胞増殖因子、黒木登志夫編)" メジカルレビュー社, 136-150 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Ohnishi, et al.: "Effects of cytokines and growth factors on phosphorylated fetuin biosynthesis by adult rat hepatocytes in primary culture." Biochem. Biophys. Res. Commun.200. 598-605 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Daikuhara: Liver diseases and hepatocyte growth factor. Liver Diseases and Growth Factor (T.Kuroki, ed.). Medical View, Tokyo, 136-150 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Ohnishi: "Effects of cytokines and growth factors on phosphorylated fetuin biosynthesis by adult rat hepatocytes in primary…." Biochem.Biophys.Res.Commun.200. 598-605 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大工原恭(分担): "「肝疾患と肝細胞増殖因子」(肝疾患と細胞増殖因子,黒木登志夫編)" メジカルレビュー社, 136-150 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ohnishi,T.: "Effects of cytokines and growth factors on phosphorylated fetuin biosynthesis by adult rat hepatocytes in primary culture." Biochem.Biophys.Res.Commun.200. 598-605 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sakaguchi,H.: "Ultrastructural location of human hepatocyte growth factor in human liver" Hepatology. 19. 1157-1163 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawakami,S.: "Expression of hepatocyte growth factor in normal and carbon tetrachloride-treated monkeys." Hepatology. 20. 1225-1260 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamada,T.: "Immunohistochemistry with antibodies to hepatocyte growth factor and its receptor (c-Met) in human brain tissues." Brain Res.637. 308-312 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大工原 恭: "ヒト肝細胞増殖因子の発現と臨床的意義" 日本臨床化学会雑誌. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大工原 恭: "細胞増殖因子と疾患(黒木登志夫編),分担" メジカルレビュー社(印刷中), (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 中村 修: "タンパク質化学 第5巻,血漿タンパク質(長沢滋治編),分担" 広川書店(印刷中), (1995)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi