| Project/Area Number |
06454578
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Kurume University |
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Principal Investigator |
KAMEYAMA Tadamistu Kurume University Medical department, Professor, 医学部, 教授 (00080937)
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| Co-Investigator(Kenkyū-buntansha) |
HUJITA Hiroshi Kurume University Medical department, Assistant, 医学部, 助手 (10248453)
TERASAKI Sinitirou Kurume University Medical department, Assistant, 医学部, 助手 (70227510)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1994: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | herpes simplex virus / type 1 / superinfection / latent DNA / restiction enzyme / DNA cleavage pattern / viral clone |
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| Research Abstract |
In order to clarified a latent superinfection of herpes simplex virus type 1 (HSV-1), we carried out restriction endonuclease analysis of DNA for stock viral strains of HSV-1 which were isolated on different occations from the erosion or vesicle of herpetic infection and the saliva of oral cancer patients without herpetic lesions in each individuals.
Also, we compared DNA cleveage patterns of four and five viral clones which were extracted from the isolated viruses of two groups above-mentioned.
The results obtained as follows.
1) All viral strains in each individuals of HSV-1 infections group showed identical DNA cleveage patterns.
2) On the other hand, 9 of 13 subjects in the asymptomatic group shed at more three weeks interval showed different DNA cleavage patterns.
We thought that this had clearly demonstrated the presence of a latent superinfection for HSV-1.
2) 2 of 10subjects in oral cancer patients without herpetic lesion sheded into saliva everyday showed different DNA cleavage patterns in viral strain shed into saliva at last day.
Also, in 1 of 2 subjects who were able to extracted viral clone from saliva asymptomatically, two viral strains showed different DNA cleavage patterns were recognized.
These results suggested the existence of a latent superinfection of HSV-1 and the fact that plural strains of HSV-1 shed into saliva simultaneously.
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