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Apoptosis of macrophages and T cells in periodontal tissues : Its molecular mechanisms and biological roles

Research Project

Project/Area Number 06454528
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

KIZAKI Harutoshi  Tokyo Dental College, Professor, 歯学部, 教授 (60051653)

Co-Investigator(Kenkyū-buntansha) TANIMOTO Yutaka  Tokyo Dental College, Instructor, 歯学部, 助手 (10276975)
ONISHI Yoshiaki  Tokyo Dental College, Instructor, 歯学部, 助手 (60233219)
東 祐太郎  東京歯科大学, 生化学講座, 助手 (80231918)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥6,100,000 (Direct Cost: ¥6,100,000)
KeywordsPeriodontitis / Apoptosis / Monocytes / Macrophages / T cells / Thymocytes / Tyrosine phosphorylation / LPS / 歯周組織 / 分子機構
Research Abstract

Apoptosis is a physiological cell death process of eliminating unwanted cells from living organisms during embryogenic and adult development. It also function in regulation of survival of inflammatory cells in chronic inflammatory lesions. The aims of the present study are to elucidate the molecular mechanisms and roles of apoptosis of monocytes, macrophages and T cells which play important roles in periodontitis. Thymocyte apoptosis is found to be regulated by at least two steps of protein phosphorylation ; one is triggering apoptosis, another is involved in progressing apoptosis. The latter protein phosphorylation occurs at a later stage of the process and seems to be common to the apoptosis induced by various stimuli. Thymocyte apoptosis induced by various stimuli was inhibited by inhibitors of proteasome, suggesting an important role of proteases in apoptosis. Apoptosis induced by dexamethasone in spleen T cells was paritially inhibited by cycloheximide, though cycloheximide itself … More induced apoptosis. Thus, there exist two mechanisms of apoptosis in mature T cells, protein synthesis-dependent and-independent. Spleen T cells were resistant to TPA or A23187 which was able to induce apoptosis in thymocytes. However, T cells bacame relatively sensitive to A23187 after cultivation for 3 days in the presence of IL-2, suggesting that activation-induced cell death is induced in part by calcium-mediated signaling. IL-2-dependent CTLL-2 cells underwent apoptosis when IL-2 was withdrawn accompanying by decreased in protein tyrosine phosphorylation in nuclear protein. Depletion of IL-2 is a cause of activation-induced cell death which participates in resolution of inflammation. Further studies on the molecular mechanisms of apoptosis in thymocytes and T cells are required. Monocyte and macroophage cell lines, U937 and P388D1 cells underwent apoptosis when serum was depleted. The apoptosis was inhibited by TNF-alpha or substance P accompanying by increased protein tyrosine phosphorylation. The results suggest cytokines or neuropeptides modualate inflammatory responses through regulating survival of inflammatory cells. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] 木崎治俊: "歯科疾患とプログラム細胞死"アポトーシス"" ザ・クインテッセンス. 14. 48-50 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 木崎治俊: "アポトーシスと病気" 組織培養. 21. 90-93 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大西芳秋: "アポトーシスの多様性とその制御機構" 臨床免疫. 27. 308-316 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 東祐太郎: "免疫系アポトーシスの分子機構" 日本臨床. 53. 753-760 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 木崎治俊: "アポトーシスと細胞診" 日本臨床細胞学会東京都支部会報. 13. 17-20 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 東祐太郎: "T細胞" 炎症と免疫. 2. 295-300 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki Kazuko: "Biphasic effect of staurosporine on thymocyte apoptosis" Biochem. Mol. Biol. Int.35. 1085-1092 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Azuma Yutaro: "Staurosporine-induced thymocyte apoptosis is inhibited by herbimycin A,s specific inhibitor of protein tyrosine kinase." Biochem. Mol. Biol. Int.37. 635-643 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Azuma Yutaro: "Effect of protein tyrosine kinase inhibitors with diferent modes of action on topoisomerase acitvity and death of IL-2 dependent CTLL-2 cells." J. Biochem. 118. 312-318 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 木崎治俊: "アポトーシスと医学" 実験医学. 13. 1972-1976 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大西芳秋: "アポトーシスとは" 臨床医. 21. 2076-2079 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 木崎治俊: "アポトーシスの概念" プリン・ピリミジン代謝. 19. 163-168 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 木崎治俊: "アポトーシス-細胞機能と分子機構-" 中外医学社, 265 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 橋本嘉幸: "アポトーシスの分子医学" 羊土社, 165 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 山田武: "アポトーシス" 日経サイエンス, 213 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 柏崎禎夫: "リウマチ'95" メディカルレビュー社, 174 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yutaro Azuma: "T cells (in Japanese)" Inflammation & Immunology. 2. 295-300 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Harutoshi Kizaki: "Oral diseases and programd cell death "apoptosis" (in Japanese)" Quintessence. 14. 48-50 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Harutoshi Kizaki: "Apoptosis and disease (in Japanese)" Tissue Culture. 21. 90-93 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshiaki Onishi: "Multiple mechanisms of apoptosis (in Japanese)" Clinical Immunology. 27. 308-316 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yutaro Azuma: "Molecular mechanisms of apoptosis in the immune systems" Nippon Rinsho. 53. 753-760 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Harutoshi Kizaki: "Apoptosis and medicine (in Japanese)" Experimental Medicine. 13. 1972-1976 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshiaki Onishi: "Apoptosis (in Japanese)" Medical Clinics of Japan. 21. 2076-2079 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Harutoshi Kizaki: "Concept of Apoptosis (in Japanese)" Purine and Pyrimidine Metabolism. 19. 163-168 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuko Suzuki: "Biphasic effect of staurosporine on thymocytes apoptosis" Biochem Mol Biol Int. 35. 1085-1092 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yutaro Azuma: "Staurosporine-induced thymocyte apoptosis is inhibited by herbimycin A,a specific inhibitor of protein tyrosine kinase." Biochem Mol Biol Int. 37. 635-643 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yutaro Azuma: "Effect of protein tyrosine kinase injhibitors with different modes of action on topoisomerase activity and death of IL-2 dependent CTLL-2 cells." J Biochem. 118. 312-318 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Harutoshi Kizaki: Apoptosis-cellular function and molecular mechanisms. Chugai Igaku-sha, 265 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshiyuki Hashimoto: Molecular Medicine of Apoptosis. Yoodo-sha, 165 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tekashi Yamada: Apoptosis. Nikkei Science, 213 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki Kazuko: "Biphasic effect of staurosporine on thymocyte apoptosis" Biochem. Mol. Biol. Int.35. 1085-1092 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Azuma Yutaro: "Staurosporine-induced thymocyte apoptosis is inhibited by herbimycin A, s specific inhibitor of protein tyrosine kinase." Biochem. Mol. Biol. Int.37. 635-643 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Azuma Yutaro: "Effect of protein tyrosine kinase inhibitors with diferent modes of action on topoisomerase acitvity and death of IL-2 dependent CTLL-2 cells." J. Biochem. 118. 312-318 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 木崎 治俊: "アポトーシスと医学" 実験医学. 13. 1972-1976 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大西 芳秋: "アポトーシスとは" 臨床医. 21. 2076-2079 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 木崎 治俊: "アポトーシスの概念" プリン・ピリミジン代謝. 19. 163-168 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 山田 武: "アポトーシス" 日経サイエンス, 213 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 柏崎 禎夫: "リウマチ '95" メディカルレビュー社, 174 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 東祐太郎: "アポトーシス-実体とその意義-T細胞" 炎症と免疫. 2. 295-300 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 木崎治俊: "歯科疾患とプログラム細胞死"アポトーシス"" ザ・クインテッセンス. 14. 48-50 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大西芳秋: "アポトーシスの多様性とその制御機構" 臨床免疫. 27. 308-316 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 木崎治俊: "アポトーシスと疾患" 組織培養. 21. 90-93 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suzuki,K.: "Biphasic effect of staurosporine on thymocyte apoptosis" Biochem.Mol.Biol.Internatl.in press. (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 東祐太郎: "免疫系のアポトーシスの分子機構" 日本臨床. 53. 753-760 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 木崎治俊: "アポトーシス-細胞機能と分子機構" 中外医学社, 265 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 橋本嘉幸: "アポトーシスと医学" 羊土社, 165 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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