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An Experimental Study of the Periapical Healing after Apexification on the Immature Pulpless Teeth

Research Project

Project/Area Number 06454548
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionTHE NIPPON DENTAL UNIVERSITY

Principal Investigator

IGARASHI Masaru  THE NIPPON DENTAL UNIVERSITY,SCHOOL OF DENTISTRY AT NIIGATA ASSOCIATE PROFESSOR, 新潟歯学部, 助教授 (90168104)

Co-Investigator(Kenkyū-buntansha) MIYASATO Tsuyoshi  THE NIPPON DENTAL UNIVERSITY,SCHOOL OF DENTISTRY AT NIIGATA ASSISTANT, 新潟歯学部, 助手 (00239378)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥4,700,000 (Direct Cost: ¥4,700,000)
KeywordsImmature tooth / Pulpless tooth / Apexification / Periapical healing / Calcium hydroxide / Macaca irus / Animal experiment / Apical closure / 根尖創傷治療 / 創傷治癒 / 動物実験
Research Abstract

Periapical wound healing after apexification on immatured non-vital permanent teeth with bulunderbass canals were evaluated histologically. This investigation was undertaken on sound permanent teeth in three crab-eating young mondeys aged four approximately.
Under general and local anesthesia, access openings of the teeth were performed with rubber-dam isolation, then the coronal pulps were extirpated. Remnant root pulps were cauterized and coagulated with high frequency current, and then divided to two groups, one was infected group, and other was non-infected groop.
One week later, infected root canal treatment was started, and calcium hydroxide or FR-Ca paste was applicated to the canal after root canal disinfection. At the conclusion of the experimental period, 1,3,5 months, the animals were sacrificed by perfusion with neutral formalin. Decalcified serial paraffin sections were prepared and stained with hematoxylin-eosin, and observed by light microscopy.
The results obtained were as follows :
1. The pulp tissue which contacted with eletrode was coagulated, but at some distance from it vital pulptissues were still existent in alive.
2. The burn caused by a rise in temperature of the root conal was observed in the periodontal ligaments and alveolar bone on the immature tooth with thiner root canal wall, and external root resorption and/or ankylosis with the bone were observed.
3. After the apexification with calcium hydroxide on non-infected group, addition of severe osteo-dentin which filled up the canal space was observed at apex area.
4. Continual development of the root by osteo-dentin was observed, but abnormal formation of root apex and severe calcified of root canals were appeared.
In future, these results will be compared with infected groop, and details will be investigated further.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 五十嵐 勝: "歯根未完成無髄歯のApexification後における創傷治癒に関する実験的研究" 日本歯科保存学雑誌. 39(予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaru IGARASHI,Tsuyoshi MIYASATO & Kohichi KAWASAKI: "An Experimental Study of the Periapical Healing after Apexificati on on the Immature Pulpless Teeth" J of Japan.conservative dentistry. 39. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 五十嵐 勝: "歯根未完成無髄歯のApexification後における創傷治癒に関する実験的研究" 日本歯科保存学雑誌. 39(予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 五十嵐 勝: "歯根未完成無髄歯のApexification後における創傷治癒に関する実験的研究" 日本歯科保存学雑誌. 39(予定). (1996)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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